Improvement in the biological control of gray mold of apples with mixtures of silicon and yeast isolate and induction of defense responses

Subject Areas : دو فصلنامه تحقیقات بیماریهای گیاهی

اسماعیل Zanguei ¹ , H.R Etebarian ² , نوازاله Sahebani ³

1 - دانش آموخته کارشناسی ارشد گروه گیاهپزشکی، پردیس ابوریحان، دانشگاه تهران، تهران، ایران
2 - استاد گروه گیاهپزشکی، دانشگاه آزاد اسلامی واحد شهر ری
3 - دانشیار گروه گیاهپزشکی، پردیس ابوریحان، دانشگاه تهران، تهران، ایران

Received: 2017-06-10 Accepted : 2017-06-10 Published : 2013-07-23

Keywords: Phenolic compounds, peroxidase, catalase, silicon, Gray mold,

Abstract :

The biocontrol agents Candida membranifuciens (A5), Pichia guilliermondi (A6) alone
and in combination with silicon (Si) were tested for biological control of the gray mold of
apple caused by Botrytis cinerea. Results showed that Si decreased the growth of the yeast
isolates A5 and A6 in vitro. Also silicon inhibited the growth of the pathogen at concentration
above 0/8% w/v Results of storage experiments indicated that, the lesion diameters in treated
fruit with the biocontrol combinations of Si % 0/2 +A5andSi % 0/2+A6 were 3/87 and3/48
mm respectively, at 4 C° and for Si % 0/2 +A5 was 5/23 mm at 20 oC, showing better control
than the other treatments. The combination of Si %0/2 +A5 caused a decrease in catalase
activity 4 days after inoculation with pathogen. This combination increased peroxidase
activity and Phenolic accumulation that reached maximum level 2 days after pathogen
inoculation. These results indicate that using antagonists in combination with silicon may
result in further induction of resistance in apple fruits and improve biological control.

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