Evaluation of the Protective Effects of the Hydroalcoholic Extract of Securigera Securidaca L. Seeds on Alterations of Serum Biochemical Parameters and Renal Indices in Tamsulosin-Treated Male Rats

Subject Areas : Journal of Animal Biology

Sadigheh Khezri Motlagh ¹ , Mokhtar Mokhtari ² , Mehrdad Shariati ³

1 - Department of Biology, Kazerun Branch, Islamic Azad University, Kazerun, Iran
2 - Department of Biology, Kazerun Branch, Islamic Azad University, Kazerun, Iran
3 - Department of Biology, Kazerun Branch, Islamic Azad University, Kazerun, Iran

Received: 2022-07-24 Accepted : 2022-12-26 Published : 2023-05-22

Keywords: Rat, kidney, Lipid, tamsulosin, Securigera securidaca L,

Abstract :

Securigera securidaca L. seeds are rich in polyphenols and flavonoids that have many biological effects. The aim of this study was to evaluate the protective effects of hydroalcoholic extract of Securigera securidaca seed on alterations in serum biochemical parameters including glucose, lipid profile (cholesterol, triglyceride, LDL and HDL), renal function indices (creatinine and BUN) and antioxidant enzymes activity (Catalase, superoxide dismutase, and glutathione peroxidase) in tamsulosin-treated male rats. Seventy-two adult male rats were grouped into 9 groups of 8. The control group did not receive drug treatment but the sham group received 1 ml of extract solvent, the positive control group received 0.4 mg/kg Tamsulosin, negative control groups 1, 2, and 3 received 200, 400, and 800 mg/kg extract, respectively and experimental groups 1, 2 and 3 first received 0.4 mg/kg Tamsulosin and then 200, 400 and 800 mg/kg of extract for 28 days, respectively. Finally, blood samples were collected to measure the serum biochemical parameters. Tamsulosin increased the levels of glucose, creatinine and BUN, and decreased the level of antioxidant enzymes activity in the control group (p < 0.05), but did not cause significant changes in the level of lipid profile (p > 0.05). However, treatment with doses of 400 and 800 mg/kg of extract in experimental groups 2 and 3, improved glucose, creatinine, BUN and antioxidant enzymes activity compared to the control group (p < 0.05). Hydroalcoholic extract of Securigera securidaca seeds can improve changes in biochemical parameters and renal function indices in the serum of tamsulosin-treated male rats.

References:

1. Ahmadi A. Khalili M. Margedari S.H. Nahri-Niknafs B. 2016. The effects of solvent polarity on hypoglycemic and hypolipidemic activities of Securigera securidaca (L.) seeds. Drug Research, 66(03):130-135.


2.  


3. Alizadeh-Fanalou S., Babaei M., Hosseini A., Azadi N., Nazarizadeh A., Shojaii A., Bahreini E. 2020. Effects of Securigera Securidaca seed extract in combination with glibenclamide on antioxidant capacity, fibroblast growth factor 21 and insulin resistance in hyperglycemic rats. Journal of ethnopharmacology, 24(8):112-121.‏

 

3. Al-Jassabi S., Al-Omari A., Saad A., Azirun M.S. 2011. Tamsulosin-Induced hepatotoxicity and nephrotoxicity and its prevention by potato peel extract. Am-Euro J Toxicol Sci, 3(2):52-58.‏.

 

4. Babaei M., Alizadeh-Fanalou S., Nourian A., Yarahmadi S., Farahmandian N., Nabi-Afjadi M., Bahreini E. 2021. Evaluation of testicular glycogen storage, FGF21 and LDH expression and physiological parameters of sperm in hyperglycemic rats treated with hydroalcoholic extract of Securigera Securidaca seeds, and Glibenclamide. Reproductive Biology and Endocrinology, 19(1):1-15.‏

 

5. Bhat M., Zinjarde S.S., Bhargava S., Y., Kumar A.R., Joshi B.N. 2011. Antidiabetic Indian plants: a good source of potent amylase inhibitors. Evidence-Based Complementary and Alternative Medicine, 20(5):44-59.

 

6. Boschmann M., Krupp G., Luft F.C., Klaus S., Jordan J. 2002. In Vivo Response to α1‐Adrenoreceptor Stimulation in Human White Adipose Tissue. Obesity research, 10(6):555-558.‏

 

7. Bryan N.S., Grisham M.B. 2007. Methods to detect nitric oxide and its metabolites in biological samples. Free Radical Biology and Medicine, 43(5):645-657.‏

 

8. Cai Y., Luo Q., Sun M., Corke H. 2004. Antioxidant activity and phenolic compounds of 112 traditional Chinese medicinal plants associated with anticancer. Life sciences, 74(17):2157-2184.‏

 

9. Carlström M. 2021. Nitric oxide signalling in kidney regulation and cardiometabolic health. Nature Reviews Nephrology, 17(9):575-590.‏

 

10. Cheng J.T., Liu I.M., Yen S.T., Chen P. C. 2000. Role of α1A-adrenoceptor in the regulation of glucose uptake into white adipocyte of rats in vitro. Autonomic Neuroscience, 84(3):140-146.‏

 

11. Cice T., Gokturk H.S., Unler G.K. 2015. Acute hepatocellular drug induced liver injury probably by alfuzosin. Case Reports in Urology, 20(15):89-96.‏

 

12. Dikko M., Sarkingobir Y., Umar A.I.. 2020. Effect of Tamsulosin Administration on Oral Glucose Tolerance (OGT) In Normal Wistar Rats. Nigerian Journal of Physiological Sciences, 35(2):187-190.‏

 

13. Duan L., Zhang C., Zha Y., Chang Y., Guo L. 2020. Comparison of bioactive phenolic compounds and antioxidant activities of different parts of Taraxacum mongolicum. Molecules, 25(14):32-60.‏

 

14. El-Borady O.M., Othman M.S., Atallah H.H., Moneim A.E.A. 2020. Hypoglycemic potential of selenium nanoparticles capped with polyvinyl-pyrrolidone in streptozotocin-induced experimental diabetes in rats. Heliyon, 6(5):40-45.‏

 

15. Enroth S., Maturi V., Berggrund M., Enroth, S.B., Moustakas A., Johansson Å., Gyllensten U. 2018. Systemic and specific effects of antihypertensive and lipid-lowering medication on plasma protein biomarkers for cardiovascular diseases. Scientific Reports, 8(1):1-10.‏

 

16. Faintrenie G., Géloën A. 1998. Alpha-1 adrenergic stimulation of glucose uptake in rat white adipocytes. Journal of Pharmacology and Experimental Therapeutics, 286(2):607-610.‏

 

17. Fallah Huseini H., Hooseini P., Heshmat R., Yazdani D., Rahmani M. ,Hemati Moqadam H.R., Alavi S.H.R. 2006. The clinical investigation of securigera securidaca (L.)(degen & doerfler) seeds in type II diabetic patients a randomized, double-blind, placebo-controlled study. Journal of Medicinal Plants, 5(20): 75-79.‏

 

18. Farese R.V. 2001. Insulin-sensitive phospholipid signaling systems and glucose transport. Update II. Experimental Biology and Medicine, 226(4):283-295.‏

 

19. FlechtnerMors M., Jenkinson C.P., Alt A., Biesalski H.K., Adler G., Ditschuneit H. H. 2004. Sympathetic Regulation of Glucose Uptake by the α1‐Adrenoceptor in Human Obesity. Obesity Research, 12(4):612-620.‏

 

20. Franco-Salinas G. 2010. de la Rosette JJMCH & Michel MC Pharmacokinetics and pharmacodynamics of tamsulosin in its modified-release and oral controlled absorption system formulations. Clincal Pharmacokinetic, 49:177-188.‏

 

21. Garjani A., Fathiazad F., Zakheri A., Akbari N.A., Azarmie Y., Fakhrjoo A., Maleki-Dizaji N. 2009. The effect of total extract of Securigera securidaca L. seeds on serum lipid profiles, antioxidant status, and vascular function in hypercholesterolemic rats. Journal of ethnopharmacology, 126(3): 525-532.‏

 

22. Ghorbani A., Marjaneh R.M., Rajaei Z. 2014. Effects of securigera securidaca extract on lipolysis and adipogenesis in diabetic rats. Cholesterol, 20(14):145-162.‏

 

23. Hong Y.A., Park C.W. 2021. Catalytic Antioxidants in the Kidney. Antioxidants. 10:130-144.‏

 

24. Hosseinzadeh H., Ramezani M., Danaei A.R. 2002. Antihyperglycaemic effect and acute toxicity of Securigera Securidaca L. seed extracts in mice. Phytotherapy Research: An International Journal Devoted to Pharmacological and Toxicological Evaluation of Natural Product Derivatives, 16(8):745-747.‏

 

25. Hutchinson D.S., Bengtsson T. 2005. α1A-adrenoceptors activate glucose uptake in L6 muscle cells through a phospholipase C-, phosphatidylinositol-3 kinase-, and atypical protein kinase C-dependent pathway. Endocrinology, 146(2):901-912.‏

 

26. Kabra N.K. 2014. Alpha blockers and metabolic syndrome. J Associ Physi India, 62:13-16.


27. .


28. Katzung B.G. 2004. Drug receptors and pharmacodynamics. Basic and clinical pharmacology, 9nd ed. (McGraw-Hill Medical), p.17-19.

 

29. Kohestani Y., Kohestani B., Shirmohamadi Z., Faghani M. 2020. Effect of tamsulosin on testis histopathology and serum hormones in adult rats: Experimental study. International Journal of Reproductive BioMedicine, 18(7):531.‏

 

30. Lepor , Kazzazi A., Djavan B., 2012. α-Blockers for benign prostatic hyperplasia: the new era. Current opinion in urology, 22(1):7-15.‏

 

31. López A. Rico M. Santana-Casiano J. M. González A. G. González-Dávila M. 2015. Phenolic profile of Dunaliella tertiolecta growing under high levels of copper and iron. Environmental Science and Pollution Research, 22(19):14820-14828.‏

 

32. Michel M. C. de la Rosette J. J. 2004. Efficacy and safety of tamsulosin in the treatment of urological diseases. Expert Opinion on Pharmacotherapy, 5(1):151-160.‏

 

33. Moharir G. Bharatha A. Naikawadi A.A. Wali R.S. 2015. Effect of tamsulosin a selective α1-antagonist on glucose homeostasis in rats. Int J Pharm Pharm Sci; 7(3):232-234.

 

34. Nasehi Z. Kheiripour N. Taheri M. A. Ardjmand A. Jozi F. Aghadavod E. Shahaboddin M. E. 2022. The Protective Effects of Securigera securidaca Seed Extract on Liver Injury Induced by Bile Duct Ligation in Rats. BioMed Research International, 20(22):42-61.‏

 

35. Olayinka E. T. Adewole K. E. 2021. Ameliorative effect of morin on dutasteride-tamsulosin-induced testicular oxidative stress in rat. Journal of Complementary and Integrative Medicine, 18(2): 327-337.‏

 

36. Mahdi P. Mohammad E. S. Ali-Akbar M. Karim P. 2011. To study the effects of Securigera securidaca (L.) seed against alloxan-induced hyperglycemia. Journal of Medicinal Plants Research, 5(14): 3188-3191.‏

 

37. PubChem (Internet). Bethesda (MD): National Library of Medicine (US), National Center for Biotechnology Information; 2004-. PubChem Compound Summary for CID 129211, Tamsulosin. Available from: https://pubchem. ncbi.nlm. nih.gov/compound/129211.

 

38. Rasouli H., Hosseini-Ghazvini S. M.B., Adibi H., Khodarahmi R. 2017. Differential α-amylase/α-glucosidase inhibitory activities of plant-derived phenolic compounds: a virtual screening perspective for the treatment of obesity and diabetes. Food & function,8(5): 1942-1954.‏

 

39. Roostazadeh A., Firoozrai M., Shabani M. 2008. Effect of aqueous seed extract of Securigera Securidaca on erythrocytes catalase activity in type 1 diabetic rats. Qom University of Medical Sciences Journal, 1(4):9-14.‏

 

40. Rosen R.C., Wei J.T., Althof S.E., Seftel A.D., Miner M., Perelman M.A., Patient Survey Steering Committee. (2009). Association of sexual dysfunction with lower urinary tract symptoms of BPH and BPH medical therapies: results from the BPH Registry. Urology, 73(3):562-566.‏

 

41. So M.J., Cho E.J. 2014. Phloroglucinol attenuates free radical-induced oxidative stress. Preventive nutrition and food science, 19(3):129-143.‏

 

42. Sun L., Sun C., Zhou S., Zhang L., Hu W. 2021. Tamsulosin attenuates high glucose-induced injury in glomerular endothelial cells. Bioengineered, 12(1): 5184-5194.‏

 

43. Tofighi Z., Moradi-Afrapoli F., Ebrahimi S.N., Goodarzi S., Hadjiakhoondi A., Neuburger M., Yassa N. 2017. Securigenin glycosides as hypoglycemic principles of Securigera securidaca seeds. Journal of natural medicines, 71(1): 272-280.‏

 

44. Tungmunnithum D., Thongboonyou A., Pholboon A., Yangsabai A. 2018. Flavonoids and other phenolic compounds from medicinal plants for pharmaceutical and medical aspects: An overview. Medicines, 5(3):93-107.‏

 

45. Zahedi-Asl S., Marahel H., Zaree B. 2005. Study on the effects of chloroformic extract of Securigera Securidaca on serum glucose level and liver glycogen content of mice. Journal of Kerman University of medical sciences, 11(1):32-38.‏


46. Zhong H., Minneman K.P. 1999. Alpha1-adrenoceptor subtypes. Eur J Pharmacol; 375(1-3):261-76.