An analysis for assay role of Nonpolio Entreviruses for cause of AFP in Iran, by five cell lines

Subject Areas : Medical Microbiology

Firouz Abbasian ¹ , Hamideh Tabatabaei ² , Mahboubeh Sarijlou ³ , Rakhshande Nategh ⁴

1 - Department of Microbiology Tobnkabon Branch, Islamic Azad University, Tonkabon, Iran
2 - Department pf Virology, School of Public Health Tehran University, Tehran, Iran
3 - Department pf Virology, School of Public Health Tehran University, Tehran, Iran
4 - Department pf Virology, School of Public Health Tehran University, Tehran, Iran

Received: 2008-11-15 Accepted : 2009-01-14 Published : 2009-01-01

Keywords: Acute Flaccid Paralysis, Cell line, Nonpolio Entroviruses,

Abstract :

Background and objectives: AFP (Acute flaccid paralysis) is a suddenly weakness of any organs of body that its symptoms reach to peak after 4-5 day, but it don't progress. AFP has much etiology that Poliovirus is more important than others. At currently, Poliovirus has been eradicated in most countries, and then we must analysis role of nonpolio agent in this disease. Material and Methods: In this work, all of stool specimens of AFP patient (2003) inoculate to five cell line (L20B, RD, Hep-2, Vero and GMK). And have evaluted with micronutrilization tests. Results: We inoculate two specimens from any 460 case fewer than 15 years AFP patient to these five lines. Ultimately, we isolated 34 different species and serotypes of Entroviruses. Upon this result, 7.1% of all cases were positive for cell culture and isolation of Entroviruses. Sexuality doesn't have an effective role in isolation of Entroviruses. Most of these viruses isolated from less than 0-5 year's children and it decline by increase in age. Echoviruses (12 cases) and Polioviruses (12 cases) have been isolated more than others. Conclusion: We showed a meaningful difference between RP (Residual Paralysis) positive and RP negative cases. Upon this result, we showed nonpolio Entroviruses can cause RP. It doesn't have meaningful difference isolation of Vaccine Poliovirus between RP positive and RP negative patient. Then we showed OPV vaccines used in Iran, have enough safety. The difference in isolation of Coxsacie virus between RP positive and RP negative is not meaningful. We demonstrate Echoviruses can cause AFP in patient fewer 5 years, because the difference in isolation of Echoviruses between RP positive and RP negative patient is meaningful.