Studing the effect of ascorbic acid on the physiological and biochemical characteristics of chamomile ( Matricaria chamomilla L.) under salt stress
Subject Areas : Tension
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Keywords: Chamomile, Chlorophyll, Mineral salts, Osmotic regulators, Salt stress.,
Abstract :
Salinity stress is one of the important factors of reducing yield and production in agricultural horticultural and medicinal plants in the word. Ascorbic acid , as a strong antioxidant , can be effective in improving the performance of plants under stress conditions. Its application under salinity stress conditions decreases the effects of salinity toxicity and makes resistance in the plants. To investigate the chamomile (Matricaria chamomilla) physiological and biochemical responses to salinity stress and ascorbic acid , a factorial pot experiment was conducted based on a completely randomized design with three replications in the research greenhouse of Shahrood University Of Technology. Experimental treatments included salinity at four levels (0, 30 ,60, and 90 mM) and ascorbic acid at five levels (0, 0.5, 1, 1.5, and 2 mM). The results showed that salinity stress reduced the amount of chlorophyll florescence, chlorophylls a and b, total chlorophyll, relative water content, and essential oil percentage. The ascorbic acid treatment decreased the negative effects of salinity stress on the plant and improved essential oil percentage. Also, salinity stress increased the amount of proline, soluble carbohydrate, electrolyte leakage, leaf and root sodium, and chlorine, but the ascorbic acid treatment at some salinity stress levels caused to decrease and/or increase these traits. Overall, ascorbic acid improved the physiological and biochemical characteristics of basil under salinity stress through increasing osmolytes production and resulted in increasing nutrient uptake and essential oil percentage of the plant.
Abedi, T. and Pakniyat, H. (2010). Antioxidant enzyme changes in response to drought stress in ten cultivar of oilseed rap (Brassica napus L.). Czech Journal of Genetics and Plant Breeding, 46(4): 27-34.
-Agami, R.A. 2014. Applications of ascorbic acid or proline increase resistance to salt stress in barley seedlings. Biologia Plantarum.58,341-347
- Ahmadi, A. and Siose Marde, A. (2004). Effect of drought stress on soluble carbohydrates, chlorophyll and proline in four wheat cultivars adapted to different climatic conditions of Iran. Iranian Journal of Agriculture Sciences. 35(3): 753-763.
- Amiri, H. and Moazeni, L.( 2016). Interaction of salinity and ascorbic acid with some biochemical features in Satureja khuzistanica. Nova Biologica Reperta. 3(1): 69-79.
- Arnon, D.I.( 1949). Copper enzymes in isolated chloroplasts. Polyphenol oxidase in Beta vulgaris. Plant Physiology. 24: 1-15.
- Barrert-Lennard, E.G. (2003). The interaction between waterlogging and salinity in higher plants: causes, consequences and implications. Plant and Soil. 253(1): 35-54.
- Bastam, N., Baninasab, B. and Gobadi, C. (2012). Improving salt tolerance by exogenous application of salicylic acid in seedlings of pistachio. Plant Growth Regulation. 18: 206-211.
-Beltagi, M.S.( 2008). Exogenous ascorbic acid (vitamin C) induced anabolic changes for salt tolerance in chick pea ( Cicer arietium L.) plants. African Journal of Plant Science. 2(10):118-123.
-Chaparzadeh, N., Khavari-Nejad, R. A., Navari-Izzo, F. and Izzo, R. (2003). water relatios and ionic balance in calendula officinalis L. under saliniyy conditions. Agrochimica. 47(1/2): 69-79.
- Chapman, H.D. and Pratt, P.F.( 1961). Methods of Analysis for Soils, Plants and Waters. University of California, Los Angeles, 309p.
- Chawla, S., Jain, S. and Jain, V. (2013). Salinity induced oxidative stress and antioxidant system in salt-tolerant and salt-sensitive cultivars of rice (Oryza sativa L.). Journal of Plant Biochemistry and Biotechnology,
-Conkin, P.L. ( 2001) . Recent advances in therole and biosynthesis of ascorbic acid in plants. Plant, cell and Environment,24(4),383-349. https”// dio.org/10.1046/j.1365-3040.2001.00686.x, 22(1): 27-34.
-Daneshmand, F.( 2013). The effect of ascorbate pre-treatment on tomato plant under drought stress: oxidative stress, osmolytes, Phenolics and protein. Iranian Journal of Plant Biology. 5(18): 53-66 (in Persian).
- Deef, H.E. 2007. Influence of ascorbic acid on stress tolerance during seed germination of Triticum aestivum and Hordeum vulgare. Advances in Biological Research. 1(12): 40-48.
- Demiral, M.A., Aydin, M. and Yorulmaz, A. (2005). Effect of salinity on growth chemical composition and antioxidative enzyme activity of two malting barley (Hordeum vulgare L.) cultivars. Turkish Journal of Biology. 29(2): 117-123.
El-Hariri, D. M., Sadak, M.S. and El- Bassiouny, H.M.S.( 2010). Responses of flux cultivars to ascorbic acid under salinity stress condition. International Journal of Academic Research. 2(6):101-109.
- Emarat Pardaz, J., Hami, A. and Gohari, Gh. (2014). Evaluation of growth characteristics and yield of savory essential oil (Satureja hortensis L.) under salinity and zinc foliar treatment. Agricultural science and sustainable production. 26(3): 131-141.
- Eraslan, F., Inal, A., Gunes, A. and Alpaslan, M. (20070. Impact of exogenous ascorbic acid on the growth, antioxidant activity and physiology of carrot plants subjected to combined salinity and boron toxicity. Scientia Horticalturae. 113(2): 120-128.
- Gharib, F.A. (2007.) Effect of ascorbic acid on the growth, metabolic activities and oil content of basil and marjoram. International Journal of Agriculture and Biological. 2: 294-301.
- Haghnia, Gh.H., 1993. Guide to Plant Tolerance to Salinity. Jihad Daneshgahi (Ferdowsi University of Mashhad), 630p.
- Harati, E., Kashefi, B. and Matinizadeh. M.( 2015). Investigation of reducing detrimental effects of salt stress on morphological and physiological traits of (Thymus daenensis Celak.) through salicylic acid application. Journal of Plant Production Technology. 16(2): 111-125.
- Hasani, A. and Omidbeigi, R. (2003). Effects of water stress on some morphological, physiological, and metabolical characteristics in basil (Ocimum basilicum). Journal of Agricultural Science. 12(3):47-59.
- Hosseini, H., Mousavi Fard, S., Fatehi, F. and Qaderi, A.( 2016). Phytochemical changes and morpho-physiological traits of Thymus vulgaris L. cv. Varico 3 under salinity stress. Medicinal Plants, 16(10):22-33.
- Hussein, M.M., Balbaa, L.K. and Gaballah, M.S.( 2007). ascorbic acid and salinity effects on growth of maize plant. Journal of Agriculture and Biological Science. 34: 321-328.
- Irigoyen, J.J., Emerich, D.W. and Sanches-Disz, M. (1992). Alfalfa leaf senescence induced by drought stress, photosynthesis, hydrogen peroxide metabolism, lipid peroxidation and evolution, Physiologia Plantarum. 84(1): 67-72.
- Jahan Tigh, A., Najafi, F., Naqdi Badi, H.A., Khavarinejad, R.A. and Sanjarian, F. (2016). Study of some physiological indicators of Hyssopus officinalis in the vegetative stage under the influence of salinity stress. Plant Biology. 8(27): 81-94.
- Karimi, G., Ghorbanli, M., Heidari, H., Khavarinejad, R.A. and Assareh, M.H.( 2005). The effects of NaCl on growth, water relations, osmolytes and ion content in Kochia prostrata. Biologia Plantarum. 49(2): 301-304.
Khan, H.A., Pervez, M.A., Avub, C.M., ziaf, K., Balal, R.M., Shahid, M.A., and Akhtar,N. (2009). Hormonal priming alleviates salt stress in hot pepper (Capsicum annum L.). Soil and environment,28(2),130-135.
- Korkmaz, A., Uzunlu, M. and Demirkiran, A.R. (2007). Treatment with acetyl ascorbic acid protects muskmelon seedlings against drought stress. Acta Physiologiae Plantarum. 29(6): 503-508.
- Manchanda, G. and Garg, N. (2008). Salinity and its effects on the functional biology of legumes. Acta Physiologiae Plantarum. 30(5): 595-618.
- Moghaddam, M. and Talebi, M. (2016). The effects of salinity and methyl jasmonate on morphological and biochemical characteristics and photosynthetic pigments content in two basil cultivars. Seed and Plant Production Journal. 32(1): 81-98.
Munns, R., James, R.A. and Lauchli. A.( 2006). Approaches to increasing the salt tolerance of wheat and other cereals. Journal of Experimental Botany. 57(5): 1025-1043.
- Naghavi, M.R., Maroof, Kh. and Aboughadereh, A.R. (2015). Effect of water deficit stress on yield and yield components of canola (Brassica napus L.) cultivars. International Journal of Agronomy and Agricultural Research (IJAAR), 7(2): 8-13.
-Narimai, R., moghadam, M. and Nematy, H.( 2017). Evaluation of adjusted by using humic acid and ascorbic acid in medicinal plant of moldavian balm (Dracocephalum moildavica L.). Journal Plant Reasearch. 31(40):955-971.
- Narimani, R., Moghaddam, M. and Ghasemi Pirbalouti, P.( 2020). Phytochemical changes of Dracocephalum moldavica L. essential oil under different salinity stresses and application of humic and ascorbic acid. Eco-phytochemical Journal of Medicinal Plants. 7(4): 34-48.
- Noorani Azad, H. and Hajibagheri, M.R. (2008). Effect of salinity stress on some physiologic characteristics of dill (Anethum graveolens L.). Agroecology Journal. 4(12): 93-100.
-Omidbeigy, R. (2009). Payyin attention to medicinal plants in low- use stangic land for sustainable employment generation.The first national conference on management and development of sustainable agriculture in Ahvaz , Iran.407-410.
- Paquin, R. and Lechasseur, P. (1979). Observations on a method for determining free proline in plant extracts. Canadian Journal of Botany. 57(18): 1851-1854.
Saed-Moucheshi, A., Shekoofa, A. and Pessarakli, M.( 20140. Reactive oxygen species (ROS) generation and detoxifying in plants. Journal of Plants Nutrition. 37: 1573-1585.
- Scagel, C.F., Lee, J. and Mitchella, J.N. (2019). Salinity from NaCl changes the nutrient and polyphenolic composition of basil leaves. Industrial Crops and Products. 127: 119-128.
Sheteawi, S. (2007). Improving growth and yield of salt- stressed soybean by exogenous application of jasmonic acids and ascorbin. International Journal of Agriculture and Biology. 9:473-478.
- Tabatabaie, J. and Nazari, J. (2007). Influence of nutrient concentrations and NaCl salinity on the growth, photosynthesis and essential oil content of peppermint and lemon verbena. Turkish Journal of Agriculture and Forestry. 31(4): 245-253.
- Tavallaie, V., Rahemi, M., Eshghi, S., Kholdbarin, B. and Ramezanian, A. (2010). Zinc alleviates salt stress and increases antioxidant enzyme activity in the leaves of pistachio (Pistacia vera L. Badami) seedlings. Turkish Journal of Agriculture and Forestry. 34(4): 349-359.
- Vatankhah, E., Kalantari, B. and Andalibi, B.( 2017). Effects of methyl jasmonate and salt stress on physiological and phytochemical characteristics of peppermint (Mentha Piperita L.). Iranian Journal of Medicinal and Aromatic Plants Research. 33(3): 449-465.
- Weatherley, P.E. (1950). Studies in water relation of cotton plants, the field measurement of water deficit in leaves. New Phytologist. 49: 81-87.
