Association between IL-12 Receptor beta-1 gene polymorphisms and Tuberculosis between Iranian Population

Subject Areas : Molecular Microbiology

Noor olhoda Saadaee Jahromi ¹ , Parissa Farnia ² , Mohammad Kargar ³ , Mehdi Kazem pour ⁴ , Jamileh Nowroozi ⁵ , Mohammad Reza Masjedi ⁶ , Aliakbar Velayati ⁷

1 - Department of Microbiology, Islamic Azad University, Jahrom Branch, Jahrom, Iran
2 - Mycobacterium Research Center (MRC), National Research Institute Of Tuberculosis and Lung Disease (NRITLD)
3 - Department of Microbiology, Islamic Azad University, Jahrom Branch, Jahrom, Iran
4 - Mycobacterium Research Center (MRC), National Research Institute Of Tuberculosis and Lung Disease (NRITLD)
5 - Department of Microbiology, Shahid Beheshti University of Medical sciences, Tehran, Iran
6 - Mycobacterium Research Center (MRC), National Research Institute Of Tuberculosis and Lung Disease (NRITLD)
7 - Mycobacterium Research Center (MRC), National Research Institute Of Tuberculosis and Lung Disease (NRITLD)

Received: 2009-06-15 Accepted : 2009-08-16 Published : 2009-07-01

Keywords: Polymorphism, Interleukin 12, Mycobacteium tuberculosis, Beta-1 receptor,

Abstract :

Background and objectives: Tuberculosis (TB) is the most common cause of death due to infectious disease, worldwide. In fact only 10% of people infected with Mycobacterium tuberculosis develop clinical disease which suggests , the role of host  genetic factors in susceptibility  against TB. For this reason, we aimed to determined IL-12 gene polymorphism among  PTB cases. Material and method: A case-control study was carried out in one hundred twenty TB patient  and one hundred sixty seven healthy control individuals. The Single Nucleotide Polymorphism (SNP ) in 5 regions of IL12 RB1 gene +705A/G  +1158T/C, +1196G/C,+1664C/T and +1637 G/A  were determinate by PCR-RFLP. Results: No significance differences were detected at +1664 C/T and +1637 G/A allele frequencies. Conclusion:  In this study, the IL-12 gene polymorphism at +1664 C/T and +1637G/A allele were associated with susceptibility against TB. Although, further studies suggested on more number of samples.