Effect of Ascorbate and Gibberellin on antioxidant activity, proline and soluble carbohydrates in Sage (Salvia officinals L.) under salt stress conditions
Subject Areas : New Finding in Agriculture
1 - M.Sc. Student in Agronomy, Islamic Azad University, Shehr-e-Rey Branch, Shehr-e-Rey, Iran
2 - The Member of Young Researchers and Elit Club, Yadegar-e-Imam Khomeini (RAH) Shahre-rey Branch, Islamic Azad University, Tehran, Iran
Keywords: Salinity, Gibberellin, Salvia (Salvia officinalis L.), Ascorbate,
Abstract :
To investigate the effect of ascorbate and gibberellin on some physiological characteristics of the Salvia medicinal plant under salt stress conditions, an experiment was done at municipal greenhouse No 15 in March 2013. The experiment was as factorial based on completly randomized designe (CRD) with four replications. Experimental factors including salinity levels in 4 levels (0, 25, 50 and 75 mM Nacl), ascorbate in 2 levels (0 and 4 mM) and gibberellin in two levels (0 and 2 mM). The mean comparison showed that the main effect of salinity showed that the cause of the decline of activity of antioxidant enzymes and increase the amount of soluble sugars and proline. . Except of the three double interaction effect of factors on proline and soluble sugars in other cases, there was significant difference on other traits. As well, instead of triple interaction of treatments on, proline, catalase and ascorbate peroxidase in other cases no significant difference was observed. In this case the maximum amount of proline production (12/67 mg/g fw), catalase activity (19/68 mg/protein. min) and ascorbate peroxidase (61/25 mg/protein. min) conducted in 75 mM Nacl and ascorbate and gibberellin application.As a result of the application of ascorbate as a strong antioxidant and GA as growth regulators, foliar damage due to stress, these two compounds were offset and in the absence of ascorbate and GA was given to the plant, the activity of antioxidant enzymes decreased.
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