Suppressiveness of rosemary (Rosmarinus officinalis) and fennel (Foeniculum vulgare) essential oils on Fusarium oxysporum
Subject Areas : دو فصلنامه تحقیقات بیماریهای گیاهیH. Salek Mearaji, 1 , R. Salek Naghdi, 2 , Kh. Tafreshi, 3
1 - دانش آموخته کارشناسی ارشد، گروه زراعت و اصلاح نباتات، دانشکده کشاورزی، دانشگاه ایلام، ایلام، ایران.
2 - دانشجوی کارشناسی ارشد، گروه زراعت و اصلاح نباتات، دانشکده کشاورزی، دانشگاه آزاد اسلامی واحد تاکستان، تاکستان، ایران.
3 - دانشجوی کارشناسی ارشد، گروه باغبانی، رشته گیاهان دارویی، دانشکده کشاورزی، دانشگاه آزاد اسلامی واحد ساوه، ساوه، ایران.
Keywords: Fusarium oxysporum, essential oil, Fennel, Antifungal, Rosemary,
Abstract :
Using medicinal plants essential oils to control or suppress plant diseases has been considered by researchers all over the world. Fusarium oxysporum is one of the more common plant disease agents that affects a broad range of plants. In this study an in vitro experiment was set up to investigate the individual or combined effect of essential oils of rosemary and fennel on control of F. oxysporum. Treatments included five concentrations of essential oil of rosemary and fennel at 0, 25, 50, 75 and 100 ppm, using a completely randomized design (CRD) with five replications for each treatment. PDA plates with the desired concentrations of each essential oil were prepared and F. oxysporum was cultured on them. Results showed that essential oil of rosemary caused greater inhibition to fungal growth than that of fennel at all of the concentrations tested. Combined application of both essential oils (rosemary and fennel) had higher efficacy than individual application of them. Single application of essential oils of rosemary and fennel, at the highest concentration, inhibited fungal growth by 68/7 and 49/2% respectively, but combination of these essential oils completely restricted Fusarium growth (100%). Therefore, it is possible to restrict a pathogen more efficiently by application of lower concentrations of two or more essential oils in combination.
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