Molecular detection of bacteria causing diarrhea in children with diarrhea in Shahrekord city
Subject Areas : microbiologyZeynab Marzat 1 , Hossein Khodabandeh shahraki 2 , Mohammad Rajabi 3 , payam razeghi tehrani 4
1 - 1. Department of Microbiology, Faculty of Basic Siences, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran
2 -
3 - 1. Department of Microbiology, Faculty of Basic Siences, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran
4 - عضوهیات علمی دانشگاه
Keywords: Bacteria, Children, Diarrhea, Molecular detection, Shahrekord,
Abstract :
Diarrhea is one of the most prevalent diseases in the children especially in the developing countries and is considered as the second cause of mortality following respiratory infections. It is estimated that 3-5 million cases of this disease result in death each year. Mortality rate especially in the children under 5 is significant. In this study 60 diarrhea samples of children who referred at Hajar and Imamali Hospitals of Shahrekord, at the present of specific primers for common bacterial pathogens such as E. coli, C .jejuni, C. coli, S Enteritidis and Y. enterocolitica, were tested. The most abundant studied gene was related to 16srRNA E.coli, so that it was identified in 15 samples (25%). Out of 15 studied cases diagnosed as positive in respect of 16srRNA gene sequence, ipaH gene related to E.coli bacteria resulting in diarrhea was observed in 2 samples (13.33%). hipo gene sequence related to C. jejuni was observed in 1sample and asp gene sequence related to C. coli wasn't observed in any samples. Redundancy of ail gene related to Information about the prevalence of diarrhea resulting bacteria can be effective on correct control and managing the diarrhea in the infants and children. Then it is necessary to do wide research in various seasons of the year and also in all towns of Chharmahal and Bakhtiary province.
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