Genetic diversity of Tomato leaf curl Palampur virus and its whitefly vector, Bemisia tabaci, in the Sistan region
Subject Areas : Virology
javad abkhoo
1
,
Ahmad Mehraban
2
1 - Agricultural Research Institute,University of Zabol, Iran
2 - Assistant Professor, Department of Agronomy, Zahedan Branch, Islamic Azad University, Zahedan, Iran
Keywords: Sistan, phylogenetic analysis, Bemisia tabaci, Tomato leaf curl Palampur virus, Cytochrome oxidase-I gene,
Abstract :
Background & Objectives: Tomato leaf curl Palampurvirus (ToLCPMV) is one of the most important plant viruses that has been reported from some regions of Iran. In this study, we analysed the genetic diversity of Sistanian isolates of ToLCPMV and their vector, Bemisia tabaci by partial genome sequencing. Materials & Methods: Leaves of plants expressing symptoms of ToLCPMV infection and B. tabaci adults were collected from cities of the Sistan region during the autumn season of 2019. Specific primers of gene coat protein were used to detect ToLCPMV in three crops (melon, eggplant and pepper). Results: Nucleotide sequences of coat protein gene of three Sistanain isolates of ToLCPMV showed 92-99 % sequence identity with previously characterized ToLCPMV isolates. Phylogenetic analyses based on nucleotide sequences of coat protein gene indicated that in the Sistanian isolates of ToLCPMV grouped with other Iranian isolates. Eggplant and pepper represent new natural hosts of ToLCPMV in Iran. Phylogenetic analysis based on mitochondrial cytochrome oxidase-I gene sequences showed that the collected B. tabaci samples from the Sistan region and other Iranian B. tabaci samples belong to the B biotype. Bemisia tabaci samplesof Zabol, Zahak and Helmand counties showed respectively 100, 97.75 and 99.68% nucleotide sequence identity to some from Fars province samples of B. tabaci. Conclusion: In this study, the Sistanain isolates of ToLCPMV were grouped with Iranian isolates of ToLCPMV and the collected B. tabaci samples from the Sistan region belong to the B biotype.
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2. umar Y, Hallan V, Zaidi, AA. Chilli leaf curl Palampur virus is a distinct begomovirus
species associated with a betasatellite. Plant Pathol. 2011; 60:1040–1047.
3. Namrata J, Saritha R , Datta D, Singh M, Dubey RS, Rai AB, Rai M. Molecular
characterization of Tomato leaf curl Palampur virus and Pepper leaf curl betasatellite
naturally infecting pumpkin (Cucurbita moschata) in India. Indian J. Virol. 2010; 21(2):
128–132.
4. Venkataravanappa V, Prasanna HC, Lakshminarayana CN. rishna Reddy M. Molecular
Detection and Characterization of Phytoplasma in Association With Begomovirus in Eggplant.
Acta Virol. 2018; 62(3): 246-258.
5. umari S, rishnan N, Dubey V, umar Pandey , Singh J. Characterization of recombinant
tomato leaf curl Palampur virus causing leaf curl disease of Basella alba L. in India. JPP.
2020; 102: 523–527.
6. Raj S , umar S, Srivastava A. Association of tomato leaf curl Palampur virus with yellow
mosaic disease of Armenian cucumber ( Cucumis melo var. fle uoses) and wild melon
(C. callosus var. agrestis) in India. Arch Phytopath Plant Protect. 2016; 48(9):1-9.
7. Ali I, Malik AH, Mansoor S. First Report of Tomato leaf curl Palampur virus on Bitter ourd
in Pakistan. Plant Dis. 2010; 94(2): 276-276.
8. Malik AH, Briddon RW, Mansoor S. Infectious clones of Tomato leaf curl Palampur virus
with a defective DNA B and their pseudo-recombination with Tomato leaf curl New Delhi
virus. Virol. J. 2011; 8(1): 173.
9 . Heydarnejad J, Mozaffari A, Massumi H, Fazeli R , ray AJ, Meredith S,
Lak ay F. Shepherd DN , Martin DP. Varsani A. Complete sequences
of tomato leaf curl Palampur virus isolates infecting cucurbits in Iran. Arch Virol. 2009; 154
(6): 1015-8.
10. Heydarnejad J, Hesari M, Massumi H, Varsani A. Incidence and natural hosts of Tomato leaf
curl Palampur virus in Iran. Australasian Plant Pathol. 2013; 42: 195–203.
11. Jones DR. Plant viruses transmitted by whiteflies. Eur J Plant Pathol. 2003; 109: 195–219.
12. Lee W, Park J, Lee S, Lee S. Akimoto S. Taxonomic status of the Bemisia tabaci complex
(Hemiptera: Aleyrodidae) and reassessment of the number of its constituent species. PLoS
One. 2013; 8(5): e63817.
13. Rajaei Shoorcheh H, azemi B, Manzari S, Brown J . Sarafrazi A. enetic variation and
mtCO phylogeny for Bemisia tabaci (Hemiptera, Aleyrodidae) indicate that the ‘B’ biotype
predominates in Iran. J Pest Sci. 2008; 81: 199-206.
14. Shahbazi M, Behjatnia SAA, Alichi M, Roumi V. Izadpanah . Biotypes of Bemisia tabaci
( enn.) from Fars province. Iran J Plant Protect Sci; 2015; 45(2): 201-211.
15. Mulenga RM, Legg JP, Ndunguru J, Chikoti CC, Miano DW, Mutitu WE, Alabi OJ. Survey,
Molecular Detection, and Characterization of eminiviruses Associated with Cassava Mosaic
Disease in Zambia. Plant Dis. 2016; 100:1379-1387.
16. Ebadi N, Najaipour , Faghihi MM, Ayazpou . 2020. Determination of distr ibution pattern
of Candidatus Phytoplasma aurantifolia in tomato plant a evealed by Real-time PCR. JMW, 12
(4): 377-392. [In Persian]
17. Tamura , Peterson D, Peterson, N, Stecher, , Nei M. umar, S. ME A5: Molecular
evolutionary genetics analysis using maximum likelihood, evolutionary distance, and
maximum parsimony methods. Mol Biol Evol. 2011; 28: 2731-2739.
18. Frohlich DR, Torres-Jerez I, Bedford ID, Markham P , Brown J . A phylogeographical
analysis of the Bemisia tabaci species complex based on mitochondrial DNA markers. Mol
Ecol. 1999; 8: 1683-1691.
19. Dinsdale, A., Cook, L., Riginosi, C., Buckley, Y. M. De Barro, P. J. Refined global analysis
of Bemisia tabaci (Hemiptera: Sternorrhyncha: Aleyrodoidea: Aleyrodidae) mitochondrial
cytochrome oxidase 1 to identify species level genetic boundaries. Entomol Soc Am. 2010;
103: 196-208.
20. Ahmed MZ ,Yuan Sh, uiHua J, Shun Xiang R, Yu Zhou D, BaoLi . Population and host
plant differentiation of the sweetpotato whitefly, Bemisia tabaci (Homoptera: Aleyrodidae), in
East, South and Southwest China. Acta Entomologica Sinica. 2009; 52(10): 1132-1138.
21. Tahiri A, Halkett F, ranier, M, ueguen . Peterschmitt M. Evidence of gene flow between
sympatric populations of the Middle East-Asia Minor 1 and Mediterranean putative species of
Bemisia tabaci. Ecol Evol. 2013; 3: 2619–2633.
22. irk AA, Lacey LA, Brown J , Ciomperlik MA, oolsby JA, Vacek DC, Wendel LE,
Napompeth B. Variability within the Bemisia tabaci complex (Homoptera: Aleyrodidae) and
its natural enemies: successful biological control of type B in the USA. Bull Entomol Res.
2000; 90(04): 317-327.
23. Papayiannis LC, Brown J , Seraphides NA, Hadjistylli M, Ioannou N, atis NI. A Real-Time
PCR Assay to Differentiate the B and Biotypes of the Bemisia Tabaci Complex in Cyprus.
Bull Entomol Res. 2009; 99(6): 573-82.
24. Barboza Na M, Esker P, Inoue-Nagata A . Moriones E. enetic diversity and geographic
distribution of Bemisia tabaci and Trialeurodes vaporariorum in Costa Rica. Ann Appl Biol,
2019; 174(2): 1–14.
24. Shadmany M, Boykin LM, Muhamad R, Omar D. enetic Diversity of Bemisia tabaci
(Hemiptera: Aleyrodidae) Species Complex Across Malaysia. J Econ Entomol. 2019; 112 (1):
75–84.
26. Tang XT, Cai L, Shen Y, Yu-Zhou Du YZ. Diversity and evolution of the endosymbionts
of Bemisia tabaci in China. Peer J. 2018; 6: e5516.
27. areem AA, Logan SA, Port , Wolff . Bemisia tabaci in Iraq: Population structure,
endosymbiont diversity and putative species. J Appl Entomol. 2020; 144(4): 297-307.
28. umar Y. 2011. enome Organization and Mechanism of RNA Silencing Suppression of
Begomoviruses Infecting Some Solanaceous Crops. Ph. D. Institute of Himalayan Bioresource
Technology
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species infecting tomato in India. Virus enes 2008; 37:425–431.
2. umar Y, Hallan V, Zaidi, AA. Chilli leaf curl Palampur virus is a distinct begomovirus
species associated with a betasatellite. Plant Pathol. 2011; 60:1040–1047.
3. Namrata J, Saritha R , Datta D, Singh M, Dubey RS, Rai AB, Rai M. Molecular
characterization of Tomato leaf curl Palampur virus and Pepper leaf curl betasatellite
naturally infecting pumpkin (Cucurbita moschata) in India. Indian J. Virol. 2010; 21(2):
128–132.
4. Venkataravanappa V, Prasanna HC, Lakshminarayana CN. rishna Reddy M. Molecular
Detection and Characterization of Phytoplasma in Association With Begomovirus in Eggplant.
Acta Virol. 2018; 62(3): 246-258.
5. umari S, rishnan N, Dubey V, umar Pandey , Singh J. Characterization of recombinant
tomato leaf curl Palampur virus causing leaf curl disease of Basella alba L. in India. JPP.
2020; 102: 523–527.
6. Raj S , umar S, Srivastava A. Association of tomato leaf curl Palampur virus with yellow
mosaic disease of Armenian cucumber ( Cucumis melo var. fle uoses) and wild melon
(C. callosus var. agrestis) in India. Arch Phytopath Plant Protect. 2016; 48(9):1-9.
7. Ali I, Malik AH, Mansoor S. First Report of Tomato leaf curl Palampur virus on Bitter ourd
in Pakistan. Plant Dis. 2010; 94(2): 276-276.
8. Malik AH, Briddon RW, Mansoor S. Infectious clones of Tomato leaf curl Palampur virus
with a defective DNA B and their pseudo-recombination with Tomato leaf curl New Delhi
virus. Virol. J. 2011; 8(1): 173.
9 . Heydarnejad J, Mozaffari A, Massumi H, Fazeli R , ray AJ, Meredith S,
Lak ay F. Shepherd DN , Martin DP. Varsani A. Complete sequences
of tomato leaf curl Palampur virus isolates infecting cucurbits in Iran. Arch Virol. 2009; 154
(6): 1015-8.
10. Heydarnejad J, Hesari M, Massumi H, Varsani A. Incidence and natural hosts of Tomato leaf
curl Palampur virus in Iran. Australasian Plant Pathol. 2013; 42: 195–203.
11. Jones DR. Plant viruses transmitted by whiteflies. Eur J Plant Pathol. 2003; 109: 195–219.
12. Lee W, Park J, Lee S, Lee S. Akimoto S. Taxonomic status of the Bemisia tabaci complex
(Hemiptera: Aleyrodidae) and reassessment of the number of its constituent species. PLoS
One. 2013; 8(5): e63817.
13. Rajaei Shoorcheh H, azemi B, Manzari S, Brown J . Sarafrazi A. enetic variation and
mtCO phylogeny for Bemisia tabaci (Hemiptera, Aleyrodidae) indicate that the ‘B’ biotype
predominates in Iran. J Pest Sci. 2008; 81: 199-206.
14. Shahbazi M, Behjatnia SAA, Alichi M, Roumi V. Izadpanah . Biotypes of Bemisia tabaci
( enn.) from Fars province. Iran J Plant Protect Sci; 2015; 45(2): 201-211.
15. Mulenga RM, Legg JP, Ndunguru J, Chikoti CC, Miano DW, Mutitu WE, Alabi OJ. Survey,
Molecular Detection, and Characterization of eminiviruses Associated with Cassava Mosaic
Disease in Zambia. Plant Dis. 2016; 100:1379-1387.
16. Ebadi N, Najaipour , Faghihi MM, Ayazpou . 2020. Determination of distr ibution pattern
of Candidatus Phytoplasma aurantifolia in tomato plant a evealed by Real-time PCR. JMW, 12
(4): 377-392. [In Persian]
17. Tamura , Peterson D, Peterson, N, Stecher, , Nei M. umar, S. ME A5: Molecular
evolutionary genetics analysis using maximum likelihood, evolutionary distance, and
maximum parsimony methods. Mol Biol Evol. 2011; 28: 2731-2739.
18. Frohlich DR, Torres-Jerez I, Bedford ID, Markham P , Brown J . A phylogeographical
analysis of the Bemisia tabaci species complex based on mitochondrial DNA markers. Mol
Ecol. 1999; 8: 1683-1691.
19. Dinsdale, A., Cook, L., Riginosi, C., Buckley, Y. M. De Barro, P. J. Refined global analysis
of Bemisia tabaci (Hemiptera: Sternorrhyncha: Aleyrodoidea: Aleyrodidae) mitochondrial
cytochrome oxidase 1 to identify species level genetic boundaries. Entomol Soc Am. 2010;
103: 196-208.
20. Ahmed MZ ,Yuan Sh, uiHua J, Shun Xiang R, Yu Zhou D, BaoLi . Population and host
plant differentiation of the sweetpotato whitefly, Bemisia tabaci (Homoptera: Aleyrodidae), in
East, South and Southwest China. Acta Entomologica Sinica. 2009; 52(10): 1132-1138.
21. Tahiri A, Halkett F, ranier, M, ueguen . Peterschmitt M. Evidence of gene flow between
sympatric populations of the Middle East-Asia Minor 1 and Mediterranean putative species of
Bemisia tabaci. Ecol Evol. 2013; 3: 2619–2633.
22. irk AA, Lacey LA, Brown J , Ciomperlik MA, oolsby JA, Vacek DC, Wendel LE,
Napompeth B. Variability within the Bemisia tabaci complex (Homoptera: Aleyrodidae) and
its natural enemies: successful biological control of type B in the USA. Bull Entomol Res.
2000; 90(04): 317-327.
23. Papayiannis LC, Brown J , Seraphides NA, Hadjistylli M, Ioannou N, atis NI. A Real-Time
PCR Assay to Differentiate the B and Biotypes of the Bemisia Tabaci Complex in Cyprus.
Bull Entomol Res. 2009; 99(6): 573-82.
24. Barboza Na M, Esker P, Inoue-Nagata A . Moriones E. enetic diversity and geographic
distribution of Bemisia tabaci and Trialeurodes vaporariorum in Costa Rica. Ann Appl Biol,
2019; 174(2): 1–14.
24. Shadmany M, Boykin LM, Muhamad R, Omar D. enetic Diversity of Bemisia tabaci
(Hemiptera: Aleyrodidae) Species Complex Across Malaysia. J Econ Entomol. 2019; 112 (1):
75–84.
26. Tang XT, Cai L, Shen Y, Yu-Zhou Du YZ. Diversity and evolution of the endosymbionts
of Bemisia tabaci in China. Peer J. 2018; 6: e5516.
27. areem AA, Logan SA, Port , Wolff . Bemisia tabaci in Iraq: Population structure,
endosymbiont diversity and putative species. J Appl Entomol. 2020; 144(4): 297-307.
28. umar Y. 2011. enome Organization and Mechanism of RNA Silencing Suppression of
Begomoviruses Infecting Some Solanaceous Crops. Ph. D. Institute of Himalayan Bioresource
Technology
