Unveiling Key Factors in Walnut Grafting Success: The Impact of Grafter Skill and Budding Method
Rasoul Sadeghi-Majd
1
(
)
Mahmoud Roozban
2
(
)
Saadat Sarikhani
3
(
)
Maryam Nowrouzi
4
(
)
Kourosh Vahdati
5
(
)
Keywords: Flute budding, Grafter expertise, Grafting success, Persian walnut, Vegetative propagation,
Abstract :
Achieving reliable grafting success in Persian walnut (Juglans regia L.) remains challenging due to complex environmental and technical factors. This study examines how the skill of the grafter and the budding technique affect grafting results, offering valuable insights for enhancing walnut propagation practices. We compared two budding methods—patch and flute—applied by grafters with varied experience levels. Results reveal that flute budding, performed by experienced grafters, significantly enhanced callus formation quality, bud take, and overall graft survival rates. The flute method yielded a 94.4% bud-take rate and a 90% graft survival, outperforming patch budding, which achieved 90% and 81.1%, respectively. Notably, the combination of flute budding and the most skilled grafter reached a 100% survival rate, underscoring the impact of technique and expertise on propagation success. These findings highlight grafter proficiency as a key factor in walnut grafting, suggesting that targeted training and the adoption of flute budding could improve propagation efficiency. This study contributes valuable knowledge to walnut propagation, proposing straightforward protocols to enhance the success of vegetative propagation in commercial and research settings.
Atefi J (1997) Comparison of hypocotyle and hot callus cable graft with traditional grafting method. Acta Horticiculyure. 442,309–313. https://doi.org/10.17660/ActaHortic.1997.442.48
Dehghan B, Vahdati K, Hassani D, Rezaee R (2010) Bench-grafting of Persian walnut as affected by pre-and postgrafting heating and chilling treatments. The Journal of Horticultural Science and Biotechnology. 85(1), 48-52. https://doi.org/10.1080/14620316.2010.11512629
Ebrahimi A, Vahdati K, Fallahi E (2007) Improved success of Persian walnut grafting under environmentally controlled conditions. International Journal of Fruit Science. 6(4), 3-12. https://doi.org/10.1300/J492v06n04_02
Ertürk U (2013) Effect of cultivar on successful grafting and development of nursery trees of potted walnut. Acta Horticulture. 981, 443–447. https://doi.org/10.17660/ActaHortic.2013.981.70
Farsi M, Fatahi Moghadam, MR, Zamani Z, Hassani D (2018) Effects of scion cultivar, rootstock age and hormonal treatment on minigrafting of Persian walnut. International Journal of Horticultural Science and Technology. 5(2), 185-197. https://doi.org/10.22059/ijhst.2018.255460.233
Gandev S, Arnaudov V (2011) Propagation method of epicotyl grafting in walnut (Juglans regia L.) under production condition. Bulgarian Journal of Agricultural Science. 17(2), 173-176.
Ghamari HF, Sharafi Y, Tabatabaei SJ, Grigurian V (2016) Effect of budding method, rootstock age and cut below budding union on budding success in Persian walnut. Journal of Nuts. 7(2), 119-124.
Habibi A, Sarikhani S, Arab MM, Soltani M, Aliniaifard S, Roozban MR, Vahdati K (2024) Drought and Heat Stress Interactions: Unveiling the Molecular and Physiological Responses of Persian Walnut. Plant Physiology and Biochemistry. 109237. https://doi.org/10.1016/j.plaphy.2024.109237
Habibie A, Yazdani N, Saba MK, Vahdati K (2021) Limitation of access to oxygen for inhibition of browning in fresh walnut kernels. Acta Horticulture .1318, 137–140. https://doi.org/10.17660/ActaHortic.2021.1318.20
Hartmann HT, Kester DE, Davies FTJR, Geneve LR (2002) Plant Propagation: Principles and Practices. Seventh Edition. Regents / Prentice Hall International Editions, Englewood Cliffs, New Jersey. pp. 880.
Khalil-ur-Rhaman NA, Khan A, Bakht J, Alam R (2009) Response of various indigenous walnut genotypes to graft take success. Sarhad Journal of Agriculture. 25(3), 399-403.
Liu J, Magige EA, Fan PZ, Wambulwa MC, Luo YH, Qi HL, Milne, RI (2023) Genetic imprints of grafting in wild iron walnut populations in southwestern China. BMC Plant Biology. 23(1), 423. https://doi.org/10.1186/s12870-023-04428-z
Liu H, Gao Y, Song X, Ma Q, Zhang J, Pei D (2018) A novel rejuvenation approach to induce endohormones and improve rhizogenesis in mature Juglans tree. Plant Methods. 14, 1-14. https://doi.org/10.1186/s13007-018-0280-0
Lou H, Wang F, Zhang J, Wei G, Wei J, Hu H, Zhang Q (2024) JrGA20ox1 transformed rootstocks deliver drought response signals to wild type scions in grafted walnut. Horticulture Research. https://doi.org/10.1093/hr/uhae143
Mng’omba SA, Akinnifesi FK, Sileshi G, Ajayi OC (2010) Rootstock growth and development for increased graft success of mango (Mangifera indica) in the nursery. African Journal of Biotechnology. 9(9). 1317-1324.
Mostakhdemi A, Sadeghi-Majd R, Zadeh Bagheri Najafabad M, Vahdati K (2022) Evaluation of patch budding success in Persian walnut affected by different treatments after budding. International Journal of Fruit Science. 22(1), 495-503. https://doi.org /10.1080/ 15538362.2022.2060894
Nosrati Z, Khadivi-Khub A (2014) Effect of different budding methods and times on grafting success of walnut. Korean Journal of Horticultural Science & Technology. 32(6), 788-793. https://doi.org/10.7235/hort.2014.14002
Ozkan Y, Gumus A (2001) Effects of different applications on grafting under controlled conditions of walnut (Juglans regia L.). Acta Horticiculture. 544, 515–520. https://doi.org/10.17660/ActaHortic.2001.544.71
Rébufa C, Artaud J, Le Dréau Y (2022) Walnut (Juglans regia L.) oil chemical composition depending on variety, locality, extraction process and storage conditions: A comprehensive review. Journal of Food Composition and Analysis. 110, 104534. https://doi.org /10.1016/j .jfca.2022.104534
Ribeiro H, Ribeiro A, Pires R, Cruz J, Cardoso H, Barroso JM, Peixe A (2022) Ex vitro rooting and simultaneous micrografting of the walnut hybrid rootstock ‘Paradox’ (Juglans hindsi× Juglans regia) cl.‘Vlach’. Agronomy, 12(3), 595. https://doi.org/10.3390/agronomy12030595
Rezaee R, Vahdati K (2008) Introducing a simple and efficient procedure for topworking Persian walnut trees. Journal of the American Pomological Society. 62(1), 21-26.
Sadeghi-Majd R, Vahdati K, Roozban MR, Arab M (2019) Exploring combinations of graft cover and grafting method in commercial walnut cultivars. International Journal of Fruit Science. 19(4), 359-371. https://doi.org /10.1080/15538362.2018.1535355
Sadeghi-Majd R, Vahdati K, Roozban, MR, Arab M, Sütyemez M (2022) Optimizing environmental conditions and irrigation regimes can improve grafting success in Persian walnut. Acta Scientiarum Polonorum Hortorum Cultus. 21(2), 43-51. https://doi.org /10.24326/asphc.2022.2.4
Singh L, Awasthi M, Negi P, Negi M (2019) Studies on success rate of grafting methods on walnut (Juglans regia L.) at different time under polyhouse condition. Journal of Pharmacognosy and Phytochemistry. 8(4), 2657-2659.
Song X, Zhou N, Chang Y, Zhang J, Pei D (2024) Spatial and temporal alterations of multiple hormones during the graft union formation process in walnut (Juglans regia). Trees, 1-10. https://doi.org/10.1007/s00468-023-02472-8
Thapa R, Thapa P, Ahamad K, Vahdati K (2021) Effect of grafting methods and dates on the graft take rate of Persian walnut in open field condition. International Journal of Horticultural Science and Technology. 8(2), 133-147. https://doi.org/10.22059/ijhst.2020.311553.401
Vahdati K (2003) Nursery management and grafting of walnut. Khaniran, Tehran, Iran. pp. 113.
Vahdati K, Zareie N (2006) Evaluation of side-stub and hypocotyle grafting efficiency for walnut propagation in Iran. Acta Horticulture. 705, 347–351. https://doi.ir/10.17660/ActaHortic.2005.705.47
Vahdati K, Rezaee R, Grigoorian V, Valizadeh M, Motallebi Azar A (2008) Rooting ability of Persian walnut as affected by seedling vigour in response to stool layering. The Journal of Horticultural Science and Biotechnology. 83(3), 334-338. https://doi.org/10. 1080/14620316.2008.11512388
Vahdati K, Sadeghi-Majd R, Sestras AF, Licea-Moreno RJ, Peixe A, Sestras RE (2022) Clonal Propagation of Walnuts (Juglans spp.): A Review on Evolution from Traditional Techniques to Application of Biotechnology. Plants, 11(22), 3040. https://doi.org/10. 3390/plants11223040