the effect of Fe oxide nano particles to prevention Fe chlorosis ofrootstock (citrus aurantium) in vitro condition
Subject Areas : Journal of Plant Ecophysiology
soad saeedi
1
(Graduated of the Shahid Chamran University)
Mousa Mousavi
2
(Assistant Professor, Shahid Chamran University)
Mohamad hadi ghaffarian mogharab
3
(مرکز تحقیقات کشاورزی و منابع طبیعی زنجان، زنجان، ایران)
Keywords: Tissue culture, iron deficiency, Iron chelate, Citrus,
Abstract :
Iron deficiencies mostly appears as chlorosis in young leaves as one of the most important nutritional disorders is citrus trees and severely affect yield and quality of their crop. Suitable selection of iron composition is one of important solution ways for prevent of this nutritional question. For this purpose experiment to investigate the effect of iron composition type including of FeEDTA, FeEDDHA and two type of iron oxide nanoparticles uncoated and coated with polyethylene glycol for prevention iron chlorosis in sour orange (Citrus aurantium) rootstock on Murashige and Skoog (MS) medium without iron in completely randomized design with five replicates was followed. The sour orange seeds were cultivated on MS medium with different iron composition .At the end of experimental period (2 months) vegetative characteristics (root, shoot and seedling length, number and leave area), seedling, root and shoot dry and fresh weights, the amount of chlorophyll a, b and total, Leaf iron concentration and chlorosis percentage, were measured. The results indicated that MS culture medium with uncoated nanoparticles of iron oxide had highest significant effect at <1% probability on increase of iron uptake and the lowest degree of chlorosis of seedlings were grown on culture medium. It was also observed that MS medium with iron nanoparticles have significant effects on increase of vegetative characteristics, fresh and dry weights and the chlorophyll content than control (MS without iron chelated).
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