Characterization of pharmacological properties and isolation of two bioactive compounds (ursolic acid and palmitoleic acid) from the stem bark extract of Lannea coromandelica

Subject Areas : Phytochemistry: Isolation, Purification, Characterization

Ramisa Anjum ¹ ()
A.H.M. Nazmul Hasan ² ()
Mst. Al Asma Hawa ³ ()
Md. Naimur Rahman ⁴ ()
Md. Khokon Miah Akanda ⁵ ()
Tania Binte Wahed ⁶ ()
Fayad Bin Abdus Salam ⁷ ()
Md. Rabiul Islam ⁸ ()
Mohammad Shahriar ⁹ ()

Received: 2024-01-13 Accepted : 2024-06-15 Published : 2024-06-28

Keywords: Antidiabetic, Antioxidants, Bioactive compound isolation, Lanna coromendelica, Palmitoleic acid, Ursolic acid,

Abstract :

The current research aimed to conduct a bioassay-guided isolation of bioactive compounds from the stem bark of Lannea coromandelica. Using column chromatography and NMR spectroscopy, two compounds -ursolic acid and palmitoleic acid- were isolated from the plant’s stem bark. The methanolic stem bark extract possessed higher total phenolic, flavonoid, and antioxidant capacity content than the n-hexane extract. The IC50 value of the methanol and n-hexane stem bark extracts for DPPH free radical scavenging potential was found to be 37.37 and 27.726 μg/mL, respectively, while for nitric oxide, the IC50 value was 14.615 and 22.136 μg/mL, respectively. The n-hexane extract exhibited higher antidiabetic effectiveness (46% blood glucose reduction) than the methanolic extract (37.8% blood glucose reduction) after 3 hours of glucose administration in mice model at a dose of 400 mg/kg body weight (p < 0.001). The methanolic extract demonstrated the highest anti-diarrheal action at a dose of 400 mg/kg body weight (88.70% inhibition of defecation, p < 0.001). Furthermore, the n-hexane extract showed the highest analgesic activity (70.5% inhibition of writhing, p < 0.01).

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