Effects of Dunaliella salina on different levels of IgM, IgG, IgA and IgE immunoglobulins in Rainbow trout (Oncrohynchus mykiss)

Subject Areas : Marine Biology

P. Amaninejad ¹ (Faculty of Marine Science & Technology, Islamic Azad University, North Tehran Branch)
H. Emadi ² (Faculty of Marine Science & Technology, Islamic Azad University, North Tehran Branch)
M. Emtyazjoo ³ (Faculty of Marine Science & Technology, Islamic Azad University, North Tehran Branch)
H. Hosseinzade Sahafi ⁴ (Iranian Fisheries Research Organization)

Received: 2010-05-30 Accepted : 2012-05-04 Published : 2012-06-19

Keywords: Rainbow trout, immunoglobulin, Dunaliella salina,

Abstract :

Main purpose of this research was to study the effects of Dunaliella microalgae on immunological and survival rate of Rainbow trout. It was planned to measure IgM, IgG, IgA and IgE blood immunoglobulins and find out occurred changes in survival rate of the fish. Rainbow trout specimens were separated in five groups and were fed with diets containing 0, 5, 7, 9 and 11 grams of pure dried Dunaliella in each kilogram of food respectively. Blood samples were taken from 25 random collected fish, at the end of first and third months of culture and sent to the laboratory to measure immunoglobulin factors. Results indicated that levels of IgM and IgA immunoglobulins in the blood plasma of fish fed with Dunaliella algae showed significant statistical differences (P<0.05) with those not received Dunaliella algae. With increased levels of Dunliella in food, and increased weight, levels of IgM and IgA immunoglobulins were also increased. Their measured amounts were 10± 1 and 2 ± 1 mg per deciliter of blood plasma respectively. IgG and IgE immunoglobulins showed no significant difference. No mortality was observed in any of the groups, showing good adaptation with feeding conditions. Paying attention to the obtained results, indicate that Dunaliella algae containing β- Carotene has favorable effects on immunology, survival and growth of rainbow trout.