Examining the serum pattern of Coxiella burnetii in the clinical samples of employees Slaughterhouses in the cities of Isfahan
Subject Areas : microbiologyFahimeh Nourbakhsh 1 , Hossein Chahardahmasoumi 2 , Mohammad Reza Saebi 3
1 - Doctor of toxicology, expert of vice president of food and drug, Isfahan University of Medical Sciences, Isfahan-Iran.
2 - Doctor of veterinary medicine, expert of deputy of food and drug, Isfahan University of Medical Sciences, Isfahan-Iran.
3 - PhD student of food hygiene, Faculty of veterinary medicine, Islamic Azad University, Shahrekord Branch, Shahrekord, Iran
Keywords: Coxiella burnetii, Q fever, Isfahan slaughterhouses,
Abstract :
Background: Q fever is a newly emerging disease in many countries including Iran. Determining the prevalence of contamination and risk factors makes the importance of infection visible to health officials and the necessary facilities and equipment for control and prevention, as well as research priorities.
Material methods: In this study, which was conducted for one year during 1403 in the slaughterhouses of Isfahan city, 100 serum samples were isolated from the employees of the slaughterhouses in Isfahan, and their information was prepared and adjusted. The results of this study have been presented after analysis.
Results: 100 samples isolated from the workers of the studied slaughterhouses, 33% of the cases were reported as positive. In order to finally confirm the presence of bacteria in these 33% positive samples, a molecular method was used. Among the isolated positive cases, 12 cases were employees of the live livestock department, 20 cases were employees of the slaughterhouse department and 1 case were employees of the administrative department. In the molecular method, the genes of the OMP group were isolated. Among these, the genes of 10 of the analyzed samples had the COM1 gene.
Conclusion: The results of the studies show that the one-step polymerase chain reaction method is not sensitive enough to detect Coxiella burnetii and it is suggested to use the nested polymerase chain reaction method. This method has high speed, accuracy, specificity and sensitivity compared to classical methods.
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