The Effect of Movento Insecticide on the Fertility Rate in Male Balb/c Mice

Subject Areas : Journal of Animal Biology

Ghodrat Ebadi Manas ¹ (Department of Biology, Farhangian University, 14556-889 P.Box, Tehran, Iran)

Received: 2023-07-13 Accepted : 2023-08-23 Published : 2024-02-20

Keywords: Embryo, Fertility, insecticide, Movento, Male mouse,

Abstract :

Today, due to the indiscriminate use of poisons and the resistance of pests to them, new types of poisons are produced every year. Movento is the latest insecticide that is used in pistachio orchards to control the psyllid pest, whose side effects on the reproductive system have not been investigated. The aim of this study was to investigate the effect of Movento on fertility in Balb/c mice. In this study, 18 adult and healthy male Balb/c mice were prepared and kept under standard conditions. Each group consisted of 9 mice. Mice were randomly divided into experimental and control groups. The experimental group received 79 mg/kg Movento and the control group received the same amount of distilled water daily for 60 days through oral gavage. At the end of the study, the mice were euthanized by injecting a mixture of xylazine and ketamine. Epididymal tail of the testes of mice was separated and placed in HTF culture medium. The parameters of sperm count, sperm viability, DNA damage of sperm, immature sperms and fertilization ability of sperms and blastocyst were evaluated. The data obtained from the study were analyzed by ANOVA method. The findings showed that sperm viability, the percentage of fertilized oocytes, two-celled and four-celled embryos, and the number of blastocysts were significantly reduced in the experimental group compared to the control. Also, sperms count in the experimental group compared to the control group decreased but was not significant. However, immature sperms and DNA damage of sperm in the experimental group was increased compared to the control group. Therefore, it can be concluded that Movento poison reduces fertility in mice by DNA damage of sperm and chromatin.

References:

1. Beers E.H., Schmidt R.A. 2014. Impacts of orchard pesticides on Galendromus occidentalis: Lethal and sublethal effects. Crop protection, 56:16-24.


2. Bekhakheche , Manseur A., Masna F., Habbachi S., Habbachi W., Bairi A., ahraoui A. 2018. Chronic Contamination in Rats by Reduced Risk Pesticides: Cases of Spirotetramat and Citrullus Colocynthis (Cucurbitaceae) Extracts. World Journal of Environmental Biosciences, 6(4):1-6.


3. Bell J.W. 2013. Petition for a three-year extension of exclusive use data protection for spirotetramat as provided for under fifra section 3(c)(1) (F) (ii). Bayer Corp Science, 48 p.


4. Bretschneider T., Fischer R., Nauen R. 2007. Inhibitors of lipid synthesis (acetyl-CoA-carboxylase inhibitors). Modern Crop Protection Compounds, 3:909-926.


5. Falcón-Etchechury M., Silveira-Gramont M., Robles-Sánchez , Canett-Romero R., Ramos-Enríquez  R., López-Cervantes  G., Aldana-Madrid M.L. 2013 . Spirotetramat induce cambios histológicos y bioquímicos en rata Wistar. Revista de Toxicología, 30(2):215-217.


6. Fenner K., Canonica S., Wackett L.P., Elsner M. 2013. Evaluating pesticide degradation in the environment: blind spots and emerging opportunities.  Science, 341(6147):752-758.


7. González-Marín B., Calderón-Segura M. E., Pérez A.K.G., Ciénega L.G.M. 2021. Movento® 240SC (Spirotetramat) and Envidor® 240SC (Spirodiclofen) keto-enol insecticides induce DNA damage in Drosophila melanogaster ovaries. Fundamental Toxicological Sciences, 8(3): 81-88.


8. Goroohi F., Imani S., Samih M.A., Panahi B. 2018. Effects of ethanolic extracts of Rubia tinctorum, Ferula gummosa and Nesidiocoris tenuis (Hem.: Miridae) on sweet potato whitefly, Bemisia tabaci (Hem.: Aleyrodidae), and comparison with Spirotetrmat. IAU Entomological Research Journal, 10(2):111-125.


9. Hodges , Bell H., Adam K. 2012. Petition for a Three-Year Extension of Exclusive Use Data Protection for Spirotetramat. US Environmental Protection Agency Office of Pesticide Programs, Petition for Spirotetramat. 23 pp.


10. Jiang M., Chen S., Li J., Liu L. 2020. The biological and chemical diversity of tetramic acid compounds from marine-derived microorganisms. Marine Drugs, 18(2):114.


11. Kaboli Kafshgiri S., Parivar K., Baharara J., Hayati Roodbari N., Kerachian M.A. 2017. Comparison the effect of movento, a chemical pesticide, with chitosan, a biologic pesticide, on female reproductive system in Balb/C mice. Nova Biologica Reperta, 3(4):279-287.


12. Manas E., Hasanzadeh S., Parivar K. 2013. The effects of pyridaben pesticide on the histomorphometric, hormonal alternations and reproductive functions of BALB/c mice. Iranian Journal of Basic Medical Sciences, 16(10):1055.


13. Mannucci A., Argento FR., Fini E., Coccia ME., Taddei N., Becatti M., Fiorillo C. 2022. The impact of oxidative stress in male infertility. Frontiers in Molecular Biosciences, 8:799294.


14. Mohapatra , Deepa M., Jagadish G. K. 2012. An efficient analytical method for analysis of spirotetramat and its metabolite spirotetramat-enol by HPLC. Bulletin of Environmental Contamination and Toxicology, 88:124-128.


15. Moustafa M.H., Sharma R.K., Thornton J., Mascha E., Abdel-Hafez M.A., Thomas A.J. and Agarwal A. 2004. Relationship between ROS roduction, apoptosis and DNA denaturation in spermatozoa from patients examined for infertility. Human Reproduction, 19(1): 129-138.


16. Nauen R., Jeschke P., Velten R., Beck M.E., Ebbinghaus‐Kintscher U., Thielert W., Raupach G. 2015. Flupyradifurone: a brief profile of a new butenolide insecticide. Pest Management Science, 71(6): 850-862.


17. Nazari P., Poorjavad N., Izadi H., Sahafi S.R. 2020.Comparison of the effect of several common insecticides on Lepidosaphes malicola (Hem: Diaspididae) in Semirom apple orchards, Plant Pest Research, 1(10):17-30. (In Persian).


18. Ribas-Maynou J., Garcia-Bonavila E., Hidalgo CO., Catalán J., Miró J., Yeste M. 2021. Species-specific differences in sperm chromatin decondensation between eutherian mammals underlie distinct lysis requirements. Frontiers in Cell and Developmental Biology, 9:669182.


19. Sverdrup E., Bjorge  C., Eklo  O.M., Grung  M., Kallqvist T., Klingen I., Lag M. and Ropstad  E. 2012. Risk assessment of the insecticide Movento 100 SC with the active substance spirotetramat. – Bayer Crop Science Journal, 13:1-25.


20. Toshimori K. 2009. Dynamics of the Mammalian Sperm Head. Advances in Anatomy, Embryology and Cell Biology. Springer, Berlin, Heidelberg, 204:9-50.


21. Willmott A.L. 2012. Efficacy of systemic insecticides against the citrus mealybug, Planococcus citri, and pesticide mixtures against the western flower thrips, Frankliniella occidentalis. Ph.D dissertation, Faculty of protected environments, Kansas State University.


22. Zhang J., Qian L., Wang C., Teng M., Duan M., Chen X., Li X. and Wang C. 2020. UPLC-TOF-MS/MS metabolomics analysis of zebrafish metabolism by spirotetramat. Environ. Pollution, 2:266.