Investigation of biofilm production and production of broad spectrum beta-lactamases by Acinetobacter strains isolated from Kerman medical diagnostic laboratories
Subject Areas : microbiology
fatemeh tavakoli
1
,
farokh rokhbakhshzamin
2
1 - Department of Microbiology, Kerman Branch, Islamic Azad University, Kerman, Iran
2 - Department of Microbiology, Kerman Branch, Islamic Azad University, Kerman, Iran
Keywords: Acinetobacter, Antibiotic resistance, Biofilm,
Abstract :
Acinetobacter is an opportunistic hospital pathogen and causes a wide range of these infections, including pneumonia, bacteremia, urinary tract infection and meningitis. This study was conducted with the aim of investigating antibiotic resistance and biofilm formation potential by Acinetobacter strains. This study was conducted on 27 hospital clinical samples. Biochemical tests were performed on the samples in order to isolate and confirm the genus Acinetobacter. Then differential tests were performed. The antibiotic resistance pattern of the strains was determined by the disk diffusion method for 10 antibiotics, then the ability to form biofilm in these strains was evaluated. The results showed that the bacteria were 98-100% resistant to all antibiotics. The results showed that calcitonin was the most effective antibiotic. The results of Acinetobacter cell surface adhesion index at 600 nm showed that two bacteria had an index of more than 70% and two bacteria had an index of less than 30% and the highest value was related to AC5003 and AC5015 strains. The lowest values were for AC5017 and AC5027. The results of the study of biofilm (glass and plastic) bacterial surface adhesion and biofilm formation on plastic tubes that were in the shaker incubator were higher. This study showed an increasing trend of Acinetobacter antibiotic resistance to the above antibiotics and the spread of biofilm-producing clinical isolates. Therefore, due to the clinical importance of this resistant strain in hospitals, its rapid identification and implementation of infection control programs in different parts of the hospital is necessary.
1- Kazemipour N, Dusane D, Dhakephalkar P, Rokhbakhsh Zamin F. 2011 Biofilm formation by Acinetobacter baumanni strains isolated from urinary tract infection and urinary catheters. FEMS Immunology & Medical Microbiology. 2011; 62:328-338.
2- Wisplinghoff H, Schmitt R, Wöhrmann A, Stefanik D, Seifert H. Resistance to disinfectants in epidemiologically defined clinical isolates of Acinetobacter baumannii. J Hosp Infect 2007; 66(2): 174–110.
3- Karyne R, Curty Lechuga G, Almeida Souza AL, Rangel da Silva Carvalho JP, Simões Villas Bôas MH, De Simone SG. Pan-Drug Resistant Acinetobacter Baumannii, but Not Other Strains, Are Resistant to the Bee Venom Peptide Mellitin. Antibiotics, 2020; 9, 178.
4- Da Silva GJ, Domingues S. Interplay between Colistin Resistance, Virulence and Fitness in Acinetobacter Baumannii. Antibiotics, 2017; 6, 28.
5- World Health Organization (WHO). WHO Publishes List of Bacteria for Which New Antibiotics Are Urgently Needed; WHO: Geneva, Switzerland, 2017.
6- Horrevorts A, Bergman K, Kollée L, Breuker I, Tjernberg I, Dijkshoorn L. Clinical and epidemiological investigations of Acinetobacter genomospecies 3 in a neonatal intensive care unit. J Clin Microbiol. 1995; 33: 1567–1572.
7- Bou G, Cervero G, Domingues MA, Quereda C, Martinez-Beltran J. Charactrization of a basocomial outbreak caused by a multiresistant acinetabacter baumannii strain with a carbapenem –hydrolyzing enzyme: high level carbapenem resistance in A.baumannii is not due solely to yhe presense of b-lactamase. J Clin Microbiol 2000; 38:3299-3305.
8- Mohajeri P, Farahani A, Feizabadi MM, Davoodabadi A, Noroozi B. The Prevalence of ESBL Isolates of Acinetobacter baumannii Using PulsedField Gel Electrophoresis. Zahedan Journal of Research in Medical Sciences. 2013; 16(11):20-23
9- Ahmed MN, Porse A, Sommer MO, Høiby N, Ciofu O. Evolution of antibiotic resistance in biofilm and planktonic Pseudomonas aeruginosa populations exposed to subinhibitory levels of ciprofloxacin. Antimicrobial agents and chemotherapy. 2018 Aug 1;62(8):e00320-18.
10- Vijayakumar S, Rajenderan S, Laishram Sh, Anandan Sh. Biofilm Formation and Motility Depend on the Nature of the Acinetobacter baumannii Clinical Isolates. Front Public Health. 2016; 4: 105, doi,10.3389/fpubh.2016.00105.
11- Gordon NC, Wareham DW. Multidrugresistant Acinetobacter baumannii: Mechanisms of virulence and resistance. Int J Antimicrob agents. 2010; 35: 219-26.
12- Evans SR, Hujer AM, Jiang H, Hill CB, Hujer KM, Mediavilla JR, et al. Informing antibiotic treatment decisions: evaluating rapid molecular diagnostics to identify susceptibility and resistance to carbapenems against Acinetobacter spp. in PRIMERS III. J clin microbiol. 2017 Jan;55(1):134-44.
13- Stobberingh EE, Arends J, Hoogkamp-Korstanje JA, Goessens WH, Visser MR, Buiting AG, et al. Occurrence of extended-spectrum betalactamases (ESBL) in Dutch hospitals. Infection 1999; 27:348-354.
14- Hatami R. The frequency of multidrug-resistance and extensively drug-resistant Acinetobacter baumannii in west of Iran. J Clin Microbiol Infect Dis. 2018;1(1):4-8.
15- Shahcheraghi F, Akbari Shahmirzadi N, Abbas Alipour Bashash M, Jabbari H, Amir Mozafari N. Detection of blaCTX,blaTEM beta-lactamase genes in clinical isolates of acinetobacter spp. from selected Tehran hospitals. Iran J Med Microbiol 2009; 3:1-9.
16- Mohammad Bokaian ,Shahram Shahraki ,JanMohammad Raeisi ,Farzaneh Mohammadzadeh Rostami . Prevalence of OXA-2 and OXA-10 Type ESBL among Acinetobacter Strains Isolated from Patients of Zahedan.Medical journal of Mashhad ,2016;26-34.
17- Qi L, Li H, Zhang C, Liang B, Li J, Wang L, et al. Relationship between antibiotic resistance, biofilm formation and biofilm-specific resistance in Acinetobacter baumannii. Front Microbiol. 2016; 7:483
18- Vahid Rouhi , Roya Safarkar1, Sanaz Habibi. Phonotypic Investigation of Biofilm Formation and Determination of Presence of bap and blaOXA-51 Genes in Acinetobacter baumannii From Clinical Specimens in Tehran. Iran J Med Microbiol. 2020; 14(6): 566-583
19- Lihua Q, Hao L, Chuanfu Zh, Beibei L, Jei L. Relationship between Antibiotic Resistance, Biofilm Formation, and Biofilm-Specific Resistance in Acinetobacter baumannii,Front Microbiology, 2016; 7: 483, https://doi.org/10.3389/fmicb.2016.00483
20- Teymori Fatemeh, AmirMozafari sabet noutr. Studing the realationship between the ability of biofilm formation and antibiotic resistance in Acentobacter Baumannii clinical and envaromental isolates in Tehran. Arak Medical University Journal(AMUJ).2017
21- Vijayakumar S, Rajenderan S, Laishram Sh, Anandan Sh. Biofilm Formation and Motility Depend on the Nature of the Acinetobacter baumannii Clinical Isolates. Front Public Health. 2016; 4: 105, doi,10.3389/fpubh.2016.00105.
