Comparative Study of Pituitary Extract, LHRH, and Thyroxine on Sex steroids, Histochemistry and (Labeo rohita) Producing Fatty Acids

Subject Areas : Journal of Animal Biology

Ehsan  Eslamizadeh ¹ (Department of Fisheries, Ahvaz Branch, Islamic Azad University, Ahvaz, Iran)
Hadideh  Mabudi ² (Department of Fisheries, Ahvaz Branch, Islamic Azad University, Ahvaz, Iran)
Laleh  Roomiani ³ (Department of Fisheries, Ahvaz Branch, Islamic Azad University, Ahvaz, Iran)
Mehran  Javaheri Baboli ⁴ (Department of Fisheries, Ahvaz Branch, Islamic Azad University, Ahvaz, Iran)
Mojdeh  Cheleh Mal Dezfooli Nezhad ⁵ (Department of Fisheries, Ahvaz Branch, Islamic Azad University, Ahvaz, Iran)

Received: 2021-07-05 Accepted : 2021-08-06 Published : 2022-08-23

Keywords: Hormone, Thyroxine, Fatty Acids, Labeo rohita, LHRH, Pituitary,

Abstract :

This study aimed at assessing the comparative effect of pituitary hormones, LHRH, and thyroxine on hormonal indices and ovarian fatty acids of Labeo rohita. Blood samples were taken before injection, between the first and second injection, after the second injection and after ovulation, using a 5 ml plastic syringe from the tail stem. According to the measurement results, at the time between the first and second injections, after the second injection and after ovulation, the levels of estradiol, testosterone, 17-alpha hydroxy progesterone, cortisol and LH reached their peak levels in the pituitary and treatment receiving 50 μg dose had the lowest body weight in treatments receiving LHRH and control (P <0.05). Among thyroxine-receiving treatments, increasing thyroxine levels could significantly increase the hormones stimulating the maturation process (P <0.05). Pituitary receiving treatment and thyroxine receiving 50 μg per body weight had the highest amount of ovarian protein (P <0.05), with 16.70 and 15.99% dry weight and two LHRH and control treatments had the lowest ovarian protein level (P <0.05). Regarding fat, thyroxine-receiving treatments had a significantly higher amount compared to pituitary, LHRH, and control (P <0.05). The highest amount of saturated fatty acids (ΣSUF) with values ​​of 63.15 and 58.73% of dry weight to the pituitary receiving treatment and receiving 50 μg per body weight and the lowest amount of this parameter with 54.55 and 50.14% dry weight belonged to the control and LHRH recipient, respectively. C17: 1 and C18: 1n9c fatty acids in the range of 3.43-4.77 and 3.28-5.40% of dry weight and C24: 1n9 fatty acids in the range of 0.03-0.18% by dry weight had the highest and lowest levels of unsaturated fatty acids (ΣPUF), respectively. Both pituitary and 50 μg treatments of thyroxine had higher total omega-6 (Σω6) and 3 (Σω3) fatty acids (P <0.05). The results of the present study revealed that pituitary hormone followed by thyroxine at a dose of 50 μg per body weight compared to low doses of thyroxine and LHRH were more effective in increasing the level of sex hormones involved in the process of sexual maturation and the combination of acids. 

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