Comparative HPLC, GC-MS Analysis and In vitro Antifungal Activity of Walnut Kernels against Alternaria mali in Apple
Subject Areas : Plant Pathology
Ruhee Jan
1
,
Tabassum Ara
2
,
Javid Mir
3
1 - Department of Chemistry NIT- Srinagar, JK, India
2 - Department of Chemistry NIT- Srinagar, JK, India
3 - ICAR-Central Institute of Temperate Horticulture, Srinagar, 191132, India
Keywords: Juglans regia, Quercetin, phenolic compounds, Bio-fungicide,
Abstract :
Natural plant products are suitable, eco-friendly, and toxicologically safe for using in integrated disease management approaches. Present work was carried out to decipher the potential of walnut kernels against Alternaria mali, the causal agent of leaf and fruit blotch on apples. Bioactive compound profiling of extracts was also done through HPLC and GC-MS to reveal the potential role of the compounds for disease management. In the present study, a comparative analysis of the anti-fungal activity and concentration of active substances using HPLC and GC-MS in different walnut extracts has been carried out on the inhibition of A. mali. The extracts of walnut kernels belonging to different eco-geographical regions viz. CS (Char-e-Sharief), KG (Kulgam), TM (Tangmarg), and KW (Kupwara) of Kashmir valley were prepared. The extracts prepared were added to potato dextrose agar (PDA) @ 1000, 2000, and 3000 ppm for evaluation against A. mali. The results revealed the efficacy of the different walnut extracts, with a maximum inhibition of mycelia growth in the range of 60%, 62.2%, and 71.1% at 3000ppm. Among them, the TM (Tangmarg) extract showed the highest inhibition (71.1%). The bioactive compounds were identified by using GC-MS chromatographic technique. The Quercetin content was quantified in the kernels of walnut, collected from four locations in Kashmir valley using HPLC. Among the selected genotypes, the highest Quercetin content was observed in samples collected from TM (Tangmarg) (0.765mg g-1) followed by KW (Kupwara) (0.705 mg g-1). The major phenolic components identified by GC/MS were methyl-7,8-Octadecadienoate, hexadecanoic acid, linoleic acid, epicatechin, and tocopherol. The results of the present study can be utilized further in the development of formulation from walnut kernels for evaluation under field conditions.
Akca Y, Sahin U (2022) Responses of ‘Chandler’ walnut variety grafted onto different rootstocks to salt stress. International Journal of Horticultural Science and Technology. 9(1), 1-13.
Ahad T, Gull A, Nissar J, Masoodi L, Rather AH (2020) Effect of storage temperatures, Packaging materials and storage periods on antioxidant activity and non-enzymatic browning of antioxidant treated walnut kernels. Journal of Food Science and Technology. 57(10), 3556-3563.
Ali-Shtayeh MS, Abu Ghdeib SL (1999) Antifungal activity of plant extracts against dermatophytes. Mycoses 42. 665-672.
Anderson KJ, Teuber SS, Gobeille A, Cremin P, Waterhouse AL, Steinberg FM (2001) Walnut polyphenolics inhibit in-vitro human plasma and LDL oxidation. Journal of Nutrition. 131(11), 2837-2842.
Balouiri M, Sadiki M, Ibnsouda SK (2016) Methods for in vitro evaluating antimicrobial activity: a review. Journal of Pharmaceutical Analysis. 6(2), 71-79.
Chatrabnous N, Yazdani N, Vahdati K (2018a) Determination of nutritional value and oxidative stability of fresh walnut. Journal of Nuts. 9(1), 11-20.
Chatrabnous N, Yazdani N, Tavallali V, Vahdati K (2018b) Preserving quality of fresh walnuts using plant extracts. LWT 91:1-7.
Cheniany M, Ebrahimzadeh H, Vahdati K, Preece J, Masoudinejad A, Mirmasoumi M (2013) Content of different groups of phenolic compounds in microshoots of Juglans regia cultivars and studies on antioxidant activity. Acta Physiologia Plantarum. 35, 443–450
Di Venere D, Gatto MA, Ippolito A, Linsalata V, Cascarano NA, Nigro F, Vanadia S (2011) Activity of extracts from wild edible herbs against postharvest fungal diseases of fruit and vegetables. Journal of Post-harvest Biology and Technology. 61(1), 72-82. https://doi.org/10.1016/j.postharvbio.2011.02.005
Ebrahimi S, Jamei R, Nojoomi F , Zamanian Z (2018) Persian walnut composition and its Importance in human health. International Journal of Enteric Pathogens. 6(1), 3-9.
Farsi M, Fatahi Moghadam MR, Zamani Z, Hassani D (2018) Effects of scion cultivar, rootstock age and hormonal treatment on minigrafting of persian walnut. International Journal of Horticultural Science and Technology. 5(2), 185-197.
Fernández-Agullóa A, Pereira E, Freire MS Valentão P, Andrade PB, González-Álvarez J, Pereira JA (2013) Influence of solvent on the antioxidant and antimicrobial properties of walnut (Juglans regia L.) green husk extracts. Journal of Industrial Crops and Products. 42, 126-132.
Filajdic N, Sutton TB (1991) Identification and distribution of Alternaria mali on apples in North Carolina and susceptibility of different varieties of apples to Alternaria blotch. Journal of Plant Disease. 75, 1045-1048.
Galvez L, Gil-Serna J, García M, Iglesias C and Palmero D (2016) Stemphylium Leaf Blight of Garlic (Allium sativum) in Spain: Taxonomy and In Vitro Fungicide Response. The Plant Pathology Journal. 32(5), 388-395.
Ghassan Al-Samarrai, Harbant Singh, Muhammad Syarhabil (2012) Evaluating eco-friendly botanicals (natural plant extracts) as alternatives to synthetic fungicides. Annals of Agricultural and Environmental Medicine. 19(4), 673-676.
Ghorbani N, Moradi H, Kanani M, Ashnavar M (2022) Total flavonoids and phenolic compounds of english daisy
(Bellis Perennis L.) affected by foliar application of nanophosphorus fertilizers. International Journal of Horticultural Science and Technology. 9(4), 405-414.
Grace MH, Yousuf GG, Gustafson SJ, Troung VD, Yencho GC, Lila MA (2014) Phytochemical changes in phenolics, anthocyanins, ascorbic acid and carotenoids associated with sweet potato storage and impacts on bioactive properties. Food Chemistry journal. 145, 717-724.
Habibi A, Yazdani N, Chatrabnous N, Koushesh Saba M, Vahdati K (2022) Inhibition of browning via aqueous gel solution of Aloe vera: a new method for preserving fresh fruits as a case study on fresh kernels of Persian walnut. Journal of Food Science and Technology 59, 2784–2793.
Habibie A, Yazdani N, Saba MK, Vahdati K (2019) Ascorbic acid incorporated with walnut green husk extract for preserving the postharvest quality of cold storage fresh walnut kernels. Scientia Horticulturae 245, 193-199.
Habibi A, Yazdani N, Koushesh Saba M, Vahdati K (2021) Ascorbic acid preserved phenolic compounds of cold stressed fresh walnut kernels. Acta Horticulturae 1315, 665-668.
Habibi A, Yazdani N, Koushesh Saba M, Chatrabnous N, Molassiotis A, Sarikhani S, Vahdati K (2023) Natural preservation and improving lipid oxidation inhibition of fresh walnut. Horticulture, Environment, and Biotechnology. 64, 133–142. https://doi.org/10.1007/s13580-022-00461-3
Hassanein NM, AbouZeid MA, Youssef KA, Mahmoud DA (2008) Efficacy of leaf extracts of neem (Azadirachta indica) and chinaberry (Melia azadirachta) against early blight and wilt diseases of tomato. Australian Journal of Basic and Applied Sciences. 2(3), 763-772.
Hassankhah A, Vahdati K, Rahemi M, Hassani D, Sarikhani Khorami S (2017) Persian walnut phenology: effect of chilling and heat requirements on budbreak and flowering date. International Journal of Horticultural Science and Technology. 4(2), 259-271.
Jahanbani R, Bahramnejad E, Rahimi N, Shafaroodi H, Sheibani N, Moosavi-Movahedi AA, Dehpour A, Vahdati K (2021). Anti-seizure effects of walnut peptides in mouse models of induced seizure: The involvement of GABA and nitric oxide pathways. Epilepsy Research, p.106727.
Jahanbani R, Ghaffari SM, Salami M, Vahdati K, Sepehri H, Sarvestani NN, Sheibani N, Moosavi-Movahedi AA (2016) Antioxidant and anticancer activities of walnut (Juglans regia L.) Protein hydrolysates using different proteases. Plant Foods for Human Nutrition. 71, 402–409. doi: 10.1007/s11130-016-0576-z
Jahanbani R, Ghaffari M, Vahdati K, Salami M, Khalesi M, Sheibani N, Moosavi-Movahedi AA (2018) Kinetics study of protein hydrolysis and inhibition of angiotensin converting enzyme by peptides hydrolysate extracted from walnut. International Journal of Peptide Research and Therapeutics. 24, 77-85.
Johnston A, Booth C (1983) Plant Pathologists Pocket book. 2nd ed. Common wealth Mycological Institute, Kew, Surrey, UK. 24-27.
Kalogiouri NP, Samanidou VF (2021) HPLC fingerprints for the characterisation of walnuts and the detection of fraudulent incidents. Foods. 10, 2145.
Khodadadi F, Tohidfar M, Mohayeji M, Dandekar AM, Leslie CA, Kluepfel D, Butterfield T, Vahdati K (2016) Induction of polyphenol oxidase in walnut and its relationship to the pathogenic response to bacterial blight. Journal of the American Society for Horticultural Science. 141(2), 119-124.
Khodadadi F, Tohidfar M, Vahdati K, Dandekar AM, Leslie CA. (2020). Functional analysis of walnut polyphenol oxidase gene (JrPPO1) in transgenic tobacco plants and PPO induction in response to walnut bacterial blight. Plant Pathology. 69, 756-764.
Kota R, Kordali S, Cakir A, Kesdek M, Kaya, Kilic H (2008) Antimicrobial and insecticidal activities of essential oil isolated from Turkish Salvia hydrangea DC. ex. Benth. Biochemical Systematics and Ecology. 36, (5-6) 360-368.
Latha P, Anand T, Ragupathi N, Prakasam V, Samiyappan R (2009) Antimicrobial activity of plant extracts and induction of systemic resistance in tomato plants by mixtures of PGPR strains and Zimmu leaf extract against Alternaria solani. Journal of Biological Control. 50(2), 85-93.
Li X, Kong W, Shi W, Shen Q (2016) A combination of chemometric methods and GC-MS for the classification of edible vegetable oils. Chemometrics and intelligent laboratory system. 155, 145-150.
Ma Y, Wang C, Liu C, Tan J, Ma H, Wang J (2021) Physiochemical Responses of the Kernel Quality, Total Phenols and antioxidant enzymes of walnut in different forms to the low-temperature storage. Foods. 10(9), 2027.doi: 10.3390/foods10092027
MacDonald H (2000) Conjugated Linoleic acid and Disease Prevention: A Review of current knowledge. Journal of the American College of Nutrition. 19, 111S-118S.
Mate GD, Deshmukh VV, Jiotode DJ, Chore NS, Dikkar M (2005) Efficacy of plant products and fungicides on tomato early blight caused by Alternaria solani. Research on Crops journal. 6(2), 349-351.5.
Milind P, Deepa K (2011) Clove: A champion spice. International journal of research in ayurveda and pharmacy. 2(1), 47-54.
Mishra AK, Mishra A, Kehri HK, Sharma B, Pandey AK (2009) Inhibitory activity of Indian spice plant Cinnamomum zeylanicum extracts against Alternaria solani and Curvularia lunata, the pathogenic dematiaceous moulds. Annals of Clinical Microbiology and Antimicrobials. 8 - 9.
Mohidin FA, Nabi SU (2019) Natural Plant Products: Eco-friendly approach for the management of plant diseases. Eco-friendly agriculture enhancing crop productivity. 212-219.
Oleszek M, Pecio Ł, Kozachok S, Lachowska-Filipiuk Ż, Oszust K, Frąc M (2019) Phytochemicals of apple pomace as prospect bio-fungicide agents against Mycotoxigenic Fungal Species In-Vitro Experiments. Toxins (Basel). 11(6), 361.
Oliveira I, Sousa A, Ferreira ICFR., Bento A, Estevinho L, Pereira JA (2008) Total phenols, antioxidant potential and antimicrobial activity of walnut (Juglans regia L.) green husks. Journal of Food and Chemical Toxicology. 46(7), 2326-2331.
Pan SB, Mukherjee A, Ganguly SB, Mitra A, Bhattacharya A (1985) Antifungal activity of some naturally occurring flavanoids. Zur Pflanzenzucht. 90(4), 392-395.
Parvez MM, Tomita-Yokotani K,Fujii Y, Konishi T, Iwashina T (2004) Effects of quercetin and its seven derivatives on the growth of Arabidopsis thaliana and Neurospora crassa. Biochemical Systematics and Ecology. 3, 631-635.
Reguerio J, Sanchez-Gonzalez C, Vallvendue-Queralt A, Simul-Gandara J, Lamuela-Raventos R, Izquierdo-Pulido M (2014) Comprehensive identification of walnut polyphenols by liquid chromatography coupled to linear ion trap- orbit trap mass spectrometry. Food Chemistry. 152, 340-348.
Sarikhani S, Vahdati K, Ligterink W (2021). Biochemical properties of superior persian walnut genotypes originated from southwest of Iran. International Journal of Horticultural Science and Technology. 8(1), 13-24.
Şesan TE, Enache E, Iacomi B, Oprea M, Oancea F, Iacomi C (2015) Antifungal activity of some plant extracts against Botrytis cinerea Pers. in the blackcurrant CROP (Ribes nigrum L.). Acta Scientiarum Polonorum, Hortorum Cultus. 14, 29-43.
Skadhauge B, Thomsen K, von Wettstein D (1997) The role of barley testa layer and its flavonoid content in resistance to Fusarium infections. Hereditas. 126, 147-160.
Soleimani MJ, Esmailzadeh M (2007) First report of Alternaria mali causing apple leaf blotch disease in Iran. Australasian Plant Disease Notes. 2, 57-58. https:// doi. org/10. 1071/DN07023
Stampar F, Solar A, Hudina M, Veberic R, Colaric M (2006) Traditional walnut liqueur – cocktail of phenolics. Food Chemistry. 95(4), 627-631.
Stangarlin JR, Kuhn JO, Assi L, Schwan-Estrada KRF (2011) Control of plant diseases using extracts from medicinal plants and fungi. Science against microbial pathogens: communicating current research and technological advances. Antonio Méndez-Vilas (Ed.). 1, 1033-1042.
Tian Y, Xu Z, Zheng B, Martin Lo Y (2013) Optimisation of ultrasonic-assisted extraction of pomegranate (Punica granatum L.) seed oil. Ultrasonics sonochemistry. 20(1), 202-208.
Vahdati K (2000) Walnut situation in Iran. Nucis Newsletter. 9, 32-33.
Wianowska D, Garbaczewska S, Cieniecka- Roslonkiewicz A, Dawidowicz A (2016) Comparison of antifungal activity of extracts from different Juglans regia cultivars and juglone. Microbial Pathogenesis. 100, 263-267.
Zaker M, Mosallanejad H (2010) Antifungal activity of some plant extracts on Alternaria alternata, the causal agent of alternaria leaf spot of potato. Pakistan Journal of Biological Sciences. 13(21), 1023-9.
