The production and antibacterial effectiveness of silver nanocrystalline dressing
Subject Areas : Microbial BiotechnologyMarjan Enshaeieh 1 , Azadeh Abdoli 2 , Reza Montazeri 3
1 - MS.c., Young Researchers and Elite Club, Falavarjan branch, Islamic Azad University, Esfahan, Iran
2 - MS.c., Young Researchers and Elite Club, Falavarjan branch, Islamic Azad University, Esfahan, Iran.
3 - BS.c., Department of Biology, Science branch, Esfahan University, Esfahan, Iran.
Keywords: Staphylococcus aureus, Escherichia coli, Taguchi method, Silver dressing,
Abstract :
Background & Objectives: Since the wound infections caused by gram positive and negative bacteria are very common, application of Nanocrystalline silver in wound healing bands is recently of one of points of research interests. The epithelization effect and control of wound infection are the most important factors for selection of disinfecting factors in these bands. This study was performed to produce nanocrystalline silver bands and to investigate their efficiency on control of selected gram positive and negative bacteria. Materials & Methods: In this study, silver nanocrystalline dressing was produced using nylon dressing and silver ions. Thereafter, we evaluate its antibacterial effect against Staphylococcus aureus and Escherichia coli. The experiments were designed based on Taguchi method for investigation different parameters on antimicrobial effect of silver dressing. Results: The silver dressing showed an impressive effect on inhibition of the growth of mentioned bacteria and leads to the inhibition zone diameter of 5.5 cm around the dressing pieces. AgNO3 was the most important parameter in determination of the antimicrobial effect of silver dressing (approximately 65% efficiency). Conclusion: Silver nanocrystalline dressing was effective on inhibition of microorganisms growth. Furthermore, application of Taguchi method was highly effective in optimization process of this investigation.
1. Lansdown ABG. Silver I: its antibacterial properties and mechanism of action. J Wound Care. 2002; 11(4): 125–130.
2. Wright JB, Lam K, Burrell RE. Wound management in an era of increasing bacterial antibiotic resistance: a role for topical silver. Am J Infect Control. 1998; 26: 572–575.
3. Vermeulen H, Ubbink DT, Storm-Versloot MN. Topical silver for treating infected wounds (Protocol). Cochrane Database Syst Rev. 2005; (1): 54- 86.
4. Warriner R, Burrell R. Infection and the chronic wound: A focus on silver. Adv Skin Wound Care. 2005; 18: 2–12.
5. Li XZ, Nikaido H, Williams KE. Silver-resistant mutants of Escherichia coli display active efflux of Ag+ and are deficient in porins. J Bacteriol. 1997; 179(19): 6127–6132.
6. Dowsett C. An overview of Acticoat dressing in wound management. Br J Nurs. 2003; 12(19): 44–49.
7. Paddock HN, Schultz GS, Perrin KJ. Clinical assessment of silver-coated antimicrobial dressings on MMPs and cytokine levels in non-healing wounds. Wound Repair Regen. 2002; 10: 45- 52.
8. Wright JB, Lam K, Buret AG, Olson ME, Burrell RE. Early healing events in a porcine model of contaminated wounds: effects of nanocrystalline silver on matrix metalloproteinases, cell apoptosis, and healing. Wound Repair Regen. 2002; 10: 141–151.
9. Mooney EK, Lippitt C, Friedman J. Silver dressings [safety and efficacyreports]. Plast & Reconst Surgery J. 2006; 117(2): 666–669.
10. Khundkar R, Malic C, Burge T. Use of acticoat dressings in burns: what is the evidence? Burns. 2010; 36(6): 751–758.
11. Atiyeh BS, Costagliola M, Hayek SN, Dibo SA. Effect of silver on burn wound infection control and healing: review of the literature. Burns. 2007; 33(2): 139–148.
12. Liedberg H, Lundeberg T. Assessment of silver-coated urinary catheter toxicity by cell culture. Urological Res. 1989; 17 (6): 359–362.
13. Hoffman S. Silver sulfadiazine: an antibacterial agent for topical use in burns. Scand J Plast Reconstr Surgery. 1984; 18: 119-124.
14. Klasen H. A historical review of the use of silver in the treatment of burns. II. Renewed interest for silver. Burns. 2000; 26: 131-136.
15. Demling R, DeSanti L. The role of silver technology in wound healing: Part 1: Effects of silver on wound management. Wounds. 2001; 13: 131-146.
16. Lansdown A. Silver 1: its antibacterial properties and mechanism of action. J Wound Care. 2002; 11:125-128.
17. Dunn K, Edwards-Jones V. The role of Acticoat™ with nanocrystalline silver in the management of burns. Burns. 2000; 1: 1-9.
18. Orvington L. The truth about silver. Ostomy Wound Manage. 2004; 50: 1-10.
19. Fong J. The use of silver products in the management of burn wounds: change in practice for the burn unit at Royal Perth Hospital. Primary Intention. 2005; 13: 16-28.
20. Ang E. Glasg F, Lee T, Edinb F, Christine S, Gan G. Pain control in a randomized, controlled, clinical trial comparing moist exposed burn ointment and conventional methods in patients with partial-thickness burns. J Burn Care Rehabil. 2003; 24(5): 289-296
21. Atiyeh BS, Dibo SA, Hayek SN. Wound cleansing, topical antiseptics and wound healing. Int Wound J. 2009; 6(6): 420-430.
22. Stepanov AL, Popok VN, Hole DE. Glass physics and chemistry. Russian Aca of Sci & Inst of Silicate Chem. 2002; 28(2): 90-98.
23. Dessy YS, Cheng-Kang L. Magnetic antimicrobial nanocomposite based on bacterial cellulose and silver nanoparticles Manthiriyappan Sureshkumar. J Mater Chem. 2010; 20: 6948-6955.
24. Montazer M, Alimohammadi F, Shamei A, Rahimi MK. In situ synthesis of nano silver on cotton using Tollens’ reagent. Carbohydrate Polymers. 2012; 87: 1706-1712.
25. Jung WK, Koo HC, Kim KW, shin S, Kim SH, Park YH. Antibacterial activity and mechanism of the silver ion in Staphylococcus aureus and Escherchia coli. Appl Environ Microbiol. 2008; 74: 2171-2178.
26. Sondi I, Salopek-Sondi B. Silver nanoparticles as antimicrobial agent: a case study on E. coli as a model for Gram-negative bacteria. J Colloid Interface Sci. 2004; 275: 177-182.
27. Stoimenov PK, Klinger RL, Marchin GL, Klabunde KJ. Metal oxide nanoparticles as bactericidal agents. Langmuir. American Chem Soc. 2002; 18(17): 6679-6686.
28. Kędziora A, Gorzelańczyk K, Bugla-Płoskońska G. Positive and negative aspects of silver nanoparticles usage. Biol Int. 2012; 53: 68-76.
29. Sadeghi B, Gamaroudi FS, Hashemi M, Nezhad HR, Nasrollahi M, Ardalan S. Comparison of the anti-bacterial activity on the nanosilver shapes: nanoparticles, nanorods and nanoplates. Advanced Powder Technol. 2012; 23(1): 22–26.
30. Radzig MA, Nadtochenko VA, Koksharova OA, Kiwi J, Lipasova VA, Khmel IA. Antibacterial effects of silver nanoparticles on gram-negative bacteria: Influence on the growth and biofilms formation, mechanisms of action. Colloids and Surfaces B: Biointerfaces. 2013; 102: 300-306.
31. Lara H, Garza-Treviño E, Ixtepan-Turrent L, Singh DK. Silver nanoparticles are broad-spectrum bactericidal and virucidal compounds. J Nanobiotech. 2011; 9: 3155-3159.
32. Rajawat S, Shums Qureshi M. Comparative study on bactericidal effect of silver nanoparticles, synthesized using green technology, in combination with antibiotics on Salmonella typhi. J Biomaterials Nanobiotech. 2012; 3: 480-485.
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1. Lansdown ABG. Silver I: its antibacterial properties and mechanism of action. J Wound Care. 2002; 11(4): 125–130.
2. Wright JB, Lam K, Burrell RE. Wound management in an era of increasing bacterial antibiotic resistance: a role for topical silver. Am J Infect Control. 1998; 26: 572–575.
3. Vermeulen H, Ubbink DT, Storm-Versloot MN. Topical silver for treating infected wounds (Protocol). Cochrane Database Syst Rev. 2005; (1): 54- 86.
4. Warriner R, Burrell R. Infection and the chronic wound: A focus on silver. Adv Skin Wound Care. 2005; 18: 2–12.
5. Li XZ, Nikaido H, Williams KE. Silver-resistant mutants of Escherichia coli display active efflux of Ag+ and are deficient in porins. J Bacteriol. 1997; 179(19): 6127–6132.
6. Dowsett C. An overview of Acticoat dressing in wound management. Br J Nurs. 2003; 12(19): 44–49.
7. Paddock HN, Schultz GS, Perrin KJ. Clinical assessment of silver-coated antimicrobial dressings on MMPs and cytokine levels in non-healing wounds. Wound Repair Regen. 2002; 10: 45- 52.
8. Wright JB, Lam K, Buret AG, Olson ME, Burrell RE. Early healing events in a porcine model of contaminated wounds: effects of nanocrystalline silver on matrix metalloproteinases, cell apoptosis, and healing. Wound Repair Regen. 2002; 10: 141–151.
9. Mooney EK, Lippitt C, Friedman J. Silver dressings [safety and efficacyreports]. Plast & Reconst Surgery J. 2006; 117(2): 666–669.
10. Khundkar R, Malic C, Burge T. Use of acticoat dressings in burns: what is the evidence? Burns. 2010; 36(6): 751–758.
11. Atiyeh BS, Costagliola M, Hayek SN, Dibo SA. Effect of silver on burn wound infection control and healing: review of the literature. Burns. 2007; 33(2): 139–148.
12. Liedberg H, Lundeberg T. Assessment of silver-coated urinary catheter toxicity by cell culture. Urological Res. 1989; 17 (6): 359–362.
13. Hoffman S. Silver sulfadiazine: an antibacterial agent for topical use in burns. Scand J Plast Reconstr Surgery. 1984; 18: 119-124.
14. Klasen H. A historical review of the use of silver in the treatment of burns. II. Renewed interest for silver. Burns. 2000; 26: 131-136.
15. Demling R, DeSanti L. The role of silver technology in wound healing: Part 1: Effects of silver on wound management. Wounds. 2001; 13: 131-146.
16. Lansdown A. Silver 1: its antibacterial properties and mechanism of action. J Wound Care. 2002; 11:125-128.
17. Dunn K, Edwards-Jones V. The role of Acticoat™ with nanocrystalline silver in the management of burns. Burns. 2000; 1: 1-9.
18. Orvington L. The truth about silver. Ostomy Wound Manage. 2004; 50: 1-10.
19. Fong J. The use of silver products in the management of burn wounds: change in practice for the burn unit at Royal Perth Hospital. Primary Intention. 2005; 13: 16-28.
20. Ang E. Glasg F, Lee T, Edinb F, Christine S, Gan G. Pain control in a randomized, controlled, clinical trial comparing moist exposed burn ointment and conventional methods in patients with partial-thickness burns. J Burn Care Rehabil. 2003; 24(5): 289-296
21. Atiyeh BS, Dibo SA, Hayek SN. Wound cleansing, topical antiseptics and wound healing. Int Wound J. 2009; 6(6): 420-430.
22. Stepanov AL, Popok VN, Hole DE. Glass physics and chemistry. Russian Aca of Sci & Inst of Silicate Chem. 2002; 28(2): 90-98.
23. Dessy YS, Cheng-Kang L. Magnetic antimicrobial nanocomposite based on bacterial cellulose and silver nanoparticles Manthiriyappan Sureshkumar. J Mater Chem. 2010; 20: 6948-6955.
24. Montazer M, Alimohammadi F, Shamei A, Rahimi MK. In situ synthesis of nano silver on cotton using Tollens’ reagent. Carbohydrate Polymers. 2012; 87: 1706-1712.
25. Jung WK, Koo HC, Kim KW, shin S, Kim SH, Park YH. Antibacterial activity and mechanism of the silver ion in Staphylococcus aureus and Escherchia coli. Appl Environ Microbiol. 2008; 74: 2171-2178.
26. Sondi I, Salopek-Sondi B. Silver nanoparticles as antimicrobial agent: a case study on E. coli as a model for Gram-negative bacteria. J Colloid Interface Sci. 2004; 275: 177-182.
27. Stoimenov PK, Klinger RL, Marchin GL, Klabunde KJ. Metal oxide nanoparticles as bactericidal agents. Langmuir. American Chem Soc. 2002; 18(17): 6679-6686.
28. Kędziora A, Gorzelańczyk K, Bugla-Płoskońska G. Positive and negative aspects of silver nanoparticles usage. Biol Int. 2012; 53: 68-76.
29. Sadeghi B, Gamaroudi FS, Hashemi M, Nezhad HR, Nasrollahi M, Ardalan S. Comparison of the anti-bacterial activity on the nanosilver shapes: nanoparticles, nanorods and nanoplates. Advanced Powder Technol. 2012; 23(1): 22–26.
30. Radzig MA, Nadtochenko VA, Koksharova OA, Kiwi J, Lipasova VA, Khmel IA. Antibacterial effects of silver nanoparticles on gram-negative bacteria: Influence on the growth and biofilms formation, mechanisms of action. Colloids and Surfaces B: Biointerfaces. 2013; 102: 300-306.
31. Lara H, Garza-Treviño E, Ixtepan-Turrent L, Singh DK. Silver nanoparticles are broad-spectrum bactericidal and virucidal compounds. J Nanobiotech. 2011; 9: 3155-3159.
32. Rajawat S, Shums Qureshi M. Comparative study on bactericidal effect of silver nanoparticles, synthesized using green technology, in combination with antibiotics on Salmonella typhi. J Biomaterials Nanobiotech. 2012; 3: 480-485.
