Investigation of myeloperoxidase activity, nitric oxide and vitamin C level in the serum of vaccinated and brucellosis-infected cattle and sheep

Subject Areas : Veterinary Clinical Pathology

majid shafie ¹ , Gholamali Kojouri ² , behnaz karimi babaahmadi ³ , Hooman kojouri ⁴

1 - Graduate of Veterinary Medicine, Faculty of Veterinary Medicine, Shahrekord University, Shahrekord, Iran.
2 - Professor, Department of Clinical Sciences, Faculty of Veterinary Medicine, Shahrekord University, Shahrekord, Iran.
3 - Assistant Professor, Department of Basic Sciences, Faculty of Veterinary Medicine, Shahrekord University, Shahrekord, Iran.
4 - Post Graduate Student of Pediatrics, Faculty of Medicine, Iran University of Medical Sciences, Tehran, Iran.

Received: 2024-06-26 Accepted : 2024-12-11 Published : 2025-05-20

Keywords: Brucellosis cow and sheep, Myeloperoxidase, Nitric oxide, Vaccination, Vitamin C,

Abstract :

Brucellosis is a zoonotic infectious disease that causes significant economic losses. This infection damages the oxidant and antioxidant balance of the body. This research was conducted to evaluate and compare the activity of myeloperoxidase (MPO), nitric oxide, and vitamin C concentration in vaccinated and brucellosis-infected cattle and sheep. For this purpose, 40 blood samples consisting of 5 ml were obtained from the jugular vein of vaccinated and brucellosis-infected cattle and sheep (10 samples from each group) and the activity of the MPO enzyme and the levels of nitric oxide and vitamin C in the serum of the animals were measured and compared. A significant increase in MPO enzyme activity was observed in brucellosis-infected cattle and sheep compared to vaccinated animals, which was associated with increased neutrophil recruitment and enhanced inflammatory response (p<0.05). The nitric oxide levels were found to be significantly higher in infected cows compared to vaccinated cows, which is probably related to macrophage-stimulating nitric oxide production by bacterial lipopolysaccharide (p<0.05). However, no significant difference was observed between vaccinated and infected sheep. Also, the vitamin C levels were significantly higher in vaccinated cattle than in infected cattle (p<0.05). The findings of the current research indicated that, probably due to brucellosis, the oxidative activity in the serum increases, while vaccination against brucellosis plays an important role in the body's antioxidant resistance. Although, it seems that, more extensive research and evaluation of other contributing factors are needed to make a definitive opinion in this regard.