Histomorphology of Kidney and Hematological Indices of Nile tilapia, Oreochromis niloticus on Replacement of Blood Powder Instead of Fish Meal in the Diet
Subject Areas : Journal of Animal BiologyAmal Moshashai 1 , رحیم عبدی 2 , rahim peyghan 3
1 - Department of Marine Biology, Faculty of Marine Science and Oceanography, Khorramshahr University of Marine Science and Technology, Khorramshahr, Iran
2 - Department of Marine Biology, Faculty of Marine Science and Oceanography, Khorramshahr University of Marine Science and Technology, Khorramshahr, Iran
3 - Department of Clinical Sciences and Excellence Center of Warm Water Fish Health and Diseases, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran
Keywords: Histomorphology, Kidney, Blood powder, Blood Powder, Nile tilapia,
Abstract :
The present study aimed to investigate the effect of replacing blood powder with fish powder on the histomorphology of kidney tissue and some blood indices of Nile tilapia. After preparation, the fish were fed with designed diets for 8 weeks, including control, 25, 50, 75, and 100% blood powder. After this period, the fish were anesthetized and blood was collected from the caudal peduncle with a heparin syringe. While opening the abdominal cavity, five-millimeter samples were taken from the kidney. After placing in a 10% buffered formalin fixative solution and undergoing dehydration with alcohol, clarification with xylene, and paraffin blocking, sections were made to a thickness of 4-6 microns with a microtome. They were then stained with hematoxylin-eosin and finally studied with a light microscope. Results showed that in the group receiving blood powder, numerous complications such as hyaline accumulation, epithelial rupture, Bowman's capsule dilation, hyperemia, increased melanomacrophage centers, and glomerular necrosis and tubules were observed. As the percentage of blood powder in the diet increased, so did the severity of the lesions. With increasing the replacement of blood powder instead of fish powder in the diet, the number of red blood cells, the percentage of hematocrit, and the amount of hemoglobin show a decreasing trend. The number of lymphocytes, monocytes, and neutrophils increased with increasing levels of blood powder. There was a significant difference between treatments, especially at high levels (p < 0.05). According to the results of this study, blood powder can be tolerated and replaced up to 75% of the diet for this species.
1. Aanyu, M., Betancor, M.B., Monroig, O. 2020. The effects of combined phytogenics on growth and nutritional physiology of Nile tilapia (Oreochromis niloticus). Aquaculture, 519:734867.
2. Abdelhamed, H., Ibrahim, I., Baumgartner, W., Lawrence, M.L., Karsi, A. 2017. Characterization of histopathological and ultrastructural changes in channel catfish experimentally infected with virulent Aeromonas hydrophila. Frontier Microbiology, 8:1519.
3. Adeniran, A., Adeyemo, O.K., Emikpe, B., Alarape, S. 2017. Organosomatic indices, haematological and histological assessment as biomarkers of health status in feral and cultured Clarias gariepinus. African Journal Biomedical Research, 20(2):189-194.
4. Ahmad Mohamad, S. 2009. Effect of different artificial on growth rate condition and histological structure of Nile tilapia (Oreochromus niloticus ). Research Journal of Fish Hydrobiology, 4(1):29- 34.
5. Ahmed, S.A., Ibrahim, R.E., Farroh, K.Y., Moustafa, A.A., Al-Gabri, N. A., Alkafafy, M., Amer, S.A. 2021. Chitosan vitamin E nanocomposite ameliorates the growth, redox, and immune status of Nile tilapia (Oreochromis niloticus) reared under different stocking densities. Aquaculture, 541:736804.
6. Ale, A., Bacchetta, C., Rossi, A.S., Galdoporpora, J., Desimone, M.F., Fernando, R., Cazenave, J. 2018. Nanosilver toxicity in gills of a neotropical fish: Metal accumulation, oxidative stress, histopathology and other physiological effects. Ecotoxicology Environment Safty, 148:976-984.
7. Amerm, S.A., Ahmedm, S.A., Ibrahimm, R.E., Al-Gabrim, N.A., Osmanm, A., Sitohym, M. 2020. Impact of partial substitution of fish meal by methylated soy protein isolates on the nutritional, immunological, and health aspects of Nile tilapia, Oreochromis niloticus fingerlings. Aquaculture, 518:734871.
8. Amiripour, L., Abdi, R., Movahedini,a A., Sahraian, MR. 2015. Study of liver and intestine tissue structure in orange spotted grouper (Epinephelus coioides) during larval development. Journal of Oceanography, 6(23):87-92.
9. Basir, Z., Peyghan, R. 2016. The process of kidney gradual changes in, Tenualosa ilisha during migration from sea to the river. Journal of Persian Gulf, 7(26): 47-56.
10. Basir, Z., Peyghan, R. 2021. The Effect of Replacement of Poultry by-Product with Fish Meal in the Diet of Nile Tilapia (Oreochromis niloticus) on their Intestine Histology. Journal of Veterinary Research, 76(4):467-475.
11. Besson, M., Feeney, W.E., Moniz, I., Francois, L., Brooker, R.M., Holzer, G., Metian, M., Roux, N., Laudet, V., Lecchini, D. 2020. Anthropogenic stressors impact fish sensory development and survival via thyroid disruption. Nature Communications, 11:1-10.
12. Dastan, V., Abdi, R., Movahedinia, A., Salari-Aliabadi, M.A. 2017. Study of gill and kidney tissue changes in Tenualosa ilisha during migration from sea to the Karun and Bahmanshir rivers. Iranian Fisheries Science Journal, 25(4):53-62.
13. El-Naby, F.S.A., Naiel, MAE., Al-Sagheer, A.A., Negm, S.S. 2019. Dietary chitosan nanoparticles enhance the growth, production performance, and immunity in Oreochromis niloticus. Aquaculture, 501:82-89.
14. Hasanzadeh, S., Abdi, R., Salari- Aliabadi, M.A. Movahedinia, A., Basir, Z. 2018. Comparative histomorphology of esophagus and intestine in two carnivorous and phytoplankton feeder fish of Persian Gulf. Journal of Animal Environment, 10(4):381-388.
15. Hassaan, M.S., Goda, M.A.S., Kumar, V. 2017. Evaluation of the nutritive value of fermented deoiled Physic nut, Jatropha curcas seed meal for Nile tilapia, Oreochromis niloticus fingerlings. Aquaculture Nutrition, 23(3):571-584.
16. Hill, J.C., Alam, M.S., Watanabe, W.O., Carroll, P.M., Seaton, P.J., Bourdelais, A.J. 2019. Replacement of menhaden fish meal by poultry by‐product meal in the diet of juvenile red porgy. North American Journal of Aquaculture, 81(1):81-93.
17. Kabir, K..A., Verdegem, M.C.J., Verreth, J.A.J., Phillips, M.J., Schrama, J.W. 2019. Effect of dietary protein to energy ratio, stocking density and feeding level on performance of Nile tilapia in pond aquaculture. Aquaculture, 511:634200.
18. Khodabakhshian, M., Shirali, S., Abdi, R. 2022. Microscopic survey of Balbiani bodies in Yellowfin seabream (Acanthopagrus latus), Abu Mullet (Planiliza abu), Common carp (Cyprinus carpio) and Benni (Barbus sharpeyi). Journal of Animal Research, 34(4):375-389.
19. Li, Y., Kortner, T.M., Chikwati, E.M., Belghit, I., Lock, E.J., Krogdahl, A. 2020. Total replacement of fish meal with black soldier fly (Hermetia illucens) larvae meal does not compromise the gut health of Atlantic salmon (Salmo salar). Aquaculture, 520:734967.
20. Moallem, Z., Abdi, R., Movahedinia, A., Shirali, S., Salati, A.P. 2015. Gonad histology and gonadosomatic index variations during gonadal development of wild female Tenualosa ilisha. Journal Persian Gulf, 6(19):53-58.
21. Mohamed, M., Abdi, R., Ronagh, M.T., Salari-Aliabadi, M.A., Basir, Z. 2021. Comparative histomorphology of epidermis of head and caudal peduncle in Otolithes ruber, Huso huso and Pangasius hypophthalmus fish. Iranian Journal Aquatic Animal Health, 7(1):10-20.
22. Mohamed, M., Abdi, R., Ronagh, M.T., Salari- Aliabadi, MA., Basir, Z. 2020. Comparative histomorphometry of dorsal, ventral and lateral skin in macroscopy, microscopy and free scale fish. Iranian Veterinary Journal, 16(2):47-53.
23. Moradkhani, A., Abdi, R., Salari-Aliabadi, M.A., Nabavi, S., Basir, Z. 2020. Quantification and description of gut-associated lymphoid tissue in, shabbout, Arabibarbus grypus (actinopterygii: cypriniformes: cyprinidae), in warm and cold season. Acta Ichthyology et Piscatoria, 50(4):423-432. [In Persian]
24. Nochalabadi, A., Morovvati, H., Abdi, R. 2023. Histomorphometry of Liver and some Blood Factors of Nile Tilapia, Oreochromis niloticus Exposed to Different Concentrations of Ammonia. Pollution, 9(3): 1225-1235.
25. Nochalabadi, A., Morovvati, H., Abdi, R. 2023. The Effect of different concentrations of ammonia on histomorphometry of kidney and some blood factors of Nile tilapia, Oreochromis niloticus. Iranian Veterinary Journal, 19(3):64-71.
26. Pourkhadje, M., Abdi R., Zolgharnein, H., Hoseinzade, Sahafi, H., Morovvati, H. 2014. Effects of different salinity on number and area of chloride cells in gill of juvenile grouper (Epinephelus coioides). Iranian Journal of Fisherise Science, 23(2):1-10.
27. Roshanfekr, K., Abdi, R., Salari- Aliabadi, MA., Basir, Z. 2017. The impact of spent mushroom compost and fertilizer on esophagus histological indices of some cultured warm water species. Journal of Animal Biology, 10(1):23-33.
28. Roshanfekr, K., Abdi, R., Salari-Aliabadi, M.A., Basir, Z. 2018. The impact of spent mushroom compost and fertilizer on changes of intestinal tissue of cultured warm water species. Journal of Animal Physiology Development, 4(32):11-25.
29. Wardani, WW., Alimuddin, A., Zairin, M., Setiawati, M., Nuryati, S., Suprayudi, MA., 2020. Evaluation of cysteamine supplementation in red tilapia (Oreochromis sp.) diet: Serum insulin and somatostatin, IGF-1 and GLUT4 genes expression, growth performance, and robustness against stress. Aquaculture, 528:735514.
30. Younis, EM., Abdel-Warith, AA., AL-Asgan, NA. 2012. Hematological and enzymatic responses of Nile tilapia, Oreochromis niloticus, during short and long term sublethal exposure to zinc. African Journal of Biotechnology, 11:4442-4446.
