Exploration of metabolic variations, anti-cholinesterase, anti-heme biocrystallization, and anti-protein denaturation activities of ten Capsicum accessions under different stages of ripening

Subject Areas : Medicinal and Herbal Plants

Mamita  Debnath ¹ (Phytochemistry and Pharmacognosy Laboratory, Department of Botany, Ballygunge Science College, 35, Ballygunge Circular Road, Kolkata-700019, West Bengal, India)
Jhelam   Chatterjee ² (Phytochemistry and Pharmacognosy Laboratory, Department of Botany, Ballygunge Science College, 35, Ballygunge Circular Road, Kolkata-700019, West Bengal, India)
Susmita  Das ³ ( Phytochemistry and Pharmacognosy Laboratory, Department of Botany, Ballygunge Science College, 35, Ballygunge Circular Road, Kolkata-700019, West Bengal, India)

Received: 2023-09-27 Accepted : 2024-03-01 Published : 2024-03-05

Keywords: Anti-cholinesterase, Anti-heme biocrystallization, Capsicum species, Cultivars, Ripening stages,

Abstract :

Hydro-methanolic extracts of pericarps and seeds of 10 different Capsicum cultivars from three different species viz., Capsicum chinense, C. annuum and C. frutescens were assessed to determine metabolic variations and anti-cholinesterase, anti-malarial and anti-protein denaturation activities at four different stages of ripening, namely green unripe (GU), turning green-orange (TGO), orange ripe (OR) and red fully ripe (RFR) stages. Many of the ripening stages of the tested cultivars showed significant bioactivities in a dose-dependent manner. In addition, metabolites were identified using GC/MS. On the other hand, HPLC analysis revealed the highest capsaicin and dihydrocapsaicin content in the pericarp of GU, TGO and RFR stages of C. chinense. β-Carotene, capsanthin and lutein contents of C. chinense were also measured using HPTLC technique. The GU and RFR stages of C. chinense contained appropriate amounts of all three carotenoids. These results may be helpful in selecting the cultivars with the best attributes.

References:

Agrawal, P., Jain, M., 2023. A review of selected herbs responsible for wound management. Trends Phytochem. Res. 7(3), 170-185.
Aldulaimi, O., Uche, F.I., Hameed, H., Mbye, H., Ullah, I., Drijfhout, F., Claridge, T.D.W., Horrocks, P., Li, W.W., 2017. A characterization of the antimalarial activity of the bark of Cylicodiscus gabunensis Harms. J. Ethnopharmacol. 198, 221-225.
Ali, H., Alli, I., Ismail, A., Kermasha, S., 2012. Protein-phenolic interactions in food. Eurasian J. Anal. Chem. 7, 123-133.
Altemimi, A., Lakhssassi, N., Baharlouei, A., Watson, D.G., Lightfoot, D.A., 2017. Phytochemicals: Extraction, isolation, and identification of bioactive compounds from plant extracts. Plants (Basel). 6(4), 42.
Bosland, P., Baral, J., 2007. ‘BhutJolokia’ — The world’s hottest known chile pepper is a putative naturally occurring interspecific hybrid. HortScience 42(2), 222-224.
Bosland, P.W., 1996. Capsicums: Innovative Uses of An Ancient Crop. In: J. Janick (ed.), Progress in New Crops. ASHS Press, Arlington, VA.
Bosland, P.W., Votava, E.J., 2012. Peppers: Vegetable and Spice Capsicums. CABI Publishing, Wallingford, UK.
Castillo, E., Lopez-Gonzalez, I., De Regil-Hernandez, R., Reyes-Duarte, D., Sánchez-Herrera, D., López-Munguía, A., Darszon, A., 2007. Enzymatic synthesis of capsaicin analogs and their effect on the T-type Ca2+ channels. Biochem. Biophys. Res. Commun. 356(2), 424-430.
Chen, L., Yu, J., 2016. Modulation of Toll-like receptor signaling in innate immunity by natural products. Int. Immunopharmacol. 37, 65-70.
Chen, S., Pang, X., Song, J., Shi, L., Yao, H., Han, J., Leon, C., 2014. A renaissance in herbal medicine identification: from morphology to DNA. Biotechnol. Adv. 32(7), 1237-1244.
Choi, Y., Ban, I., Lee, H., Baik, M.Y., Kim, W., 2019. Puffing as a novel process to enhance the antioxidant and anti-inflammatory properties of Curcuma longa L. (turmeric). Antioxidants 8(11), 506.
Chung M-K., Campbell, J.N., 2016. Use of capsaicin to treat pain: Mechanistic and therapeutic considerations. Pharmaceuticals 9(4), 66.
Cichewicz, R.H., Thorpe, P.A., 1996. The antimicrobial properties of chilli peppers (Capsicum species) and their uses in Mayan medicine. J. Ethnopharmacol. 52(2), 61-70.
Das, M., Basu, S., Banerjee, B., Sen, A., Jana, K., Datta, G., 2018. Hepatoprotective effects of green Capsicum annum against ethanol induced oxidative stress, inflammation and apoptosis in rats. J. Ethnopharmacol. 227, 69-81.
Das, S., Dutta, M., Chaudhury, K., De, B., 2016. Metabolomic and chemometric study of Achras sapota L. fruit extracts for identification of metabolites contributing to the inhibition of α-amylase and α-glucosidase. Eur. Food Res. Technol. 242, 733-743.
Das, S., Gupta, P., De B., 2017. Thin layer chromatographic characterization of carotenoid isolates in sugar date palm (Phoenix sylvestris) fruit epicarp and inflorescence axis. Int. J. Pharmacogn. Phytochem. Res. 9, 680-684.
Debnath, M., Das, S., Bhowmick, S., Karak, S., Saha, A., De, B., 2021. Anti-Alzheimer’s potential of different varieties of Piper betle leaves and molecular docking analyses of metabolites. Free Radic. Antioxid. 11(1), 13-18.
Devi, P., Kumar, P., 2023. Herbal Medicine and Pregnancy. In “Herbal Medicine Phytochemistry: Applications and Trends (pp. 1-31).”, Cham: Springer International Publishing.
Dogan, A.N.C., Celik, E., Kilicle P.A., Atalay, E., Saglam, A.G., Dogan, A., Otlu, S., 2018. Antibacterial effect of hot peppers (Capsicum annuum, Capsicum annuum var globriusculum, Capsicum frutescens) on some arcobacter, campylobacter and helicobacter species. Pak. Vet. J. 38(3), 266-270.
Ellman, G.L., Courtney, K.D., Andres, V., Featherstone, R.M., 1961. A new and rapid colorimetric determination of acetylcholinesterase activity. Biochem. Pharmacol. 7(2), 88-95.
Fordjour, P.A., Adjimani, J.P., Asare, B. Duah-Quashie, N.O., Quashie, N.B., 2020. Anti-malarial activity of phenolic acids is structurally related. Malarial J. (BMC), doi: 10.21203/rs.3.rs-21702/v1.
Ganie, S.H., Upadhyay, P., Das, S., Sharma, M.P., 2015. Authentication of medicinal plants by DNA markers. Plant Gene, 4, 83-99.
Graefe, S.B., Rahimi, N., Mohiuddin, S.S., 2023. Biochemistry, Substance P. In Stat Pearls. Stat Pearls Publishing.
Hernández-Ortega, M., Ortiz-Moreno, A., Hernández-Navarro, M., Chamorro, G., Dorantes, L., Necoechea-Mondragón, H., 2012. Antioxidant, antinociceptive, and anti-inflammatory effects of carotenoids extracted from dried pepper (Capsicum annuum L.). J. Biotechnol. Biomed., doi: org/10.1155/2012/524019.
Hussain, A.I., Anwar, F., Rasheed, S., Nigam, P.S., Janneh, O., Sarker, S.D., 2011. Composition, antioxidant and chemotherapeutic properties of the essential oils from two Origanum species growing in Pakistan. Rev. Bras. Farmacogn. 21, 943-952.
Idris, O.A., Wintola, O.A., Afolayan, A.J., 2017. Phytochemical and antioxidant activities of Rumex crispus L. in treatment of gastrointestinal helminthes in Eastern Cape Province, South Africa. Asian Pac. J. Trop. Biomed. 7(12), 1071-1078.
Ilkay, O., Kartal, M., Tosun, F., Bilge, S., 2007. Screening of various phenolic acids and flavonoid derivatives for their anticholinesterase potential. Z. Naturforsch., C, J. Biosci. 62(11-12), 829-832.
Jolayemi, A.T., Ojewole, J.O.A., 2013. Comparative anti-inflammatory properties of capsaicin and ethyl-acetate extract of Capsicum frutescens linn [Solanaceae] in rats. Afr. Health Sci.13(2), 357-361.
Kind, T., Wohlgemuth, G., Lee, D.Y., Lu, Y., Palazoglu, M., Shahbaz, S., Fiehn, O., 2009. FiehnLib-mass spectral and retention index libraries for metabolomics based on quadrupole and time-of-flight gas chromatography/mass spectrometry. Anal. Chem. 81(24), 10038-10048.
Loizzo, M.R., Tundis, R., Menichini, F., Statti, G.A., Menichini, F., 2008. Influence of ripening stage on health benefits properties of Capsicum annuum var. acuminatum L.: In vitro studies. J. Med. Food 11(1), 184-189.
Maokam, C., Techawongstien, S., Chanthai, S., 2014. Determination of major and minor capsaicinoids in different varieties of the Capsicum fruits using GC-MS and their inhibition effect of the chilli extract on α-amylase activity. Int. Food Res. J. 21(6), 2237-2243.
Mehta, I., 2017. Chillies-The prime spice – A history. IOSR-JHSS 22(7), 32-36.
Mizushima, Y., Kobayashi, M., 1968. Interaction of anti-inflammatory drugs with serum proteins, especially with some biologically active proteins. J. Pharm. Pharmacol. 20(3), 169-173.
Mohammadhosseini, M., Frezza, C., Venditti, A., Akbarzadeh, A., 2019a. Ethnobotany and phytochemistry of the genus Eremostachys Bunge. Curr. Org. Chem. 23, 1828-1842.
Mohammadhosseini, M., Venditti, A., Akbarzadeh, A., 2019b. The genus Perovskia Kar.: Ethnobotany, chemotaxonomy and phytochemistry: A review. Toxin Rev. 40(4), 484-505.
Nath, D.R., Bhuyan, M., Goswami, S., 2006. Botanicals as mosquito larvicides. Def. Sci. J. 56(4), 507-511.
Ombugadu, A., Micah, E.M., Adejoh, V.A., Odey, S.A., Njila, H.L., Nkup, C.D., Pam, V.A., Echor, B.O., Pam, D.D., Ahmed, H.O., Aimankhu, O.S., Deme, G.G., Uzoigwe, N.R.,Yohanna, J.A., Samuel, M.D., Lapang, M.P., Dogo, K.S., Attah, S.A., Aliyu, A.A., Ayuba, S.O., Musa, M., Angbalaga, G.A., Mafuyai, M.J., 2020. Capsicum chinensis (hot pepper) powder larvicidal activity against mosquitoe larvae in Lafia Local Government Area, Nasarawa State, Nigeria. Biomed J. Sci. Tech. Res. 31(5), 24507-24511.
Palevitch, D., Craker, L.E., 2012. Nutritional and medical importance of red pepper (Capsicum spp.). J. Herbs Spices Med. Plants 3(2), 55-83.
Pawar, S.S, Bharude, N.V., Sonone, S.S., Deshmukh, R.S., Raut, A.K., Umarkar, A.R., 2011. Chillies as food, spice and medicine: A perspective. Int. J. Pharm. Biol. Sci. 1(3), 311-318.
Sakat, S., Juvekar, A.R., Gambhire, M.N., 2010. In vitro antioxidant and anti-inflammatory activity of methanol extract of Oxalis corniculata Linn. Int. J. Pharm. Pharm. Sci. 2(1), 146-155.
Sanati, S., Razavi, B.M., Hosseinzadeh, H., 2018. A review of the effects of Capsicum annuum L. and its constituent, capsaicin, in metabolic syndrome. Iran J. Basic Med. Sci. 21(5), 439-448.
Sharifi-Rad, J., Herrera-Bravo, J., Semwal, P., Painuli, S., Badoni, H., Ezzat, S.M., Farid, M.M., Merghany, R.M., Aborehab, N.M., Salem, M.A., Sen, S., 2022. Artemisia spp.: An update on its chemical composition, pharmacological and toxicological profiles. Oxid. Med. Cell. Longev. doi: org/10.1155/2022/5628601.
Singh, D., Mittal, N., Siddiqui, M.H., 2023. A review on pharmacological potentials of phenolic diterpenes carnosic acid and carnosol obtained from Rosmarinus officinalis L. and modern extraction methods implicated in their recovery. Trends Phytochem. Res. 7(3), 156-169.
Unuofin, J.O., Otunola, G.A., Afolayan, A.J., 2017. Phytochemical screening and in vitro evaluation of antioxidant and antimicrobial activities of Kedrostis Africana (L.) Cogn. Asian Pac. J. Trop. Biomed. 7(10), 901-908.
Usman, M.G., Rafii, M.Y., Ismail, M.R., Malek, M.A., Latif, M.A., 2014. Capsaicin and dihydrocapsaicin determination in chilli pepper genotypes using ultra-fast liquid chromatography. Molecules 19(5), 6474-6488.
Villa-Rivera, M.G., Ochoa-Alejo, N., 2020. Chili pepper carotenoids: Nutraceutical properties and mechanism so faction. Molecules 25(23), 5573.
Waite, M.S., Aubin, A.J., 2008. A Modular HPLC System for Routine Analysis of Capsaicin from Hot Sauces. Waters Corporation, Milford, MA, U.S.A: 5.