Effect of Cadmium on Catalase (CAT) Antioxidant Enzyme and Dityrosine (D-T) Biochemical Biomarker in some Crop species
Subject Areas : New Finding in AgricultureS. F. Mousavi 1 , D. Habibi 2 , A. Mozafari 3 , N. A. Sajedi 4
1 - Arak Branch, Islamic Azad University, Arak, Iran
2 - Department of Agronomy and Plant Breeding, Karaj Branch, Islamic Azad University, Karaj, Iran
3 - Department of Agronomy and Plant Breeding, Ilam Branch, Islamic Azad University, Ilam, Iran
4 - Department of Agronomy and Plant Breeding, Arak Branch, Islamic Azad University, Arak, Iran
Keywords: antioxidant enzyme, Cadmium, Heavy metal, Crop species, Biochemical Biomarker, Catalas and Ditirozin,
Abstract :
In order to study Effect of Cadmium on Ditirozin (D-T) Biochemical biomarker and Catalas (CAT) Antioxidant Enzyme in some Crop species the some Crop species, an experiment was conducted in experimental Greenhouse of Islamic Azad University of Karaj branch in 2009-2010 year. The studies were carried out in a Factorial experiment based on a randomized complete block design with three replactions. The factors were Cd doses into soil at three levels 0, 40 and 80 (mg Cd/kg.dw soil) and Crop species at three levels, annual Alfalfa (Medicago rigidula), Hairy vetch (Vicia villosa ) and Canola (Barassica napus). Results showed that different Crop species and Cd doses into soil had highly significant effect on all of Experiment characteristics. Highest amount of Ditirozin (D-T) Biochemical Biomarker related to Canola (with 10.99 µMol/g.Fw) and lowest of this trait related to Hairy vetch (with 8.86 µMol/g.Fw). Highest amount of Catalase (CAT) Antioxidant Enzyme related to Alfalfa (with 55.79 U/mg.protein) and lowest of this trait related to Hairy vetch(with 52.77 U/mg.protein). With increase Cd Doses into soil, Ditirozin (D-T) Biomarker content was increased, but Catalas Antioxidant Enzyme was decreased. 80 (mg Cd/kg.dw Soil) doses with 11.72 (µMol/g.Fw) and 0 (mg Cd/kg.dw Soil) doses with 6.52 (µMol/g.Fw) were highest and lowest Ditirozin (D-T) Content, respectively. 0 and 80 (mg Cd/kg.dw Soil) doses with 63.30 (U/mg.protein) and 49.39 (U/mg.protein) were highest and lowest Catalas (CAT) Antioxidant Enzyme Content, respectively.
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