Genetic diversity of different ecotypes of ( E. amoneum and B. officinalis ) borage flower species based on essence composition and grain oil rate

Subject Areas : crop production

سيما اوجاني ¹

1 - كارشناسي ارشد گروه كشاورزي – گياهان دارويي واحد ابهر، دانشگاه آزاد اسلامي، ابهر – ايران

Received: 2017-11-03 Accepted : 2018-04-10 Published : 2018-05-31

Keywords: borage , chromatography , ecotype , essential oil , grain oil , linoleic acid,

Abstract :

To determine genetic diversity, oil essence, physical and chemical properties, total grain oil content and fatty acid composition, an experiment was carried out in agricultural research center of W. Azarbaijan province. Plant materials included three ecotypes of Iranian borage flower ( E. amoneum ) from Gazvin, Sari and Gilan and European borage flower ( B. officinalis ) collected from Karaj and Ardabil. The results of analyses of variance and mean comparison showed that there was high genetic diversity among ecotypes for all of traits. The oil essence was extracted by water-distillation from flower organs of each ecotype. Analysis of oil essence with pentane 0.05% mass-gas chromatography showed that E. amoneum had the highest caryophyllene- α and calacorene-α ( 6.3% and 6.5% in Gilan ecotype,) respectively than B. officinalis. The highest levels of n-Decane and Dodecane were found in 7.3% and 7.7% of B. officinalis from Karaj ecotype and Amorphene-α with 6.6% from Ardabil ecotype. Grain oil was extracted by Soxhlet and its compounds were identified by gas chromatography. Fourteen fatty acid was detected in composition. The steeridonic fatty acid (o.o1% ) and linoleic fatty acid ( 32% ) had the lowest and the highest values in both ecotypes of Karaj and Ardabil, respectively. Keywords:

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