Effects of Flaxseedon OogenesisandOvaryTissuein NMRI AdultMice In vivo and In vitro
Subject Areas : Journal of Animal Biologyمعظمه خوش روش 1 , نسیم حیاتی رودباری 2 , مرجان صباغیان 3 , کاظم پریور 4
1 - گروه زیست شناسی، واحد علوم و تحقیقات تهران، دانشگاه آزاد اسلامی، تهران، ایران
2 - گروه زیست‌شناسی، واحد علوم و تحقیقات تهران، دانشگاه آزاد اسلامی، تهران، ایران
3 - پژوهشگاه رویان، مرکز تحقیقات پزشکی تولیدمثل، گروه آندرولوژی، تهران، ایران
4 - گروه زیست شناسی، واحد علوم و تحقیقات تهران، دانشگاه آزاد اسلامی، تهران، ایران
Keywords: Fertility, Mice, Ovary, Flaxseed,
Abstract :
Use of medicinal plants has increased sharply in recent years. However, for the uses of flaxseed as a functional food, its side effects on human fertility must be checked.That's why we decided to study the effect of flaxseed on the reproductive system in female mice.Our experimentswere performed on two conditions in vivo and in vitroMice in 3 different groups have received mice food for 14 days. We used flaxseed, which there are 3.33, 16.66 and 33.3 mg/kg density of flaxseed in 0.5 ml distilled water. Micedescribedonday15.The histological evaluation of control, sham and experimental groups was performed.In vitroovaries were cultured in sterile conditions for 24 hours, and thenwere studied.A significant increase in the number and diameter of primordial follicles, number of primary follicles,, Demolished follicles, Yellow body, and showed decrease in Animal weight, number of Secondary follicles, number and length of Graafian follicles, primary follicles Length, diameter Graffian and yellow body length in vivo.The number of demolished follicles increase in in vitro.The results of this study indicate that flaxseed have effects on oogenesis and ovary tissue and shouldbe used during pregnancy with caution.
