Protective Effect of Tribulus terrestris Hydroalcoholic Extract on Cisplatin- induced Cytotoxicity on Tissue Testis in Mice

Subject Areas : Journal of Animal Biology

زهرا کشتمند ¹ , شهربانو عریان ² , علی قنبری ³ , مظفر خزاعی ⁴

1 - دانشگاه آزاد اسلامی، واحد علوم و تحقیقات تهران، گروه زیست شناسی، تهران، ایران
2 - دانشگاه خوارزمی، دانشکده علوم زیستی، گروه زیست شناسی، تهران، ایران
3 - مرکز تحقیقات باروری و ناباروری، دانشکده علوم پزشکی کرمانشاه، کرمانشاه، ایران
4 - مرکز تحقیقات باروری و ناباروری، دانشکده علوم پزشکی کرمانشاه، کرمانشاه، ایران

Received: 2014-08-20 Accepted : 2017-05-21 Published : 2014-07-23

Keywords: Mice, Testis, Cisplatin, Tribulus terrestris,

Abstract :

 Cisplatin is an anti-cancer drug used in chemotherapy. One of the limiting side effects of cisplatin use is cytotoxicity and decrease in genital gland function, azoospermia and oligospermia. Tribulus terrestris has been used as an aphrodisiac.the present study amid to investigateprotective effect of T. terrestris hydroalcoholic extract on cisplatin- induced cytotoxicity on tissue testis in mice. In this experimental  study,thirty male adult mice with weight 25-30 g were randomaly into five groups of six each Control group reicived saline , first  experimental group  received cisplatin (5.5 mg/kg) and other  three experimental group ,that received different doses of hydroalcoholic extact of T. terrestris 100-300 and 500 (mg/kg/i.p) with cisplatin  resepctively.Day after the last injection, weights of body and  testis and testise tissue histology  were assessed. Data analysis was performed using SPSS and one-way ANOVA followed. The results showed that cisplatin  leaded to a reduction  significantly in the weights of body (PT. terrestris, the weights of body and testis, seminiferous tubular diameter were increased compared with cisplatin group and  the number of Multinucleated cells in the seminiferous lumen  and epithelium vacuolization  show decreased  compared cisplatin group. The result showed thatextract of T. terrestris could effect of protective on cisplatin- induced cytotoxicity on tissue testis that may be related to the presence of antioxidant­ components.

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