Antibacterial activities of green tea extracts on five plant pathogenic bacteria
Subject Areas : Biotechnological Journal of Environmental Microbiology
1 - Department of Herbal Medicine, Rasht Branch, Islamic Azad University, Rasht, Iran
Keywords: solvent, green tea, antibacterial, aqueous and alcoholic extracts, biological control,
Abstract :
Tea is one of the medicinal plants that is used all over the world. In the preparation of green tea, the leaves are dried after harvesting and then used, and fermentation is not done. Tea extract has antimicrobial properties, but there is little information about this. The leaves of three clones of 100, seed hybrid and Assam were collected from tea gardens, and then the collected leaves were dried. Three solvents; aqueous, methanolic and ethanolic, were used for extraction. The effect of these extracts on the proliferation of five bacteria Clavibacter michiganensis, Xanthomonas arboricola, Pseudomonas syringae, Dikeya zeae and Pectobacterium carotovorum was investigated by the paper disc method by measuring the non-growth halo. The results showed that the highest dilutions of aqueous, ethanolic and methanolic extracts (50%) were the most effective dilutions on the investigated bacteria. All extracts had the ability to control bacteria. The effects of the extracts on bacteria were different. The extracts obtained from clone 100 showed the highest inhibition rate on the studied bacteria. Among the extracts and clones, the highest amount of inhibition on gram positive bacteria C. michiganensis was by water extract. On average, aqueous extract showed the most effect and ethanol extract showed the least effect on bacteria. D. zeae showed the highest resistance against the extracts. It seems that different factors such as type of solvent, concentration, types of microorganisms are effective in the inhibition rate of green tea extract.
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