تولید و اثر بخشی ضدباکتریایی پانسمان های نانوکریستال نقره
الموضوعات :مرجان انشاییه 1 , آزاده عبدلی 2 , رضا منتظری 3
1 - کارشناس ارشد، دانشگاه آزاد اسلامی، واحد فلاورجان، باشگاه پژوهشگران جوان و نخبگان، اصفهان
2 - کارشناس ارشد، دانشگاه آزاد اسلامی، واحد فلاورجان، باشگاه پژوهشگران جوان و نخبگان، اصفهان
3 - کارشناس، دانشکده علوم، گروه زیست شناسی، دانشگاه اصفهان
الکلمات المفتاحية: اشریشیا کلی, استافیلوکوکوس اورئوس, پانسمان نقره, روش تاگوچی,
ملخص المقالة :
سابقه و هدف: در سال های اخیر پانسمان های نقره به دلیل تاثیر بر روی باکتری های گرم منفی و مثبت مورد توجه زیادی قرارگرفته اند. عوامل موثر در انتخاب پانسمان ها شامل میزان تاثیر ضد عفونی کنندگی، میزان اثر آن بر اپیتلاسیون و توانایی آن در کنترل عفونت زخم می باشد. این مطالعه با هدف ارزیابی تولید و اثر بخشی پانسمان های نانوکریستال نقره بر کنترل رشد برخی از باکتری های گرم مثبت و گرم منفی انجام شد. مواد و روش ها: در این مطالعه با استفاده از پارچه های نایلونی و یون نقره، پانسمان نانو کریستال نقره تولید شد. سپس اثر ضد میکروبی آن بر روی استافیلوکوکوس اورئوس و اشریشیا کلی بررسی گردید. از طراحی آزمون تاگوچی به منظور بررسی میزان تاثیر عوامل مختلف بر قدرت ضد میکروبی پانسمان نقره استفاده شد. یافته ها: پانسمان نقره اثر بسیار خوبی بر روی عدم رشد باکتری های یاد شده داشت. به طوری که موجب ایجاد هاله عدم رشد به قطر میانگین 5.5 سانتی متر در اطراف قطعات پانسمان شد. مهمترین عامل در شدت قدرت ضد میکروبی پانسمان نقره میزان نیترات نقره مورد استفاده با تاثیر65 درصد بود. نتیجه گیری: پانسمان های نانوکریستال نقره نقش موثری در مهار رشد باکتری های مورد پژوهش داشت. همچنین استفاده از آزمون تاگوچی نقش موثری در بهینه سازی اثر بخشی تاثیر نانوذرات ایفا نمود.
1. Lansdown ABG. Silver I: its antibacterial properties and mechanism of action. J Wound Care. 2002; 11(4): 125–130.
2. Wright JB, Lam K, Burrell RE. Wound management in an era of increasing bacterial antibiotic resistance: a role for topical silver. Am J Infect Control. 1998; 26: 572–575.
3. Vermeulen H, Ubbink DT, Storm-Versloot MN. Topical silver for treating infected wounds (Protocol). Cochrane Database Syst Rev. 2005; (1): 54- 86.
4. Warriner R, Burrell R. Infection and the chronic wound: A focus on silver. Adv Skin Wound Care. 2005; 18: 2–12.
5. Li XZ, Nikaido H, Williams KE. Silver-resistant mutants of Escherichia coli display active efflux of Ag+ and are deficient in porins. J Bacteriol. 1997; 179(19): 6127–6132.
6. Dowsett C. An overview of Acticoat dressing in wound management. Br J Nurs. 2003; 12(19): 44–49.
7. Paddock HN, Schultz GS, Perrin KJ. Clinical assessment of silver-coated antimicrobial dressings on MMPs and cytokine levels in non-healing wounds. Wound Repair Regen. 2002; 10: 45- 52.
8. Wright JB, Lam K, Buret AG, Olson ME, Burrell RE. Early healing events in a porcine model of contaminated wounds: effects of nanocrystalline silver on matrix metalloproteinases, cell apoptosis, and healing. Wound Repair Regen. 2002; 10: 141–151.
9. Mooney EK, Lippitt C, Friedman J. Silver dressings [safety and efficacyreports]. Plast & Reconst Surgery J. 2006; 117(2): 666–669.
10. Khundkar R, Malic C, Burge T. Use of acticoat dressings in burns: what is the evidence? Burns. 2010; 36(6): 751–758.
11. Atiyeh BS, Costagliola M, Hayek SN, Dibo SA. Effect of silver on burn wound infection control and healing: review of the literature. Burns. 2007; 33(2): 139–148.
12. Liedberg H, Lundeberg T. Assessment of silver-coated urinary catheter toxicity by cell culture. Urological Res. 1989; 17 (6): 359–362.
13. Hoffman S. Silver sulfadiazine: an antibacterial agent for topical use in burns. Scand J Plast Reconstr Surgery. 1984; 18: 119-124.
14. Klasen H. A historical review of the use of silver in the treatment of burns. II. Renewed interest for silver. Burns. 2000; 26: 131-136.
15. Demling R, DeSanti L. The role of silver technology in wound healing: Part 1: Effects of silver on wound management. Wounds. 2001; 13: 131-146.
16. Lansdown A. Silver 1: its antibacterial properties and mechanism of action. J Wound Care. 2002; 11:125-128.
17. Dunn K, Edwards-Jones V. The role of Acticoat™ with nanocrystalline silver in the management of burns. Burns. 2000; 1: 1-9.
18. Orvington L. The truth about silver. Ostomy Wound Manage. 2004; 50: 1-10.
19. Fong J. The use of silver products in the management of burn wounds: change in practice for the burn unit at Royal Perth Hospital. Primary Intention. 2005; 13: 16-28.
20. Ang E. Glasg F, Lee T, Edinb F, Christine S, Gan G. Pain control in a randomized, controlled, clinical trial comparing moist exposed burn ointment and conventional methods in patients with partial-thickness burns. J Burn Care Rehabil. 2003; 24(5): 289-296
21. Atiyeh BS, Dibo SA, Hayek SN. Wound cleansing, topical antiseptics and wound healing. Int Wound J. 2009; 6(6): 420-430.
22. Stepanov AL, Popok VN, Hole DE. Glass physics and chemistry. Russian Aca of Sci & Inst of Silicate Chem. 2002; 28(2): 90-98.
23. Dessy YS, Cheng-Kang L. Magnetic antimicrobial nanocomposite based on bacterial cellulose and silver nanoparticles Manthiriyappan Sureshkumar. J Mater Chem. 2010; 20: 6948-6955.
24. Montazer M, Alimohammadi F, Shamei A, Rahimi MK. In situ synthesis of nano silver on cotton using Tollens’ reagent. Carbohydrate Polymers. 2012; 87: 1706-1712.
25. Jung WK, Koo HC, Kim KW, shin S, Kim SH, Park YH. Antibacterial activity and mechanism of the silver ion in Staphylococcus aureus and Escherchia coli. Appl Environ Microbiol. 2008; 74: 2171-2178.
26. Sondi I, Salopek-Sondi B. Silver nanoparticles as antimicrobial agent: a case study on E. coli as a model for Gram-negative bacteria. J Colloid Interface Sci. 2004; 275: 177-182.
27. Stoimenov PK, Klinger RL, Marchin GL, Klabunde KJ. Metal oxide nanoparticles as bactericidal agents. Langmuir. American Chem Soc. 2002; 18(17): 6679-6686.
28. Kędziora A, Gorzelańczyk K, Bugla-Płoskońska G. Positive and negative aspects of silver nanoparticles usage. Biol Int. 2012; 53: 68-76.
29. Sadeghi B, Gamaroudi FS, Hashemi M, Nezhad HR, Nasrollahi M, Ardalan S. Comparison of the anti-bacterial activity on the nanosilver shapes: nanoparticles, nanorods and nanoplates. Advanced Powder Technol. 2012; 23(1): 22–26.
30. Radzig MA, Nadtochenko VA, Koksharova OA, Kiwi J, Lipasova VA, Khmel IA. Antibacterial effects of silver nanoparticles on gram-negative bacteria: Influence on the growth and biofilms formation, mechanisms of action. Colloids and Surfaces B: Biointerfaces. 2013; 102: 300-306.
31. Lara H, Garza-Treviño E, Ixtepan-Turrent L, Singh DK. Silver nanoparticles are broad-spectrum bactericidal and virucidal compounds. J Nanobiotech. 2011; 9: 3155-3159.
32. Rajawat S, Shums Qureshi M. Comparative study on bactericidal effect of silver nanoparticles, synthesized using green technology, in combination with antibiotics on Salmonella typhi. J Biomaterials Nanobiotech. 2012; 3: 480-485.
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1. Lansdown ABG. Silver I: its antibacterial properties and mechanism of action. J Wound Care. 2002; 11(4): 125–130.
2. Wright JB, Lam K, Burrell RE. Wound management in an era of increasing bacterial antibiotic resistance: a role for topical silver. Am J Infect Control. 1998; 26: 572–575.
3. Vermeulen H, Ubbink DT, Storm-Versloot MN. Topical silver for treating infected wounds (Protocol). Cochrane Database Syst Rev. 2005; (1): 54- 86.
4. Warriner R, Burrell R. Infection and the chronic wound: A focus on silver. Adv Skin Wound Care. 2005; 18: 2–12.
5. Li XZ, Nikaido H, Williams KE. Silver-resistant mutants of Escherichia coli display active efflux of Ag+ and are deficient in porins. J Bacteriol. 1997; 179(19): 6127–6132.
6. Dowsett C. An overview of Acticoat dressing in wound management. Br J Nurs. 2003; 12(19): 44–49.
7. Paddock HN, Schultz GS, Perrin KJ. Clinical assessment of silver-coated antimicrobial dressings on MMPs and cytokine levels in non-healing wounds. Wound Repair Regen. 2002; 10: 45- 52.
8. Wright JB, Lam K, Buret AG, Olson ME, Burrell RE. Early healing events in a porcine model of contaminated wounds: effects of nanocrystalline silver on matrix metalloproteinases, cell apoptosis, and healing. Wound Repair Regen. 2002; 10: 141–151.
9. Mooney EK, Lippitt C, Friedman J. Silver dressings [safety and efficacyreports]. Plast & Reconst Surgery J. 2006; 117(2): 666–669.
10. Khundkar R, Malic C, Burge T. Use of acticoat dressings in burns: what is the evidence? Burns. 2010; 36(6): 751–758.
11. Atiyeh BS, Costagliola M, Hayek SN, Dibo SA. Effect of silver on burn wound infection control and healing: review of the literature. Burns. 2007; 33(2): 139–148.
12. Liedberg H, Lundeberg T. Assessment of silver-coated urinary catheter toxicity by cell culture. Urological Res. 1989; 17 (6): 359–362.
13. Hoffman S. Silver sulfadiazine: an antibacterial agent for topical use in burns. Scand J Plast Reconstr Surgery. 1984; 18: 119-124.
14. Klasen H. A historical review of the use of silver in the treatment of burns. II. Renewed interest for silver. Burns. 2000; 26: 131-136.
15. Demling R, DeSanti L. The role of silver technology in wound healing: Part 1: Effects of silver on wound management. Wounds. 2001; 13: 131-146.
16. Lansdown A. Silver 1: its antibacterial properties and mechanism of action. J Wound Care. 2002; 11:125-128.
17. Dunn K, Edwards-Jones V. The role of Acticoat™ with nanocrystalline silver in the management of burns. Burns. 2000; 1: 1-9.
18. Orvington L. The truth about silver. Ostomy Wound Manage. 2004; 50: 1-10.
19. Fong J. The use of silver products in the management of burn wounds: change in practice for the burn unit at Royal Perth Hospital. Primary Intention. 2005; 13: 16-28.
20. Ang E. Glasg F, Lee T, Edinb F, Christine S, Gan G. Pain control in a randomized, controlled, clinical trial comparing moist exposed burn ointment and conventional methods in patients with partial-thickness burns. J Burn Care Rehabil. 2003; 24(5): 289-296
21. Atiyeh BS, Dibo SA, Hayek SN. Wound cleansing, topical antiseptics and wound healing. Int Wound J. 2009; 6(6): 420-430.
22. Stepanov AL, Popok VN, Hole DE. Glass physics and chemistry. Russian Aca of Sci & Inst of Silicate Chem. 2002; 28(2): 90-98.
23. Dessy YS, Cheng-Kang L. Magnetic antimicrobial nanocomposite based on bacterial cellulose and silver nanoparticles Manthiriyappan Sureshkumar. J Mater Chem. 2010; 20: 6948-6955.
24. Montazer M, Alimohammadi F, Shamei A, Rahimi MK. In situ synthesis of nano silver on cotton using Tollens’ reagent. Carbohydrate Polymers. 2012; 87: 1706-1712.
25. Jung WK, Koo HC, Kim KW, shin S, Kim SH, Park YH. Antibacterial activity and mechanism of the silver ion in Staphylococcus aureus and Escherchia coli. Appl Environ Microbiol. 2008; 74: 2171-2178.
26. Sondi I, Salopek-Sondi B. Silver nanoparticles as antimicrobial agent: a case study on E. coli as a model for Gram-negative bacteria. J Colloid Interface Sci. 2004; 275: 177-182.
27. Stoimenov PK, Klinger RL, Marchin GL, Klabunde KJ. Metal oxide nanoparticles as bactericidal agents. Langmuir. American Chem Soc. 2002; 18(17): 6679-6686.
28. Kędziora A, Gorzelańczyk K, Bugla-Płoskońska G. Positive and negative aspects of silver nanoparticles usage. Biol Int. 2012; 53: 68-76.
29. Sadeghi B, Gamaroudi FS, Hashemi M, Nezhad HR, Nasrollahi M, Ardalan S. Comparison of the anti-bacterial activity on the nanosilver shapes: nanoparticles, nanorods and nanoplates. Advanced Powder Technol. 2012; 23(1): 22–26.
30. Radzig MA, Nadtochenko VA, Koksharova OA, Kiwi J, Lipasova VA, Khmel IA. Antibacterial effects of silver nanoparticles on gram-negative bacteria: Influence on the growth and biofilms formation, mechanisms of action. Colloids and Surfaces B: Biointerfaces. 2013; 102: 300-306.
31. Lara H, Garza-Treviño E, Ixtepan-Turrent L, Singh DK. Silver nanoparticles are broad-spectrum bactericidal and virucidal compounds. J Nanobiotech. 2011; 9: 3155-3159.
32. Rajawat S, Shums Qureshi M. Comparative study on bactericidal effect of silver nanoparticles, synthesized using green technology, in combination with antibiotics on Salmonella typhi. J Biomaterials Nanobiotech. 2012; 3: 480-485.
