Enhancing Callus Growth in Calla Lily (Zantedeschia ‘Sun Club’) through Fipexide, Activated Charcoal, and Ascorbic Acid in In Vitro Cultivation
Subject Areas : Journal of Ornamental PlantsHassan Abedini 1 , Pejman Azadi 2 , Mohammad Hosein Azimi 3 , Sepida Kalate Jari 4 , Azam Borzouei 5
1 - Department of Horticultural Science and Agronomy, Science and Research Branch, Islamic Azad University, Tehran, Iran
2 - Department of Genetic Engineering and Biosafety, Agricultural Biotechnology Research Institute of Iran (ABRII), Agricultural Research, Education and Extension Organization (AREEO). Karaj, Iran
3 - Ornamental Plants Research Center (OPRC), Horticultural Sciences Research Institute (HSRI), Agricultural Research, Education and Extension Organization (AREEO), Mahallat, Iran
4 - Department of Horticultural Science and Agronomy, Science and Research Branch, Islamic Azad University, Tehran, Iran
5 - Agriculture Research School, Nuclear Science and Technology Research Institute, PO Box 31485-498, Karaj,Iran
Keywords: Crown calli, Geophyte, Ornamental plant, Pot plant, Plant tissue culture,
Abstract :
The potted calla lily (Zantedeschia spp.) is a popular ornamental plant in the global flower market. The most important problem of calla lily in indirect organogenesis is callus production, low durability and growth of callus due to the phenolic and alkaloidal compounds present. For this purpose, an experiment was conducted to investigate the effect of fipexide (FPX) compared with activated charcoal and ascorbic acid in the culture medium to improve the quality of the callus of the potted calla lily (Zantedeschia ‘Sun Club’). FPX is one of the chemical compounds used in the pharmaceutical industry. Recently, for in vitro conditions FPX was used in the culture media to improve the quality of different stages of plant growth. A factorial experiment in the form of a completely randomized design including FPX at concentrations of 0, 15 and 30 μmol L-1, activated charcoal (0 and 1 g L-1) and ascorbic acid at concentrations of 0 and 2 g L-1, including 12 treatments, 3 replications, 12 samples for each treatment and in the collection with 192 callus samples were implemented. In this research, callus diameter, callus fresh weight, callus growth index and healthy callus percentage were evaluated. The results showed that, FPX treatment, especially at a concentration of 15 μmol L-1 with activated charcoal, had the greatest effect on the examined traits such as callus diameter (15.98 mm), healthy callus percentage (90%) compared to the control samples (with an average of 7.36 mm and 54 % respectively). Totally, FPX can be used as an effective and alternative compound in the callus growth medium to increase quality and performance.
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