Evaluation of Lung Surfactant on Gene Expression of MCP-1 in Peripheral Blood Mononuclear Cells in Rabbit Model
Subject Areas : Journal of Animal BiologyM. Aliverdilo 1 , M.R. Mokhber Dezfouli 2 , Z. Eftekhari 3 , M. Paryan 4
1 - Departement of Genetics, Tehran Medical Science Branch, Islamic Azad University, Tehran, Iran
2 - Department of Clinical Sciences, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
3 - Research and Production Complex, Pasteur Institute of Iran, Karaj, Iran
4 - Research and Production Complex, Pasteur Institute of Iran, Karaj, Iran
Keywords: Gene expression, Rabbit, MCP-1, Lung surfactant,
Abstract :
Exogenous lung surfactant has been prescribed in some respiratory disorders. Monocyte chemotactic protein-1 produced by monocytes induces phagocytosis to remove infection from the body. For in vivo evaluation, five rabbits were selected, anesthetized and surfactant solutions were infused into the lungs. Blood samples were collected to determine the cytokines at 0, 24, 48 and 72 h, 7 d and 30 d after drug administration and gene expression evaluated by Real-time PCR. Serum levels of MCP-1 increased at 24 h after surfactant therapy. Additionally, gene expression of MCP-1 at 48 h after drug administration reached its maximum. A surfactant as a therapeutic agent can up-regulates cytokines release from cells in different ways and could contribute to the recruitment of inflammatory cells within the air spaces in different condition.
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