Evaluation of colonization ability of lactobacilli isolated from traditional cheese and adherence to epithelial cells of human GI tract, Caco-2 cell line

Subject Areas : Journal of Animal Biology

میترا حیدری نصرآبادی ¹ , مریم تاج آبادی ابراهیمی ² , هدی بهرامی ³

1 - استادیار دانشکده زیست شناسی دانشگاه آزاد اسلامی واحد پرند
2 - استادیار دانشکده علوم دانشگاه آزاد اسلامی واحد تهران مرکزی
3 - کارشناس دانشکده علوم دانشگاه آزاد اسلامی واحد تهران مرکزی

Received: 2014-06-23 Accepted : 2017-05-21 Published : 2009-07-23

Keywords: adherence, Colonization, Lactobacillus, Caco-2 cell line,

Abstract :

The aim of this study was to assesandrsquo;colonization ability of 7 lactobacilli strain isolated from traditional cheese in GI[1] trace. So resistance to acidic pH, bile salt and ability to adhere Caco-2 cell line was assessed in vitro. 2 hour acidic treatments lead to 0-4 log reduction of tested strains. Isolate B1D2was fully resistance and no reduction in population observed after acid treatment. Bile salt cause growth delay between 25-180 min for all strains and BH1with D= 25 andplusmn; 4.80 min was the most resistance strain. Ability to adhere Caco-2 cell line was strain depended and GI2 and SL2 were less and most adherent strain respectively. In conclusion evaluation of these 3 factor in vitro indicated that SM3, BD2, SM1, SL2 isolated from Semnan and Babol cheese have colonization ability