Evaluation of Antibody Titer from Newcastle Vaccine and Histological Effect in Sebright Chickens
Subject Areas : Journal of Animal BiologyKimia Karimi Sani 1 , Mehdi Rezaei 2 , Mohammadreza Hossenchi 3
1 - Department of Veterinary Medicine, Urmia Branch, Islamic Azad University, Urmia, Iran
2 - Department of Veterinary Medicine, Urmia Branch, Islamic Azad University, Urmia, Iran
3 - Department of Veterinary Medicine, Urmia Branch, Islamic Azad University, Urmia, Iran
Keywords: antibody titer, Histopathology, Newcastle, sebright chicken,
Abstract :
Newcastle disease (ND) is one of the most important viral disease which make lots of casualty in poultry flocks every year. Bird vaccination is one of the main way to prevent the damages. The aim of this research is to evaluation of antibody titer produced by Newcastle vaccine and histopathological effects in Sebright chickens. In this research, 72 one-day-old Sebright chickens were tested in a completely random format in two groups (with three repetitions). From day one to the end of period, the breeding conditions were the same for all Saberite chickens and the differences between the groups were only in the Newcastle disease vaccination program. Vaccination in the first group was the use of clone vaccine in the form of eye drops in one day, the injection of inactivated Newcastle vaccine together with the clone vaccine in the form of eye drops in 8 days, and the use of one vaccine in the form of drinking in 15 days. The second group was considered as the control group (without vaccination). The agglutination inhibition (HI) test was performed on the serum samples obtained after two blood draws on days 25 and 35, following vaccination. Also, histopathological biopsy was performed from the intestines. The results of statistical analysis by Tukey test showed that the average titer of Newcastle antibody in the vaccinated groups was significantly different from the control group (p < 0.01). Also, the results of morphometry and morphology of Newcastle vaccine in the intestinal tissue in the vaccinated groups showed a significant difference compared to the control group (p < 0.05). This study presents as a monitoring and strategy in terms of antibody production titer and histological effects after vaccination in Sebright farms.
1. Alexander D.J. 2000. Newcastle disease and other avian paramyxoviruses. Revue Scientifique et Technique, 19(2):443-462.
2. Alexander D.J., Senne D.A. 2008. A Laboratory Manual for the Isolation, Identification and Characterization of Avian Pathogens. 5th ed. American Association of Avian Pathologists, pp:135-141.
3. Alexander D.J., Jones R.C. 2008. Poultry Diseases. 6th ed. Saunders ELSEVIER. pp: 294-305.
4. Butcher G.D., Mile R.D. 2018. The Avian Immune System. Florida, Ifas Extension University of Florida, 74:1-2.
5. Ekarius C. 2016. Poultry breeds: chickens, ducks, geese, turkeys: The pocket guide to 104 essential breeds. Storey Publishing, LLC.
6. Erf GF. 2004. Cell-mediated immunity in poultry. Poultry Science, 83(4):580-590.
7.Ghalyanchi Langeroudi A., Hossein H., Karimi V., Madadgar O., Hashemzadeh M., Ghafouri S.A., Bagheri S.S., Vahedi S.M. 2014. Phylogenetic study based on the phosphoprotein gene of Iranian Newcastle disease viruses (NDV) isolates, 2010 -2012', Iranian Journal of Veterinary Medicine, 8(2):73-77.
8. Hussein E.A., Hair-Bejo M., Adamu L.A.R., Omar A.R., Arshad S.S., Awad E.A., Aini I. 2018. Scoring System for LesionsInduced by Different Strains of Newcastle Disease Virus in Chicken. Veterinary Medicine International, (1):1-9.
9. Kapczynski D.R., Afonso C.L., Miller P.J. 2013. Immune responses of poultry to Newcastle disease virus. Developmental and Comparative Immunology, 41(3):447-453.
10. Khader M.A., El-Kady M.F., Shaheed I.B. 2021. Comparative study between the post vaccinal histopathological alterations in some broiler chickens’ tissues induced by lasota and vg/ga newcastle disease vaccinal strains. Advances in Animal and Veterinary Sciences, 9(8):1135-1142.
11. Leslie J. 2000. Newcastle disease: Outbreak losses and control policy costs. Veterinary Record, 146(2):603-606
12. Mohammadamin O.G., Qubih T.S. 2011. Histopathology of virulent Newcastle disease virus in immune broiler chickens treated with IMBO®. Iraqi Journal of Veterinary Sciences, 25(1):9-13.
13. Miller P.J., Afonso C.L., El Attrache J., Dorsey K.M., Courtney S.C., Guo Z., Kapczynski D.R. 2013. Effects of Newcastle Disease Virus Vaccine Antibodies on the Shedding and Transmission of Challenge Viruses, Developmental and Comparative Immunology, 41(4):505-513.
14. Perozo F., Marcano R., Afonso C.L., 2012. Biological and phylogenetic characterization of a genotype VII Newcastle disease virus from Venezuela: efficacy of field vaccination. Journal of Clinical Microbiology, 50(4): 1204-1208.
15. Perozo F., Villegas P., Dolz R., Afonso C.L., Purvis L.B. 2008. The VG/GA strain of Newcastle disease virus: mucosal immunity, protection against lethal challenge and molecular analysis. Avian Pathology, 37(3):237-245.
16. Ratih D., Handharyani E., Setiyaningsih S. 2017. Pathology and immunohistochemistry study of Newcastle disease field case in chicken in Indonesia. Veterinary World, 10(9):1066-1071.
17. Roohani K., Tan S.W., Yeap S.K., Ideris A., Bejo M.H., Omar A.R. 2015. Characterisation of genotype VII Newcastle disease virus (NDV) isolated from NDV vaccinated chickens, and the efficacy of LaSota and recombinant genotype VII vaccines against challenge with velogenic NDV. Journal of veterinary Science, 16(4): 447-457.
18. Samadi S., Kianizadeh M., Fathi Najafi M., Mousavi Nasab S.D., Davatgar A.M.H., Royaee A., Pilvar P. 2014. Molecular characterization and phylogenetic study of velogenic Newcastle disease virus isolates in Iran. Virus Genes, 48(2):290-295.
19. Sarcheshmei M., Dadras H., Mosleh N., Mehrabanpour M.J. 2016. Comparative Evaluation of the Protective Efficacy of Different Vaccination Programs Against a Virulent Field Strain of the Newcastle Disease Virus in Broilers. Brazilian Journal of Poultry Science, 18(1):363-370.
20. Sharma J.M. 1991. Overview of the Avian Immune System. Veterinary Immunology and Immunopathology, 30(2):7-13.
21. Scott T.R. 2004. Our current understanding of humoral immunity of poultry. Poultry Science, 83:574-579.
22. Suarez D.L. 2020. Diseases of Poultry. 14th ed. WILLY Blackwell, pp: 112-166.
23. Thayer S.G., Bread C.W. 2008. A Laboratory Manual for the Isolation, Identification and Characterization of Avian Pathogens. The American Association of Avian Pathologists, 23(3): 221-232.
24. Winterfield R.W., Dhillon A.S. 1981. Comparative Immune Response from Vaccinating Chickens with lentogenic Newcastle Disease Virus Strains. Poultry Science, 60(2):1195-1203.
