Identification of tomato root and crown pathogenic fungi in Marvdasht region, Iran

Subject Areas : دو فصلنامه تحقیقات بیماریهای گیاهی

A. Sharzei ¹ , Sara Heidary ² , F. Raufi ³

1 - دانشگاه آزاد اسلامی واحد مرودشت، گروه بیماری شناسی گیاهی، مرودشت، ایران.
2 - کارشناسی ارشد بیماری شناسی گیاهی، گروه بیماری شناسی گیاهی، دانشکده کشاورزی، دانشگاه شیراز، شیراز، ایران.
3 - کارشناسی ارشد بیماری شناسی گیاهی، گروه بیماری شناسی گیاهی، دانشکده کشاورزی، دانشگاه شیراز، شیراز، ایران.

Received: 2013-11-05 Accepted : 2013-11-05 Published : 2013-09-23

Keywords: Macrophomina phaseolina, Rhizoctonia solani, Fusarium oxysporum, Pythium aphanidermatum, Phoma betae, root and crown rot, tomato, Phytophthora nicotiana,

Abstract :

During the years 2009-2010, a survey was conducted in Marvdasht region (Fars province), to identify the casual agents of root and crown rot in tomato plants. Symptomatic and healthy looking samples collected from different fields were surface sterilized and cultured on potato dextrose agar. Eighty isolates belonging to 17 genera were separated from infected roots. Isolates of Pythium aphanidermatum, Phytophthora nicotiana, Rhizoctonia solani, Macrophomina phaseolina, Phoma betae and Fusarium oxysporum were able to cause root rot on tomato plants in pathogenicity tests. R. solani and P. nicotiana were most (26%) and least (2%) frequently isolated species, respectively. Pathogenicity tests on tomato plants also showed that R. solani and M. phaseolina were most severe pathogens. On tomato plants, this is the first report of M. phaseolina from Iran and P. nicotiana from Fars province.

References:

1. Behdad E. 1982. Diseases of Field Crops in Iran. Neshat Press, Isfahan, Iran. 424 p.


2. Booth C. 1971. The genus Fusarium. CMI, Kew, Surrey, England, 237p.


3. Buttner GP, Fahler B, Marlander B. 2004. Greenhouse and field techniques for testing sugarbeet for resistance to Rhizoctonia root and crown rot. Plant Breeding 123:158-166. 


4. Cormarc MW, Moffatt JE. 1961. Factors influencing storage decay of sugar beet by Phoma and other fungi. Phytopathology 51:3-5.


5.  Cucuzza JD, Watterson, JC, Bernhardt EA. 1992. Foot rot of tomato caused by Fusarium solani in California. Plant Disease 76:101.


6. Ershad D. 1992. Phytophthora species in Iran. Plant Pests and Diseases Reasearch Institute, Tehran, Iran, 217p.


7. Ershad D. 1995. Fungi of Iran. Iranian ministry of agriculture. Tehran, Iran, 874p.


8. Etebarian HR. 1989. Study on Fusarium wilt disease of tomato and its chemical control in Varamin area. Iranian Journal of Agricultural Science 32:1-13.


9. Fasihiani A. 1985. Occurrence of Fusarium wilt of tomato in Hormozgan province. Iranian Journal of Plant Pathology 21: 29-32.


10. Gugel, R. K., S. M. Yitbarek, P. R. Verma, R. A. A. Morrall and Sadasiviah, R. S.. 1987. Etiology of the Rhizoctonia root rot complex of canola in the Peace River region of Alberta. Can. J. Plant Pathol. 9: 119-128..


11. Gacitua S, Valienta CF, Diaz KP, Hernandez JC, Uribe MM, Sanfuentes EV. 2009. Identification and biological characterization of isolates with activity inhibitive against Macrophomina phaseolina (Tass) Goid. Chilean Journal of Agricultural Research. 69: 526-533.


12. Jones JB, Stall RE, Zitter TA. 1991. Compendium of tomato diseases. APS Press, St. Paul, Minnesota, USA, 73p.


13. Jarvis WR, Shoemaker RA. 1978. Taxonomic status of Fusarium oxysporum causing foot and root rot of tomato. Phytopathology 68:1679-1680.


14. Nelson PE, Toussoun TA, Marasas WFO. 1983.Fusarium species: an illustrated manual for identification. PennsylvaniaStateUniversity, University Park and London.193 pp.


15. Ommati F, Ershad J. 2004. Identification of fungal agents of tomato wilting from nurseries and field of Semnan province. Proceedings of 16^th Iranian Plant Protection Congress, University of Tabriz, Iran. p249.


16. Karimi A, Noaparast F. 1985. Occurrence of new diseases in tomato fields of Bushehr and southern shores. Iranian Journal of Plant Pathology 5:35.


17. Kappor IJ. 1988. Fungi involved in tomato wilt syndrome in Delhi, Maharashtra and Tamil Nadu. Indian Phytopathology 41:208-213.


18. Parmeter JR. 1970. Rhizoctonia solani, Biology and Pathology. University of California Press. Berkeley, California, USA. 255 p.


19. Rahimian H. 1989. Anastomosis groups of Rhizoctonia solani causing soil rot of tomato fruit in Mazandaran. Iranian Journal of Plant Pathology 24: 9-11.


20. Rezaee, S., and Alizade, A. 1998. S oybean roo and stem rot caused by Phytophthora sojae in Lorestan province. Iranian Journal of Plant Pathology 34: 122-145.


21. Rivera MC, Wright ER, Lopez MV, Garda D, Barrague MY. 2004. Promotion of growth and control of damping off (Rhizoctonia solani) of greenhouse tomatoes amended with vermicompost. International Journal of Experimental Botany 53: 229-235.


22. Sadravi M, Setayesh Mehr F. 2009. Fungal diseases of tomato in North Khorasan province and the reaction of four commercial cultivars to their pathogens. Iranian Journal of Plant Pathology 44: 355-361.


23. Sneh B, Burpee LL, Ogoshi A. 1991. Identification of Rhizoctonia species. The American Phytopathological Society Press, St.Paul Minnesota. 133 pp.


24. SuttonBC. 1980. The Coelomycetes, Fungi Imperfecti with Pycnida, Acervuli and Stromata. C.M.I., Kew, Surrey, England. 696 p.


25. Vander Plaats-Niterink AJ. 1981. Monograph of the Genus Pythium. Studies in Mycology, No. 21. Centraalbureau Voor Schinmelcultures. Netherlands. 242p.


26. Vawdrey LL, Peterson RA. 1988. Fusarium solani in cause of foot rot of tomatoes in central Queensland. Australasian Plant Pathology. 17:24-25.


27. Viani A, Alizadeh A, Babadoust M, Peighami E. 2008. Investigation on Fusarium diseases of tomatoes in East Azerbaijan. Journal of Agricultural Science and Natural Resources 14: 192.


28. Walker JC.1981. Fusarium wilt of tomato. Monograph no. 6. APS Press. 56 p.


29. Whipps JM, Lumsden DR. 1991. Biological control of Pythium species. Biocontrol Science and Technology 1:75-90.


30. Wolcan SM, Lori GA. 1993. Tomato foot rot caused by Fusarium solani. Review of Plant Pathology 72: 2193.