The effect of warfarin on the development of male rat offspring cerebellum

Subject Areas : Developmental biology of plants and animals , development and differentiation in microorganisms

Mino Mahmoodi ¹ ( Department of Biology, Faculty of Basic Sciences, Islamic Azad University,Hamadan Branch, Hamadan, Iran. )
Mona Baroodabi ² ( Department of Biology, Faculty of Basic Sciences, Islamic Azad University,Hamadan Branch, Hamadan, Iran. )
Siyamak Shahidi ³ ( Neurophysiology Research Center, Hamadan University of Medical Sciences, Hamadan, Iran. )

Received: 2016-01-10 Accepted : 2016-02-20 Published : 2016-05-21

Keywords: Embryo, Rat, Cerebellum, Warfarin,

Abstract :

Warfarin is an oral anticoagulant agent prescribed for treating cardio-vascular disorder. It reduces the synthesis of vitamin K- dependent factors. As it can easily pass through the placenta, have unwilling effects on embryo. The aim of this study was to evaluate the effect of warfarin on the brain development of rat's offspring. In this study, 30 pregnant wistar rats were randomly divided in 5 groups, control, receiving normal saline, and receiving warfarin in doses at 0.25, 0.5, 0.65 ml/kg during days 14 – 18 of gestation by gavages. 40 days after birth, 6 male newborn were randomly selected in each groups. After anesthesia, serial cross sections of the left hemisphere of the brain were prepared and histological studies were done. The data were analyzed by using one-way ANOVA and Tukey post-test. P

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