Investigation of serum myeloperoxidase activity, nitric oxide and vitamin C concentration in vaccinated and brucellosis-infected cows and sheep in Shahrekord district

majid shafie ¹ ( Graduate Student, Faculty of Veterinary Medicine, Shahrekord University, Shahrekord, Iran )
Gholamali Kojouri ² ( Professor, Department of Clinical Sciences, Faculty of Veterinary Medicine, Shahrekord University, Shahrekord, Iran. )
behnaz karimi babaahmadi ³ ( Department of Basic Science, Faculty of Veterinary Medicine, Shahrekord University, shahrekord, Iran )
Hooman kojouri ⁴ ( Student of pediatrics residency, Faculty of Medicine, Iran University of Medical Sciences, Tehran, Iran. )

Submited date : 2024-06-20 Accepted date : 2025-03-02

Keywords: Brucellosis, myeloperoxidase, nitric oxide, vitamin C, Brucellosis vaccine,

Abstract :

Brucellosis is a common disease between wild and domestic animals that causes significant economic losses. This research was conducted to investigate the serum myeloperoxidase (MPO) activity, nitric oxide, and vitamin C concentration in vaccinated and infected cases of cows and sheep. A total of 40 blood samples were obtained from both mentioned groups and the results were statistically analyzed. The results indicated a significant increase in MPO activity in brucellosis-infected cows and sheep compared to the vaccinated animals (p<0.05). The NO levels were found to be significantly higher in infected cows compared to vaccinated cows, which is probably related to macrophage-stimulating nitric oxide production by bacterial lipopolysaccharide. Also, the vitamin C levels were significantly higher in vaccinated cows than in infected cows. This difference may be attributed to the role of vitamin C in acquired immunity after vaccination, fighting diseases, and increasing the uptake of its precursor molecule, glucose, in brucellosis. The results show that the oxidant-antioxidant balance is disturbed in Brucellosis and this is one of the major factors contributing to the formation the disease. Also, the increase in the serum level of MPO enzyme as an indirect indicator of neutrophil influx in livestock brucellosis were reported for the first time.

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