Effect of Zinc Supplementation on Hepatic Enzymes in Healthy Rats

Subject Areas : Journal of Animal Biology

H. Dabirinejad ¹ , M.R. Dayer ² , T. Mohammadi ³

1 - Department of Biology, Faculty of Science, Shahid Chamran University, Ahvaz, Iran
2 - Department of Biology, Faculty of Science, Shahid Chamran University, Ahvaz, Iran
3 - Department of Biology, Faculty of Science, Shahid Chamran University, Ahvaz, Iran

Received: 2018-04-21 Accepted : 2018-04-21 Published : 2018-02-20

Keywords: liver, Zinc deficiency, zinc sulfate, Rats,

Abstract :

Zinc ion is the second essential element in living organisms and is necessary for lots of enzymes activity e.g. alkaline phosphatase and lactate dehydrogenase. This ion also takes part in biological processes as human growth, immune response, and nerve function. Zinc deficiency causes significant decrease in serum alkaline phosphatase or increase in aspartate aminotransferase and alanine aminotransferase activity. Increase in these two later enzymes, in turn, is an indicator of tissue damage even though there are controversies among researchers. Accordingly, we decided to study the effect of 0.6 gm/L of zinc sulfate in drinking water in a period of 3 months treatment on serum activities of hepatic enzymes in adult rats. Twenty female rats were selected in two equal groups as control and treated groups. For a period of three months, zinc sulfate dissolved in tap water was used to drink by the treated group while the control group drank tap water. Both groups had full access to pellet food during this study. Serum activities of aspartate aminotransferase, alanine aminotransferase, alkaline phosphates and lactate dehydrogenase were measured finally. Zinc sulfate significantly decreased alanine aminotransferase while it showed no effects on the three other enzymes. Dietary zinc sulfate not only exerts no damage to liver but also as food supplement seems to have protective effect on this organ.  

References:

1. Asri-Rezaei S., Tamaddonfard E., Ghasemsoltani-Momtaz B., Erfanparast A. , Gholamalipour S., 2015. Effects of crocin and zinc chloride on blood levels of zinc and metabolic and oxidative parameters in streptozotocin-induced diabetic rats. Phytomedicine, 5(5):403

2. Cai L., Li X.K., Song Y., Cherian M.G., 2005. Essentiality, toxicology and chelation therapy of zinc and copper. Current medicinal chemistry, 12(23):.2753-2763

 

3. Chevalier C.A., Liepa G., Murphy M.D., Suneson J., VanBeber A.D., Gorman M.A,. Cochran C., 2002. The effects of zinc supplementation on serum zinc and cholesterol concentrations in hemodialysis patients. Renal Nutrition, 12(3):183-189

4. Cho Y.E., Lomeda R.A.R., Ryu S.H., Sohn H.Y., Shin H.I., Beattie J.H. ,Kwun, I.S., 2007. Zinc deficiency negatively affects alkaline phosphatase and the concentration of Ca, Mg and P in rats. Nutrition research and practice, 1(2):.113-119

5. Dean R.L., 2002. Kinetic studies with alkaline phosphatase in the presence and absence of inhibitors and divalent cations. Biochemistry and Molecular Biology Education, 30(6): 401-407

6. Derouiche S., Kechrid Z., 2016. Zinc Supplementation Overcomes Effects of Copper on Zinc Status, Carbohydrate Metabolism and Some Enzyme Activities in Diabetic and Nondiabetic Rats. Canadian journal of diabetes, 40(4):.342-347

7. Devi T., Hijam D., Dubey A., Debnath S., Oinam P., Devi N.G, Singh G., 2016. Study of Serum Zinc and Copper Levels in Type 2 Diabetes Mellitus. International Journal of Contemporary Medical Research, 3:1036-1040

8. El Hendy H.A., Yousef M.I, El-Naga N.I.A., 2001. Effect of dietary zinc deficiency on hematological and biochemical parameters and concentrations of zinc, copper, and iron in growing rats. Toxicology, 167(2): 163-170

9. Goldhaber S.B., 2003. Trace element risk assessment: essentiality vs. toxicity. Regulatory toxicology and pharmacology, 38(2):.232-242

 10. Jahromi ES., Jahromi SZ., Jahromi HK.,2014. Histopathological investigation of the effect of alkaline phosphatase on adult male rats' liver tissue based on enzyme inhibition. Journal of Jahrom University of Medical Sciences, 12(3):59-65

11. Li S.S.L., Hou E.W., 1989. Estrogen-induced expression of mouse lactate dehydrogenase-A gene. Cell biology international reports, 13(7): 619-624

12. Malhotra A. , Dhawan D.K., 2008. Zinc improves antioxidative enzymes in red blood cells and hematology in lithium-treated rats. Nutrition research, 28(1): 43-50

13. Maret W., 2000. The function of zinc metallothionein: a link between cellular zinc and redox state. The Journal of nutrition, 130(5): 1455S-1458S

14. Mocchegiani E., Muzzioli M. , Giacconi R., 2000. Zinc and immunoresistance to infection in aging: new biological tools. Trends in Pharmacological Sciences, 21(6): 205-208

15. Mokhtari M., Shariati M., Goshmandi N., 2005. Effects of zinc on the concentration of thyroid hormones and liver enzymes in male rats. Zanjan jornal of research in medical sciences, 13(51):7-12

16. Plum L.M., Rink L., Haase H., 2010. The essential toxin: impact of zinc on human health. International journal of environmental research and public health, 7(4): 1342-1365

17. Roozbeh J, Sharifian M., Karimi M., Hamidian Jahromi AR., Afshariani R.,2009. Effect of zinc supplementation on red blood cell osmotic fragility in hemodialysis patients. Shiraz Journal of  Medical Sciences, 10(4):186-189

18. Sirat sabet M., shir ouzhan sllakhouri PS. ,2007. Effects of electromagnetic field with 25,50 and100 hz frequency on serum alanine transaminase and Aspartate transaminase activity in mice. Zahedan jornal of research in medical sciences, 9(3):163-170

 

19. Tanada S., Higuchi T., Nakamura T., Imaki M., Matsumoto K., Miyoshi T., 1993. Evaluation of exercise intensity indicated by serum lactate dehydrogenase activity in healthy adults. Acta Biologica Hungarica, 44(2-3): 153-160