Effects of Eight Weeks of High-Intensity Interval Training (HIIT) on the Expression of GPR120 and AMPK Proteins in the Heart Tissue of Diabetic Male Rats

Subject Areas : Journal of Animal Biology

Ebrahim Hoseini Houri pasand ¹ , Saeid Dabbagh Nikoo Kheslat ² , Javad Vakili ³

1 - Department of Exercise Physiology, Faculty of Physical Education and Sport Sciences, University of Tabriz, Tabriz, Iran
2 - Department of Exercise Physiology, Faculty of Physical Education and Sport Sciences, University of Tabriz, Tabriz, Iran
3 - Department of Exercise Physiology, Faculty of Physical Education and Sport Sciences, University of Tabriz, Tabriz, Iran

Received: 2021-11-01 Accepted : 2022-11-02 Published : 2023-05-22

Keywords: Atherosclerosis, Type 2 diabetes, High Intensity Interval Training, Reverse Cholesterol Transfer,

Abstract :

The reverse cholesterol transfer process has a positive role in reducing atherosclerotic plaques during diabetes. Therefore, the aim of the present study was to evaluate the effect of eight weeks of high-intensity interval training on GPR120 and AMPK proteins in the cardiac tissue of mice with type 2 diabetes. In an experimental study, 40  three-month-old adult male Wistar rats with an average weight (250-300 g) were randomly divided into four groups of 10 series including: healthy control (C: intraperitoneal injection of saline), healthy training (T: running at 85-90 % of maximum speed in 6 to 12 bouts in two-minute periods; 5 days per week for eight weeks), diabetic control (D: diabetic on a high-fat diet with intraperitoneal injection of streptozotocin) and trained diabetics (D+T: diabetic with training) were divided. A method based on Western blotting was used to determine changes in the expression profile of GPR120 and AMPK proteins in the heart muscle tissue (left ventricle) of rats. The two-way analysis of variance and Bonferroni post hoc test were used to analyze the data. Induction of diabetes (D) significantly reduced GPR120 and AMPK proteins (p ≤ 0.05). While HIIT training apply in healthy group (T) increased 81% and 47% in GPR120 and AMPK compared to diabetic control group (D) respectively (p = 0.001). Also, training intervention has the ability to prevent a decrease in both of proteins in the trained diabetic group (D+T) compared to the diabetic group (D) (p = 0.023). Based on the findings of this study, it can be stated that eight weeks of high-intensity interval training (HIIT) is a preventive strategy against reducing the activity of GPR120 and AMPK proteins involved in the reverse cholesterol transmission during type 2 diabetes. 

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