Isoleucine and aerobic training regulated the hepatic metabolism in obese mice.

Subject Areas : Exercise Physiology and Performance

Farzad Forootan ¹ , Fatemeh Hajibabaie ² , Zahra Ahmadi ³

1 - Legal Medicine Research Center, Legal Medicine Organization, Tehran, Iran.
2 - Department of Biology, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran.
3 - Department of Physiology, Medicinal Plants Research Center, Isfahan (Khorasgan) Branch, Islamic Azad University, Isfahan, Iran.

Received: 2022-11-10 Accepted : 2023-02-13 Published : 2023-04-01

Keywords: liver, isoleucine, Aerobic exercise, Ppar-γ, Pgc1, Ucp2,

Abstract :

Background: Obesity harmfully affects all physiological functions of the body and public health. Valine(Valine) are crucial amino acids, including leucine, isoleucine, and valine. Isoleucine could influence protein metabolism, apoptosis, regeneration of hepatocytes, and insulin resistance. Previous studies demonstrated that elevated circulating isoleucine are strongly associated with metabolic disorders, such as obesity, metabolic syndrome, and type 2 diabetes mellitus. This study examines the effect of Isoleucine supplements on hepatic lipogenesis and obesity following an acute bout of exercise in adult mice.Methods: Thirty male mice of type C57BL/6 were distributed randomly into five groups: Regular diet group, High-fat diet group, Exercise group, Regular diet+20% Isoleucine diet group, and 20% Isoleucine diet+Exercise group. Next, each group was sacrificed and then, the liver was collected; Real-time qPCR investigated the expression of mRNA levels.Results: Our data indicated that the Exercise group, 20%VALINE, and 20% VALINE+Exercise groups, significantly amplified the levels of Ucp2, Ppar-γ, Ppar-α, and Pgc1-α mRNA compared to the control group. In contrast, the expression level of Ppar-α in the high-fat diet group compared to the control group was decreased.Conclusion: Interestingly, a high-fat diet was due to down-regulated expression levels of Pgc1-α, Ppar-γ, and Ucp2 in the liver, but Ppar-α increased.

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