The Effect of Hydroalcoholic Extract of Passiflora caerulea Leaf on Some Biochemical Factors in Adult Male Rat

Subject Areas : Journal of Animal Biology

T. Sadeghi ¹ ( Department of Biology, Kazerourn Branch, Islamic Azad University, Kazerourn, Iran )
M. Shariati ² ( Department of Biology, Kazerourn Branch, Islamic Azad University, Kazerourn, Iran )
M. Mokhtari ³ ( Department of Biology, Kazerourn Branch, Islamic Azad University, Kazerourn, Iran )

Received: 2018-01-21 Accepted : 2018-06-10 Published : 2018-08-23

Keywords: Urea nitrogen, total protein, albumin, Creatinine, Passiflora Caerulea,

Abstract :

Passiflora caerulea is one of medicinal plants that has contained high anti-oxidant combinations and are useful in curing epilepsy, diarrhea, burn, Hemorrhoid and in regulating liver enzymes. The objective of this study was evaluation of effect of passiflora caerulea leaf hydroalcoholic extract on some biochemical factors in adult male rat. 40 male rats, about 195-200 g each were used in five groups. The control group did not receive any drug treatment, sham group found only distilled water, and experimental groups receiving 150,300,600, mg/kg leaves of passiflora caerulea Hydro-alcohol extract respectively during 21 days. After the end of this period, blood samples were taken from the heart to measure the biochemical factors (urea, creatinine, albumin, total protein). The results were analyzed using SPSS software and comparison of meanings was done using T. test. The results showed that the amount of albumin in the experimental group receiving 600 mg / kg of the extract decreased significantly compared to the control group. Total protein, cratinin and total body weight had no significant changes with control group. Hydro-alcoholic extracts of Passionate flower leaf caused reduction of blood urea nitrogen levels in all experimental groups receiving the extracts as compared to the control. According to the results of this study, it can be argued that the Passiflora caerulea plays a supporting role for the liver, and the lack of liver damage in the rats indicated this issue.

References:

. استرایر ال.، جرمی برگ جی.تی.، 1369. بیوشیمی. مترجمین: نوری م.، رهبانی م.، محبوب س.، جلد دوم، انتشارات احرار، ویرایش سوم، جلد دوم صفحات 765-390.

2. بزمی ف.، مختاری م.، خاتم­ساز س.، 1394. تاثیر عصاره آبی-الکلی گیاه گلپر در دوران بارداری بر میزان آنزیم­های کبدی و فاکتورهای بیوشیمیایی (آلبومین و پروتئین) در نوزادان موش­های صحرایی نر. مجله علوم پزشکی دانشگاه جهرم، جلد 13، شماره 1، صفحات 13-7.

3. رسولی ا.، 1370. بیوشیمی بالینی. انتشارات جعفری، چاپ اول، صفحات 765-390.

4. زاهدی م.ج.، حیدری م.ر.، مهاجری م.، 1382. تأثیر دو فراورده گیاهی سنبل­الطیب و گل­گاوزبان بر آزمون­های عملکرد کبد و کلیه در موش صحرایی. مجله دانشگاه علوم پزشکی کرمان، دوره یازدهم، شماره 1، صفحات 27-22.

5. صادقی ط.، 1390. تاًثیر تجویز خوراکی عصاره گل ساعتی بر تست­هـای عملکردی کبـد در مـوش  صحرایی نر بالغ (Rat). پایان­نامه کارشناسی­ارشد دانشگاه آزاد اسلامی واحد کازرون.

6. صادقی ط.، شریعتی م.، مختاری م.، 1395. تاثیر عصاره هیدروالکلی برگ گیاه گل ساعتی بر تست­های عملکردی کبد در موش صحرایی نر بالغ. نشریه زیست­شناسی جانوری، دوره 8 ، شماره  4، صفحات 78-71.

7. گایتون آ.، هال ج.، 1378. فیزیولوژی پزشکی، جلد دوم، انتشارات چهره، چاپ نهم، صفحه 1306.

8. Cauffield J.S.1., Forbes H.J., 1999. Dietary supplements used in the treatment of depression, anxiety, and sleep disorders. Lippincotts Prim Care Pract, 3(3): 290-304.

9. Dhawan K., Kumar S., Sharma A., 2004.  Passiflora: a review update. Journal of Ethnopharmacolog, 94(1): 1-23.

10. Garg A., Garg Szaneveld J., Singla A.K., 2001. Chemistry and Pharmacology of the citrus bioflavonoid hesperidin. Phytotherapy Research, 15(8):655-69.

11. Gross J.L., Azevedo M.J., Silviro S.B., Canai L.H., Caramori M.L., zelmanovitz T., 2005. Diabtic nephropathy: diagnosis, prevention, and treatment. Diabetes Care, 28(1): 164-76.

12. Grundmann O., Waehling C., Staiger C., Butterweck V., 2009. Anxi-olytic effects of a passion flower (Passiflora incarnata L.) extract in the elevated plus maze in mice. Pharmazie, 64: 63-64.

13. Johnson W.J., Hagge W.W., Wagoner R.D., Dinapoli R.P., Rosevear J.W., 1972. Effects of urea loading in patients with far- advanced renal failure.
Mayo Clinic Proceedings, 47(1): 21-9.

14. Kaneko J.J., 1989. Clinical biochemistry of domestic animals. 4th ed. New York: Academic Press Inc., 72: 144-389.

15. Limdi J.K., Hyde G.M., 2003 JunEvaluation of abnormal liver function tests. P.ostgraduate Medical Journal, 79(932): 307-12.

16. Raj Narayana K., Spiral Reddy M., Chaluvadi M.R., Krishna D.R., 2001. Bioflavonoids classification, pharmacological biochemical effects and therapeutic potential. Indian Journal of Pharmacology, 33: 2-16

17. Soulimani R., Younos C., Jarmoni S., Bousta D., Misslin R., Mortier F., 1997. Behavioural effects of passiflora incarnata L. and its indole alkaloid and flavonoid derivatives and maltol in the mouse. Journal of Ethnopharmacology, 57(1): 11-20.

18. Tzankaki E., 2000. Liver assist devices. Annual Review of Biomedical Engineering, 2: 607-632.

19. Tognohni M., Barocelli E., Ballabeni V., Bruni R., 2006. Comparative Screening of plant essential oils: Phenylpropanoid moiety as basis core for antiplatelet activity.
Life Sciences, 78(13):1419-32.

20. Uyanoglu M1, Canbek M, Aral E, Husnu Can Baser K. 2008 MarEffects of carvacrol upon the liver of rats undergoing partial hepatectomy. Phytomedicine, 15(3): 226-9.

21. Yam M.F., Basir R., Asmaw M.Z., Ismail Z., 2007. Antioxidant and hepato protective effects of orthosiphon stamineus Benth. Standardized extract. The American Journal of Chinese Medicine, 35(1): 115-26.