The effect of Lactobacillus casei and Bifidobacterium lactis probiotics on expression of RAGE and TGFβ genes in kidney tissue of diabetic rats
Subject Areas : Developmental biology of plants and animals , development and differentiation in microorganisms
zahra keshtmand
1
,
ghazaleh alizadeh
2
1 - , Department of Biology, Central Tehran Branch, Islamic Azad University, Tehran, Iran.
2 - Department of Biology, Central Tehran Branch, Islamic Azad University, Tehran, Iran.
Keywords: probiotics, Diabetes, Streptozotocin, RAGE, TGFβ,
Abstract :
Background and aims: Diabetic nephropathy is one of the most common complications of diabetes that increases the mortality and mortality of patients with diabetes. Probiotics, due to their function, can help control diabetes as dietary supplements.. The aim of this study was to evaluate the effects of Lactobacillus casei and Bifidobacterium lactis probiotics on the expression of RAGE and TGFβ gene in the tissues of all diabetic rats. Materials and Methods: In this experimental study, 35 rats were grouped into 5 groups (n = 7) control, diabetic, and three diabetic groups receiving Lactobacillus casei, Bifidobacterium lactis and a mixture of both probiotics. Diabetic mice received a single dose of streptozotocin intraperitoneally, and probiotics were gavaged for 35 days. After the treatment period, glucose and insulin levels were measured and changes in RAGE and TGFβ expression in kidney tissue were measured by real-time method . Results: Blood glucose, insulin levels, expression of RAGE and TGFβ genes in the tissues of all diabetic rats treated with probiotics showed significant changes compared with diabetic rats.Conclusion: According to the results of the present study, it is possible that the use of probiotics Lactobacillus casei and Bifidobacterium lactis in diabetic conditions is an effective method to reduce the expression of RAGE and TGFβ gene in the kidney tissue of type 1 diabetic rats.
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