The Effect of Gelatin-aloevera Nanofibers on Collagen I Gene Expression in the Skin of Male Mice after Microneedling

Subject Areas : Journal of Animal Biology

Asadolah Shahrani ¹ , Maryam Bananej ² , Parvin Mansouri ³ , Henghameh Alibeik ⁴ , Nahid Nickhah ⁵

1 - Department of Biology, North Tehran Branch, Islamic Azad University, Tehran, Iran
2 - Department of Biology, North Tehran Branch, Islamic Azad University, Tehran, Iran
3 - Skin and Stem Cell Research Center, Tehran University of Medical Sciences, Tehran, Iran
4 - Department of Biology, North Tehran Branch, Islamic Azad University, Tehran, Iran
5 - Skin and Stem Cell Research Center, Tehran University of Medical Sciences, Tehran, Iran

Received: 2020-09-27 Accepted : 2021-01-06 Published : 2021-08-23

Keywords: PCR, Skin, Nanofibers, Microneedling, Gelatin-alo vera, Collagen І,

Abstract :

The skin is prone to mechanical injuries and damage and the skin collagen changes with age. Herbs and aloe vera gel are used experimentally and traditionally in the skin repair and rejuvenation. In this study, the effect of gelatin-aloe vera nanofibers on the expression of collagen I gene after skin microneedling was investigated in male mice under standard laboratory conditions. Skin microneedling was performed on the backs of the tested mice. Groups were subjected to treatments with saline, aloe vera gel, and gelatin-aloe vera nanofibers for 48 h. The expression of collagen I gene was evaluated through sampling and purification of tissue RNA by Real time PCR method. According to the results, skin injury resulted in a significant reduction in the expression of the collagen I gene in the treatment groups compared to the control group. In comparison, treatment with aloe vera gel and aloe vera/gelatin nanofibers significantly increased gene expression compared to the model group. Furthermore, the existing Glucomannan increased gene expression at the site of injury by passing through pores.

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