Extraction of Carotenoids from Ulva rigida by Ultrasound-assisted Method

Subject Areas : Chem

Marzieh Ahmadi-Sarkhoni ¹ ( Department of Food Science and Technology, Faculty of Agriculture, Isfahan (Khorasgan) Branch, Islamic Azad University, Isfahan, Iran )
Mahshid Jahadi ² ( Department of Food Industry Science and Engineering, Faculty of Agriculture and Natural Resources, Isfahan Branch (Khorasgan), Islamic Azad University, Isfahan, Iran )

Received: 2024-05-12 Accepted : 2024-09-06 Published : 2024-09-25

Keywords: Carotenoids, Optimization, Ultrasound-assisted Method, Ulva rigida ,

Abstract :

Background and Objectives: Ulva rigida is a type of green algae, belonging to the chlorophyta; which is known as a source of carotenoid pigments and chlorophyll due to its numerous chemical compounds. In this research, the extraction of carotenoid pigment was optimized using ultrasound-assisted method by response surface methodology. Materials and Methods: green algae Ulva rigida; In May 2022, fresh from the north of Qeshm Island, Direstan coast (Deirestan Gulf), the boundary of the region; The long slope up to Nakhzah and Salakh harbors, with latitude 260732329 and longitude 550848291, was taken. in order to; Optimizing the conditions of carotenoid extraction, using the response surface methodology, with three independent variables; ultrasonic power (5-300 W), ultrasonic time (1.59-18.40 minutes) and biomass-to-solvent ratio (1.63-8.46%). Findings: Optimizing carotenoid extraction conditions using ultrasound-assisted method showed that All independent variables had a significant effect on the efficiency of carotenoids extraction (p<0.05). In the optimum condition of carotenoid extraction using ultrasound-assisted method (ratio of biomass to solvent is 5.57%; ultrasonic power 177.38 W and ultrasonic time 9.93 min), carotenoid content and extraction efficiency were 0.99 ± 0.08 µg/ml and 18.03% respectively. Conclusion: The content and the extraction efficiency of carotenoid using ultrasound-assisted technology increased 4 times compared to the conventional extraction method. Therefore, using ultrasound-assisted method for extraction of carotenoids from Ulva rigida were considered as an effective process.

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