The Effect of Eryngium Campestre Hydroalcoholic Extract on Seizure Due to by Pentylene Tetrazole Injection in Adult Male Rats

Subject Areas : Journal of Animal Biology

Sara Zandi ¹ , Saeid Valipour Chahardahcharic ² , Maryam Rafieirad ³

1 - Department of Biology, Izeh Branch, Islamic Azad University, Izeh, Iran
2 - Department of Biology, Izeh Branch, Islamic Azad University, Izeh, Iran
3 - Department of Biology, Izeh Branch, Islamic Azad University, Izeh, Iran

Received: 2020-10-16 Accepted : 2021-01-20 Published : 2021-11-22

Keywords: Mice, Seizure, Eryngium Campestre, Pentylene Tetrazole,

Abstract :

Epilepsy is the most common disorders of the nervous system. The side effects of some drugs and their resistance to them have been considered by plants for identifying drugs with minimal side effects. This study investigated the effect of hydroalcoholic extract of E. campestre on pentylenetetrazol-induced seizures in male mice. In the present experimental study, 40 male Wistar rats weighing 25-30 g were randomly divided into 5 groups (n = 8): saline, three epileptic groups treated with doses of 50, 100, and 200 mg/kg of E. campestre extract and positive control group treated with 1 mg/kg diazepam. In order to create a model of epilepsy, Pentylenetetrazol (PTZ) was injected with dose of 85 mg/kg. The effect of different doses of extract and drug on the severity of seizures was evaluated by measuring the duration of different seizure behavioral states in the animal. All prescriptions were given intraperitoneally (ip). The groups treated with different doses of E. campestre extract as well as the diazepam treated group showed a significant increase in delayed onset of seizure, tonic duration, clonic duration, and tonic-clonic duration, and length Seizure time and survival rate were significantly higher in the saline group. The results of this study showed that E. campestre extract increase delayed onset of seizure and reduced mortality due to seizure in mice.

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