Comparison of the Efficiency of Ruminal and Faecal Microbial Population from Sheep to Estimate the Nutritional Value of Corn and Sorghum Grains Using In Vitro Gas Production Technique

Subject Areas : Journal of Animal Biology

Abolfazl Aghajanzadeh-Golshani ¹ , Naser Maheri-Sis ² , Ramin Salamat Doust-Nobar ³ , Yahya Ebrahimnezhad ⁴ , Abolfazl Ghorbani ⁵

1 - Department of Animal Science, Shabestar Branch, Islamic Azad University, Shabestar, Iran
2 - Department of Animal Science, Shabestar Branch, Islamic Azad University, Shabestar, Iran
3 - Department of Animal Science, Shabestar Branch, Islamic Azad University, Shabestar, Iran
4 - Department of Animal Science, Shabestar Branch, Islamic Azad University, Shabestar, Iran
5 - Department of Animal Science, Shabestar Branch, Islamic Azad University, Shabestar, Iran

Received: 2021-04-14 Accepted : 2021-08-11 Published : 2022-02-20

Keywords: in vitro gas production, metabolizable energy, Fermentation, Rumen liquor, Faeces suspension,

Abstract :

This study aimed to investigate the fermentation efficiency of faecal microorganisms in comparison with rumen microorganisms to estimate the nutritional value of corn and sorghum grains in ruminants using in vitro gas production technique. To perform in vitro gas production technique with rumen liquor and faecal suspension, rumen fluid and fresh faeces were collected from three fistulated Gezel rams. The results of this study showed that there are no significant differences due to in vitro gas production between the two methods at different incubation times in experimental feedstuffs. There was no significant difference between two methods in terms of the amount of gas production from fermentable fraction (A) for corn grain, while the amount of this parameter by fecal suspension was significantly higher than that of rumen liquor in sorghum grain (P<0.05). Estimated metabolizable energy (ME), net energy for lactation (NEL), organic matter digestibility (OMD), and short chain fatty acids (SCFA) contents of experimental grains with rumen fluid and faecal suspension showed no significant differences.The amount of gas produced with rumen liquor can be successfully estimated from faeces suspension using the obtained equations Ysorghum=0.8929 X -1.6353 for the sorghum grain and Ycorn=0.9657X-3.4097 for corn grain. According to the results of the study, it seems that the animal faeces suspension has the potential to replace rumen liquor in the in vitro gas production technique for ruminants feed evaluation.

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