Effect of abiotic elicitors on Carvone metabolite production in cell suspension culture of Black Cumin (Bunium persicum Boiss)
Subject Areas : Biotechnology
Zahra aghamiri
1
,
Parisa Abdollahi
2
,
Mahmood Khosrowchahli
3
1 - Department of plant breeding and Biotechnology, Science and Research Branch, Islamic Azad University, Tehran, Iran
2 - Department of plant breeding and Biotechnology, Science and Research Branch, Islamic Azad University, Tehran, Iran
3 - Professor of Genetics Department of plant Breeding, Faculty of Agriculture and Natural Resources, Tehran Azad University, Science and Research Branch, Tehran IRAN. Phone (home): 00982613405366, Fax: 00982613410416, Mobile phone:00989123611248
Keywords: Methyl jasmonate, Salicylic acid, Tio2 nano particle, Secondary metabolite, In vitro culture ,
Abstract :
were germinated on agar medium containing BAP at a concentration of 0.25 mg/L after 20 days. Explants of cotyledons, hypocotyls and roots of black cumin were placed on MS medium containing 2,4-D growth regulator at three concentrations of 1,2 and 4 mg/L for callus induction. The results showed that 1 mg/L of 2,4-D concentration had the best effect on dry weight, fresh weight and callus size. For carvone metabolite production, black cumin cell suspension culture was prepared using the obtained callus. The elicitors methyl jasmonate, nano titanium oxide and salicylic acid were used in two concentrations of 100 and 150 mg/L and were added to the suspension after 14 days and the cells were harvested 24 hours after the elicitor application. This experiment was conducted in a completely randomized design with three replications. Methyl jasmonate increased the amount of carvone to 2399.16 μg/mg of dry mass, which was significantly different from the control sample. Although the amount of carvone production in the treatment with salicylic acid and titanium oxide nanoparticle elicitors was less than its production by methyl jasmonate still was higher than the control and showed a significant difference. In general, the results indicated a positive effect of elicitors on the in vitro production of cumin plant.
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