Evaluation of Antioxidant and Antimicrobial Activities of Methanolic Extract from Malva silvestans and Lowsonia inermis on intestinal bactria

Subject Areas : Medical Microbiology

Zahra Hojjati Bonab ¹ , Elhameh Nik khah ²

1 - Department of Microbiology, Bonab Branch, Islamic Azad University, Bonab, Iran
2 - Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran

Received: 2013-04-14 Accepted : 2013-04-14 Published : 2010-10-01

Keywords: Antioxidant, Antibacterial, Free Radicals, Enteric Bacteria,

Abstract :

Background and Objectives: Malva silvestans and Lawsonia inermis are widely used in the East Azerbaijan folk medicine. The effective compounds of these plants describe probabely their biological activities and therapeutic activity. In this study, methanolic extract of these plants were used to detect antioxidant and antimicrobial activity. Material and Methods: Superoxide anion radical scavenging assay was used for antioxidant activity test in which the superoxide anion radicals were generated by a pyrogallol auto oxidation system. The antimicrobial activity of methanolic extract of these plants was tested by disc diffusion method against three enteric microorganisms. The minimal inhibitory concentrations (MICs) of methanolic extracts were determined, too. Results: The used assay for assessment of antioxidant activity in order to determine the accumulation of superoxide radicals in total extract of plant was not appropriate. However, for antibacterial assays, all three bacteria were susceptible to methanolic extract and showed 4 to 15 mm zone of inhibition. Conclusion: Although the total extracts of plants used in this study had appropriate antimicrobial activities,their  antioxidant effects was not significant.

References:

1. Oussalah M, Caillet S, Saucier L, Lacroix M. Inhibitory effects of selected plant essential oils on the growth of four pathogenic bacteria: E. coli O157: H7, Salmonella typhimurium, Staphylococcus aureus and Listeria monocytogenes. Food Control. 2007; 18 (5): 414-20.

2. Aruoma OI. Free radicals, oxidative stress and antioxidants in human health and disease. J Am Oil Chem Soc. 1998; 75(2): 199-212.

 3. Block G, Langseth L. Antioxidant vitamins and disease prevention. Food Technol. 1994; 48(7): 80–84.

4. Hertog MGL, Feskens EJM, Hollman PCH, Katan MB, Kromhout D.  Dietary antioxidant flavonoids and risk of coronary heart disease: The Zutphen elderly study. Lancet. 1993; 342(8878): 1007–1011.

5. Lee, J Koo, N Min DB. Reactive oxygen species, aging, and antioxidative nutraceuticals. Compr Rev Food Sci Food Saf. 2004; 3(1): 21–33.

6. Zhonggao C, Felgines O, Texier C, Besson D Jiao, Liu J, Wang S. Antioxidant activities of total pigment extract from blackberries. Food Technol Biotechnol. 2005; 43(1): 97–102.

7. Nikkhah E, Khayami M, Heidari R. Evaluation of nitric oxide scavenging activity of anthocyanins from blackberry (Morus nigra), strawberry (Fragaria vesca) and berry (Morus alba Var, Nigra) extracts. Iran J Med Arom Plants. 2009; 25(1): 120-128.

8. Jalali M, Abedi D, Asghari GH, Rezaee ZA. Study of Anti-Microbial Effect of Pycnocycla Spinosa's Fruit Extracts. J Mazan Uni Med Sci. 2007; 17(59): 76-86. [ In Persian].

9. Walter C, Zabta K, Wari S, Afzal I, Malik RN. Antibacterial activityin herbal products used in Pakistan. Pak J Bot. 2011; 43: 155-162.

10. Pirbalouti AG, Yousefi M, Nazari H, Karimi I, Koohpayeh A. Evaluation of burn healing properties of Arnebia euchorma and Malva sylvestris. Elec J Bio. 2009; 5(3): 62-66.

11. Islam M, Ali E, Asif Saeed M, Jamshaid M, Tahir Javed Khan M. Antimicrobial and irritant activities of the extracts of Malva parviflora L, Malvastrum coromandelianum L, AND Amaranthus viridis L. Pak J Pharm. 2010; 20-23 (1 & 2): 3-6.

12. Razavi SM, Zarrini Gh, Molavi Gh, Ghasemi Gh. Bioactivity of Malva Sylvestris L, a Medicinal Plant from Iran, Iranian Journal of Basic Medical Sciences. 2011; 14(6): 574-579.

13. Gagandeep Ch, Sandeep G, Priyanka P.  Lawsonia inermis Linnaeus: A Phytopharmacological Review. Int J Pharma Sci Drug Res. 2010; 2(2): 91-98.

14. Arun P, Purushotham KG, Johnsy jayarani J, kumaraV. In vitro antibacterial activity and flavonoid contents of Lawsonia inermis (Henna). Int J PharmTech Res. 2010;  2(2): 1178-1181.

15. Habbal O, Hasson SS, El-Hag AH, Al-Mahrooqi Z, Al-Hashmi N, Al-Bimani Z, Al-Balushi MS, Al-Jabri AA. Antibacterial activity of Lawsonia inermis Linn (Henna) against Pseudomonas aeruginosa. Asi Pac J Trop Biomed. 2011; 1(3): 173-176.

16. Saadabi A. Evaluation of lawsonia inermis Linn, (soudanese henna) leaf extracts as an antimicrobial agent. Res J Biol Sci. 2007; 2(4): 419-423.

17. Ferdous AJ, Islam N, Faroque ABM, Ahsan M. In vitro testing of the leaf extracts of Lowsonia alba for antimicrobial properties. Pak J Pharma Sci. 1990; 3(2): 75-79.

 Stief TW. The physiology and pharmacology of singlet oxygen. Med Hypotheses. 2003; 60(4): 567–572.

18. Afolayan AJ, Aboyade OM, Sofidiya MO. Total phenolic content and free radical scavenging activity of Malva parviflora L, (Malvaceae). J Biol Sci. 2008; 8(5): 945-949.

19. Khodaparast H, Hosein M, Dezashibi Z. Antioxidant activity of Henna leaves extracts (Lawsonia inermis).World J Dairy Food Sci. 2007; 2 (1): 38-41.

20. Ling LT, Radhakrishnan AK, Subramaniam TH, Cheng MH,  Palanisamy UD. Assessment of Antioxidant Capacity and Cytotoxicity of Selected Malaysian Plants. Molecules. 2010; 15(4): 2139-2151.

21. Zhonggao C, Felgines O, Texier C, Besson DJ, Liu J, Wang S. Antioxidant Activities of Total Pigment Extract from Blackberries. Food Technol Biotechnol. 2005; 43(1): 97–102.

22. Li CM, Xie BJ. Investigation with spectrophotomeric method on the radical scavenging effect of tea polyphenol and its oxidant. Fine Chemicals. 2000; 17: 241–244.

23. Zhang WJ, Ge C. Comparation of pyrogallol autoxida-tion and NBT photoreduction assay in detecting activity of SOD. Journal of Hebei Vocation-Technical Teachers College. 2000; 14: 68–70.