Optimization of fatty acids produced by Candida glabrata isolated frome soil
Subject Areas :
Applied Microbiology
Elahe Tajedini
1
,
Mahboobeh Madani
2
,
Masoud Fouladgar
3
,
Rasoul Mohammadi
4
,
Ali Zarei Mahmoudabadi
5
1 - Department of Microbiology, Falavarjan Branch, Islamic Azad University, Isfahan, Iran
2 - Department of Microbiology, Falavarjan Branch, Islamic Azad University, Isfahan, Iran
3 - Department of Biochemistry, Falavarjan Branch, Islamic Azad University, Isfahan, Iran.
4 - Department of Medical Parasitology and Mycology, Infectious Diseases and Tropical Medicine Research Center, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
5 - Department of Medical Mycoparasitology, School of Medicine, Ahvaz Jundishapour University of Medical Sciences, Ahvaz, Iran
Received: 2022-09-10
Accepted : 2023-02-04
Published : 2023-03-06
Keywords:
Fatty Acids,
GC-MS,
Candida glabrata,
Yeast,
microbial species,
Abstract :
Background & Objectives: Oils extracted from microorganisms (SCO) are preferable to vegetable oils due to containing more gamma-linolenic acid, more stability against oxidation, and less content of residual pesticides. The oils can be used as dietary supplements. The aim of this study was molecular identification of lipid-producing yeasts and molds in soil and optimization of fat production by Candida glabrata using the Taguchi design.Materials & Methods: Several yeasts and molds were isolated from the soil samples of groves and near oil change shops and restaurants. Molecular identification was performed by polymerase chain reaction (PCR). Among them, the highest lipid producers were selected and using the Taguchi design. The best conditions in terms of carbon and nitrogen sources as well as pH for maximum lipid production were determined. The fat obtained was examined by gas chromatography with a mass spectrometer detector (GC-MS).Results: Among the identified species, C. glabrata had the highest lipid production. Production of palmitoleic, palmitic, linoleic, linolenic, and stearic acid was proven in this study. Lipid production in C. glabrata and Mortierella alpina was 6.9 and10.8 grams per litre, respectively.Conclusion: Due to the rapid reproduction in yeasts and their ability to produce fatty acids, C. glabrata is a suitable option for fat production.
References:
1. Madani M, Enshaeieh M, Abdoli A. Single cell oil and its application for biodiesel production. Process Saf Environ Prot. 2017; 111:560-747.
2. Bento HB, Carvalho AK, Reis CE, De Castro HF. Single cell oil production and modification for fuel and food applications: assessing the potential of sugarcane molasses as culture medium for filamentous fungus. Ind Crops Prod. 2020; 145:113-141.
3. Taati M, Habibi Rezaei M, Keihan A H. Omega-3 Fatty Acids: Anti-Inflammatory Effects to Ensure the Health. J Mar Med. 2020; 2 (3):135-149.
4. Christie WW, Harwood JL. Oxidation of polyunsaturated fatty acids to produce lipid mediators. Essays Biochem. 2020; 64(3):401-21.
5. Konur O. Algal biomass production for biodiesel production: A review of the research. Biodiesel Fuels Based on Edible and Nonedible Feedstocks, Wastes, and Algae. 2021; 5:695-717.
6. Rohr M, Narasimhulu CA, Hamid S, Parthasarathy S. The dietary peroxidized lipid, 13-HPODE, promotes intestinal inflammation by mediating granzyme B secretion from natural killer cells. Food Funct. 2020; 11(11):9526-34.
7. Czumaj A, Śledziński T. Biological Role of Unsaturated Fatty Acid Desaturases in Health and Disease. Nutrients. 2020 Jan 29; 12(2):356.
8. Zajonc DM. The CD1 family: serving lipid antigens to T cells since the Mesozoic era. Immunogenetics. 2016; (8):561-76.
9. Rashidi, S., Shahsavandi, S. Immunologic adjuvants: An overview of Toll-like receptors mechanism of action in triggering the immune responses. mjms.2018; 61(3): 1061-1070.
10. Matuła K, Rivello F, Huck WT. Single‐cell analysis using droplet microfluidics. Adv Biosyst. 2020; 4(1):1900188.
11. Zlatanov, M; Pavlova, K; Grigorova, D. Composition of some yeast strains from Livingston Island, Antarctica. Folia Microbiologica. 2001: 46 (5): 402-6.
12. Mhlongo S, Ezeokoli OT, Roopnarain A, Ndaba B, Sekoai PT, Habimana O, Pohl CH. The potential of single-cell oils derived from filamentous fungi as alternative feedstock sources for biodiesel production. Front Microbiol. 2021; 12:57.
13. Sagia S, Sharma A, Singh S, Chaturvedi S, Nain PK, Nain L. Single cell oil production by a novel yeast Trichosporon mycotoxinivorans for complete and ecofriendly valorization of paddy straw. Electron J Biotechnol. 2020; 1:44:60-8.
14. Frederiks WM, Slob A, Frank JJ, Tas J. (Histo)chemical aspects of Sudan Black B in various solvents. Acta Histochem Suppl. 1981; 24:259-65. PMID: 6785833
15. Pan LX, Yang DF, Shao L, Li W, Chen GG, Liang ZQ. Isolation of the oleaginous yeasts from the soil and studies of their lipid-producing capacities. Food Technol Biotechnol. 2009; 47(2):215-20.
16. Manirakiza P, Covaci A, Schepens P. Comparative study on total lipid determination using Soxhlet, Roese-Gottlieb, Bligh & Dyer, and modified Bligh & Dyer extraction methods. J Food Compos Anal. 2001; 14(1):93-100.
17. Leite LN, Lelis FJ, de Sousa Xavier MA, dos Santos J, Cardoso L, Barbosa FS, dos Santos RF, Dias SA, de Oliveira Xavier AR. Molecular identification and characterization of filamentous fungi and yeasts isolated in a pharmaceutical industry environment. J Appl Pharm Sci. 2020 Jul; 10(7): 27-37.
18. Castoria R, Miccoli C, Barone G, Palmieri D, De Curtis F, Lima G, Heitman J, Ianiri G. Molecular Tools for the Yeast Papiliotrema terrestris LS28 and Identification of Yap1 as a Transcription Factor Involved in Biocontrol Activity. Appl Environ Microbiol. 2021; 87(7):e02910-20.
19. Mahmoudi SB, Saffarian Abbaszadeh M, Abbasi S, Farrokhinejad R. Genetic diversity and pathogenic variability among Cercospora beticola Sacc. Isolates causing leaf spot of sugar beet in Iran. J Crop Prot. 2018; 7 (2): 207-217.
20. Ghanavati H, Abdoli A, Nahvi I, Enshaeieh M, Madani M. Application of statistical processes in improving lipid production by native oleaginous yeast Rhodotorula spp. strain Yr2. ABJ. 2013; 5(1): 129-144.
21. Gui -you L, Sheng SY, Dai C. Factors affecting γ-linolenic acid content in fermented glutinous rice brewed by Rhizopus sp. Food Microbiol. 2004:21(3):299-304.
22. Miao X, Wu Q. Biodiesel production from heterotrophic microalgal oil. Bioresour Technol. 2006; 97(6):841-6
22. Dai C, Tao J, Xie F, Dai Y, Zhao M. Biodiesel generation from oleaginous yeast Rhodotorula glutinis with xylose assimilating capacity. Afr J Biotechnol. 2007; 6(17): 2130-2134.
23. Li Y, Ghasemi Naghdi F, Garg S, Adarme-Vega TC, Thurecht KJ, Ghafor WA, Tannock S, Schenk PM. A comparative study: the impact of different lipid extraction methods on current microalgal lipid research. Microb Cell Fact. 2014; 13:14.
24. Homayouni-rad A, Oroojzadeh P, Abbasi A. The Effect of Yeast Kluyveromyces marxianus as a Probiotic on the Microbiological and Sensorial Properties of Set Yoghurt during Refrigerated Storage. J Ardabil Univ Med Sci. 2021; 20 (2):254-268.
25. Enshaeieh M, Abdoli A, Nahvi I, Madani M. Production and optimization of microbial oil from oleaginous yeasts Yarrowia lipolytica DSM & native yeast Geotrichum Bl. NCMBJ. 2014; 3 (12):57-64.
26. Bandhu S, Srivastava A, Ghosh D, Chaudhuri TK. Yeast Single Cell Oils from Bioresources: Current Developments in Production and Applications. Current Sustainable/Renewable Energy Reports. 2020 Oct 7:1-2.
27. Ghasemi L, Samadlouie HR, Jalali H, Gharanjik SH. Isolation and identification of Candida Orthopsilosi SAGSGC as oleaginous yeast in perch fish by using ribosomal gene and optimization of oil and biomass production. JFST. 2017; 70(14): 1-12.
28. Gema H, Kavadia A, Dimou D, Tsagou V, Komaitis M, Aggelis G. Production of γ-linolenic acid by Cunninghamella echinulata cultivated on glucose and orange peel. Microbiol Biotechnol. 2002; 58(2002), 303–307.
29. Liszkowska W, Berlowska J. Yeast Fermentation at Low Temperatures: Adaptation to Changing Environmental Conditions and Formation of Volatile Compounds. Molecules. 2021 Jan; 26(4):1035.
30. Matsuzawa T, Maehara T, Kamisaka Y, Ayabe-Chujo Y, Takaku H, Yaoi K. Identification and characterization of Pseudozyma antarctica Δ12 fatty acid desaturase and its utilization for the production of polyunsaturated fatty acids. J Biosci Bioeng. 2020; 130(6):604-609.
31. Nwokoro O. Effects of the pH on the growth, lipid and fatty acid production of Candida utilis and Candida tropicalis grown in cane molasses. Ann. Food Sci Technol. 2018; 19:128-34
32. Ngamsirisomsakul M, Reungsang A, Kongkeitkajorn MB. Assessing oleaginous yeasts for their potentials on microbial lipid production from sugarcane bagasse and the effects of physical changes on lipid production. Bioresource Technology Reports. 2021; 14:100650.
33. Nasirian N, Levin D. Evaluation of In Vitro Lipid and Carotenoid Synthesis by Yeast, Rhodosporidium Diobovatum. Iranian J Nutr Sci Food Technol. 2018; 13 (1): 85-94.
34. Berikten D, Hoşgün EZ, Otuzbiroğlu AG, Bozan B, Kıvanç M. Lipid Production from Crude Glycerol by Newly Isolated Oleaginous Yeasts: Strain Selection, Molecular Identification and Fatty Acid Analysis. Waste and Biomass Valor. 2021; 12: 5461–5470.
35. Thangavelu K, Sundararaju P, Srinivasan N, Muniraj I, Uthandi S. Simultaneous lipid production for biodiesel feedstock and decontamination of sago processing wastewater using Candida tropicalis ASY2. Biotechnol Biofuels. 2020; 13(1):1-4.
36. Tang S, Dong Q, Fang Z, Cong WJ, Zhang H. Microbial lipid production from rice straw hydrolysates and recycled pretreated glycerol. Bioresour Technol. 2020; 312:123-580.
Katrin Ochsenreither1, Claudia Glück, Timo Stressler, Lutz Fischer and Christoph Syldatk. Production Strategies and Applications of Microbial Single Cell Oils. Front. Microbio. 2016.volume7.article1539.
Heitor B.S.Bento,Ana K.F.CarvalhoCristiano, E.R.ReisHeizir, F.De Castro. Single cell oil production and modification for fuel and food applications: Assessing the potential of sugarcane molasses asmediumfor filamentus fugus.2020. Industrial Crops and Products .Volume 145, 11241.
Shivani Chaturvedi, Arti Kumari, Amrik Bhatacharya, Anamika Sharma, Lata Nain & Sunil K. Khare . Banana peel waste management for single-cell oil production. 2018. Energy, Ecology and Environment volume 3, pages296–303.
Muammer Demir,Aysen Güher Gündes.. Single-cell oil production by Mortierella isabellina DSM 1414 using different sugars as carbon source.2020. Biotechprog.volum 36.issue6.
Aabid ManzoorShahaHassanMohamedabZichenZhangaYuandaSonga. Isolation, characterization and fatty acid analysis of Gilbertella persicaria DSR1: A potential new source of high value single-cell oil. 2021. Biomass and Bioenergy.Volume151.106156.
Alireza Rasouli, Seyyed Soheil Aghaee, Mohsen Zargar, Single-cell Oil Production Using Low-Cost Carbon
Sources by Newly Isolated Kocuria Y205. 2021. Archive of Hygene Science. Volume 10. Number 2.
_||_
1. Madani M, Enshaeieh M, Abdoli A. Single cell oil and its application for biodiesel production. Process Saf Environ Prot. 2017; 111:560-747.
2. Bento HB, Carvalho AK, Reis CE, De Castro HF. Single cell oil production and modification for fuel and food applications: assessing the potential of sugarcane molasses as culture medium for filamentous fungus. Ind Crops Prod. 2020; 145:113-141.
3. Taati M, Habibi Rezaei M, Keihan A H. Omega-3 Fatty Acids: Anti-Inflammatory Effects to Ensure the Health. J Mar Med. 2020; 2 (3):135-149.
4. Christie WW, Harwood JL. Oxidation of polyunsaturated fatty acids to produce lipid mediators. Essays Biochem. 2020; 64(3):401-21.
5. Konur O. Algal biomass production for biodiesel production: A review of the research. Biodiesel Fuels Based on Edible and Nonedible Feedstocks, Wastes, and Algae. 2021; 5:695-717.
6. Rohr M, Narasimhulu CA, Hamid S, Parthasarathy S. The dietary peroxidized lipid, 13-HPODE, promotes intestinal inflammation by mediating granzyme B secretion from natural killer cells. Food Funct. 2020; 11(11):9526-34.
7. Czumaj A, Śledziński T. Biological Role of Unsaturated Fatty Acid Desaturases in Health and Disease. Nutrients. 2020 Jan 29; 12(2):356.
8. Zajonc DM. The CD1 family: serving lipid antigens to T cells since the Mesozoic era. Immunogenetics. 2016; (8):561-76.
9. Rashidi, S., Shahsavandi, S. Immunologic adjuvants: An overview of Toll-like receptors mechanism of action in triggering the immune responses. mjms.2018; 61(3): 1061-1070.
10. Matuła K, Rivello F, Huck WT. Single‐cell analysis using droplet microfluidics. Adv Biosyst. 2020; 4(1):1900188.
11. Zlatanov, M; Pavlova, K; Grigorova, D. Composition of some yeast strains from Livingston Island, Antarctica. Folia Microbiologica. 2001: 46 (5): 402-6.
12. Mhlongo S, Ezeokoli OT, Roopnarain A, Ndaba B, Sekoai PT, Habimana O, Pohl CH. The potential of single-cell oils derived from filamentous fungi as alternative feedstock sources for biodiesel production. Front Microbiol. 2021; 12:57.
13. Sagia S, Sharma A, Singh S, Chaturvedi S, Nain PK, Nain L. Single cell oil production by a novel yeast Trichosporon mycotoxinivorans for complete and ecofriendly valorization of paddy straw. Electron J Biotechnol. 2020; 1:44:60-8.
14. Frederiks WM, Slob A, Frank JJ, Tas J. (Histo)chemical aspects of Sudan Black B in various solvents. Acta Histochem Suppl. 1981; 24:259-65. PMID: 6785833
15. Pan LX, Yang DF, Shao L, Li W, Chen GG, Liang ZQ. Isolation of the oleaginous yeasts from the soil and studies of their lipid-producing capacities. Food Technol Biotechnol. 2009; 47(2):215-20.
16. Manirakiza P, Covaci A, Schepens P. Comparative study on total lipid determination using Soxhlet, Roese-Gottlieb, Bligh & Dyer, and modified Bligh & Dyer extraction methods. J Food Compos Anal. 2001; 14(1):93-100.
17. Leite LN, Lelis FJ, de Sousa Xavier MA, dos Santos J, Cardoso L, Barbosa FS, dos Santos RF, Dias SA, de Oliveira Xavier AR. Molecular identification and characterization of filamentous fungi and yeasts isolated in a pharmaceutical industry environment. J Appl Pharm Sci. 2020 Jul; 10(7): 27-37.
18. Castoria R, Miccoli C, Barone G, Palmieri D, De Curtis F, Lima G, Heitman J, Ianiri G. Molecular Tools for the Yeast Papiliotrema terrestris LS28 and Identification of Yap1 as a Transcription Factor Involved in Biocontrol Activity. Appl Environ Microbiol. 2021; 87(7):e02910-20.
19. Mahmoudi SB, Saffarian Abbaszadeh M, Abbasi S, Farrokhinejad R. Genetic diversity and pathogenic variability among Cercospora beticola Sacc. Isolates causing leaf spot of sugar beet in Iran. J Crop Prot. 2018; 7 (2): 207-217.
20. Ghanavati H, Abdoli A, Nahvi I, Enshaeieh M, Madani M. Application of statistical processes in improving lipid production by native oleaginous yeast Rhodotorula spp. strain Yr2. ABJ. 2013; 5(1): 129-144.
21. Gui -you L, Sheng SY, Dai C. Factors affecting γ-linolenic acid content in fermented glutinous rice brewed by Rhizopus sp. Food Microbiol. 2004:21(3):299-304.
22. Miao X, Wu Q. Biodiesel production from heterotrophic microalgal oil. Bioresour Technol. 2006; 97(6):841-6
22. Dai C, Tao J, Xie F, Dai Y, Zhao M. Biodiesel generation from oleaginous yeast Rhodotorula glutinis with xylose assimilating capacity. Afr J Biotechnol. 2007; 6(17): 2130-2134.
23. Li Y, Ghasemi Naghdi F, Garg S, Adarme-Vega TC, Thurecht KJ, Ghafor WA, Tannock S, Schenk PM. A comparative study: the impact of different lipid extraction methods on current microalgal lipid research. Microb Cell Fact. 2014; 13:14.
24. Homayouni-rad A, Oroojzadeh P, Abbasi A. The Effect of Yeast Kluyveromyces marxianus as a Probiotic on the Microbiological and Sensorial Properties of Set Yoghurt during Refrigerated Storage. J Ardabil Univ Med Sci. 2021; 20 (2):254-268.
25. Enshaeieh M, Abdoli A, Nahvi I, Madani M. Production and optimization of microbial oil from oleaginous yeasts Yarrowia lipolytica DSM & native yeast Geotrichum Bl. NCMBJ. 2014; 3 (12):57-64.
26. Bandhu S, Srivastava A, Ghosh D, Chaudhuri TK. Yeast Single Cell Oils from Bioresources: Current Developments in Production and Applications. Current Sustainable/Renewable Energy Reports. 2020 Oct 7:1-2.
27. Ghasemi L, Samadlouie HR, Jalali H, Gharanjik SH. Isolation and identification of Candida Orthopsilosi SAGSGC as oleaginous yeast in perch fish by using ribosomal gene and optimization of oil and biomass production. JFST. 2017; 70(14): 1-12.
28. Gema H, Kavadia A, Dimou D, Tsagou V, Komaitis M, Aggelis G. Production of γ-linolenic acid by Cunninghamella echinulata cultivated on glucose and orange peel. Microbiol Biotechnol. 2002; 58(2002), 303–307.
29. Liszkowska W, Berlowska J. Yeast Fermentation at Low Temperatures: Adaptation to Changing Environmental Conditions and Formation of Volatile Compounds. Molecules. 2021 Jan; 26(4):1035.
30. Matsuzawa T, Maehara T, Kamisaka Y, Ayabe-Chujo Y, Takaku H, Yaoi K. Identification and characterization of Pseudozyma antarctica Δ12 fatty acid desaturase and its utilization for the production of polyunsaturated fatty acids. J Biosci Bioeng. 2020; 130(6):604-609.
31. Nwokoro O. Effects of the pH on the growth, lipid and fatty acid production of Candida utilis and Candida tropicalis grown in cane molasses. Ann. Food Sci Technol. 2018; 19:128-34
32. Ngamsirisomsakul M, Reungsang A, Kongkeitkajorn MB. Assessing oleaginous yeasts for their potentials on microbial lipid production from sugarcane bagasse and the effects of physical changes on lipid production. Bioresource Technology Reports. 2021; 14:100650.
33. Nasirian N, Levin D. Evaluation of In Vitro Lipid and Carotenoid Synthesis by Yeast, Rhodosporidium Diobovatum. Iranian J Nutr Sci Food Technol. 2018; 13 (1): 85-94.
34. Berikten D, Hoşgün EZ, Otuzbiroğlu AG, Bozan B, Kıvanç M. Lipid Production from Crude Glycerol by Newly Isolated Oleaginous Yeasts: Strain Selection, Molecular Identification and Fatty Acid Analysis. Waste and Biomass Valor. 2021; 12: 5461–5470.
35. Thangavelu K, Sundararaju P, Srinivasan N, Muniraj I, Uthandi S. Simultaneous lipid production for biodiesel feedstock and decontamination of sago processing wastewater using Candida tropicalis ASY2. Biotechnol Biofuels. 2020; 13(1):1-4.
36. Tang S, Dong Q, Fang Z, Cong WJ, Zhang H. Microbial lipid production from rice straw hydrolysates and recycled pretreated glycerol. Bioresour Technol. 2020; 312:123-580.
Katrin Ochsenreither1, Claudia Glück, Timo Stressler, Lutz Fischer and Christoph Syldatk. Production Strategies and Applications of Microbial Single Cell Oils. Front. Microbio. 2016.volume7.article1539.
Heitor B.S.Bento,Ana K.F.CarvalhoCristiano, E.R.ReisHeizir, F.De Castro. Single cell oil production and modification for fuel and food applications: Assessing the potential of sugarcane molasses asmediumfor filamentus fugus.2020. Industrial Crops and Products .Volume 145, 11241.
Shivani Chaturvedi, Arti Kumari, Amrik Bhatacharya, Anamika Sharma, Lata Nain & Sunil K. Khare . Banana peel waste management for single-cell oil production. 2018. Energy, Ecology and Environment volume 3, pages296–303.
Muammer Demir,Aysen Güher Gündes.. Single-cell oil production by Mortierella isabellina DSM 1414 using different sugars as carbon source.2020. Biotechprog.volum 36.issue6.
Aabid ManzoorShahaHassanMohamedabZichenZhangaYuandaSonga. Isolation, characterization and fatty acid analysis of Gilbertella persicaria DSR1: A potential new source of high value single-cell oil. 2021. Biomass and Bioenergy.Volume151.106156.
Alireza Rasouli, Seyyed Soheil Aghaee, Mohsen Zargar, Single-cell Oil Production Using Low-Cost Carbon
Sources by Newly Isolated Kocuria Y205. 2021. Archive of Hygene Science. Volume 10. Number 2.
