The effect of interval training on the expression of mitochondrial transcription factor (TFAM) in the gastrocnemius muscle tissue of male Wistar rats exposed to cigarette smoke extract
Subject Areas : Plasma biomarkers
Mehdi Bakhshi
1
,
عبدالعلی بنائی فر
2
,
Sajad Arshadi
3
,
Behzad Bazgir
4
1 - PhD student in Exercise Physiology, Faculty of Physical Education, Islamic Azad University, South Tehran Branch, Tehran, Iran.
2 -
3 - Assistant Professor of Exercise Physiology , South Tehran Branch , Islamic Azad university, Tehran, Iran.
4 - baqiyatallah university of medical sciences
Keywords: Interval training, gastrocnemius muscle tissue, cigarette smoke extract, gene expression, TFAM,
Abstract :
Background and aim: Studies show that exposure to cigarette smoke can inhibit protein synthesis by increasing proteolysis of muscle tissue and disrupt factors affecting mitochondrial function. The aim of the present study was to study the effect of interval training on TFAM expression in male Wistar rats exposed to cigarette smoke extract.
Materials and Methods:
In this study, 28 male Wistar rats (8 weeks old) with an average weight of 220.12 ± 10.80 g were selected and divided into four groups: control, cigarette smoke extract, exercise, and exercise + cigarette smoke extract. Cigarette smoke extract (CSE) contained 12 mg of tar and 0.9 mg of nicotine. The saline control group and the CSE group were injected with cigarette smoke extract one day a week for 6 weeks. The interval training protocol was performed for 49 minutes per session, 5 times a week for 6 weeks. After the last training session, the rats were anesthetized and their muscle tissue was isolated and transported to the laboratory. Then, the expression of the target gene was measured. The findings were analyzed with ANOVA and the LSD post hoc test was used (p≥0.05).
Results:
The results showed that TFAM expression in the cigarette smoke extract group showed a significant decrease compared to the healthy control group (P=0.046). However, there was no significant difference in the exercise group and the exercise + cigarette smoke extract group compared to the other groups (P≥0.05).
Conclusion: The results showed that cigarette smoke extract caused a significant decrease in TFAM expression in the gastrocnemius muscle. It also seems that intermittent training with cigarette smoke, by increasing TFAM function, can reduce the effects of oxidative stress caused by cigarette smoke extract and protect mtDNA against ROS damage and help improve mitochondrial biogenesis.
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