Effect of intensity, duration and power of ultrasonic waves on germination indices and photosynthetic pigments of canola seedling
Subject Areas :
Heshmat
Omidi
1
(Agronomy and Plant Breeding Department, Faculty of Agricultural Sciences, Shahed University, Tehran, Iran)
Saba
Dashab
2
(Agronomy and Plant Breeding Department, Faculty of Agricultural Sciences, Shahed University, Tehran, Iran)
Keywords: chlorophyll content, seed priming, carotenoid content, Nanopriming,
Abstract :
Seed priming using biophysical methods including ultrasonic wave causes changes in germination and some physiological traits of seedling. Accordingly, in order to investigate the effect of intensity and duration of ultrasonic wave on germination of rapeseed cv. Modena, an experiment as factorial was conducted based on a completely randomized design with three replications in Laboratory condition. Experimental factors consisted of power 60, 80, and 100 W and two ultrasonic waves 40 and 59 kHz at duration of 2, 4, 6, 8, and 20 minutes. The triple interaction of power × irradiation intensity × irradiation time was significant on germination indices including percentage and rate of germination, seedling vigor index of length and weight and seedling traits including chlorophyll a, b and total, and carotenoid content. Germination percentage using 40 and 59 kHz ultrasound waves at 100 W for 2 minutes was increased by 17.8%. The seedling vigor index of length and weight were improved using 59 kHz ultrasonic power with 100 W, and compared to control treatment had increasing 33.8 and 25.8%, respectively. In the application of 40 kHz ultrasound power with 80 W in 8 minutes, total chlorophyll, chlorophyll a, and chlorophyll b content than control treatment was 61.26, 26.3, and 34.9 µg/g FW, respectively. Based on the present research, seed pre-treatment of 40 and 59 kHz ultrasound waves with 100 watts can be recommended to improve seed germination indices and increase photosynthetic pigments contents.
_||_
