Investigation the effect of carboplatin on oogenesis and uterus changes in adult female NMRI mice strain

Subject Areas : Developmental biology of plants and animals , development and differentiation in microorganisms

Sepideh Gholami Yarahmadi ¹ , nasim HAYATI ROODBARI ² , KAZEM PARIVAR ³ , Gholamhassan Vaezi ⁴

1 - islamic azad university science and research branch
2 - Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran
3 - Department of Biology, Science and Research Branch, Islamic Azad University,Tehran, Iran
4 - Department of Biology, Damghan Branch, Islamic Azad University, Damghan, Iran

Received: 2018-10-16 Accepted : 2019-05-05 Published : 2020-05-21

Keywords: ovarian follicles, Oogenesis, NMRI mouse strain, Carboplatin, uterine changes,

Abstract :

Cancer is one of the most common diseases around the world. The most important therapies for cancer are chemotherapy that is performed by various medications. One of the common drugs in chemotherapy is “Carboplatin”. In this study, the effects of Carboplatin on oogenesis and uterine changes in NMRI mouse strain have been investigated In vivo. In this study, 30 adult female mice were randomly divided into five groups: control, sham and three experimental groups. Experimental groups were injected Carboplatin with 0.36, 0.18, 0.09 mg/ml density for 28 days and all were injected subcutaneously. After describing the animals, the ovary and uterus were separated and subjected to histopathologic and stained by hematoxylin and eosin. Blood sampling was performed to study both estradiol and FSH. The results were analyzed using one-way ANOVA and SPSS software. By using Carboplatin,, the number of primordial, secondary, Graafian follicles, corpus lutea and blood vessels decreased and the number of atretic follicles increased. The diameter of ovary in experimental groups decreased, but there was no significant change in diameters of follicles. Uterine studies showed that, the uterine’ diameter and layers, and the diameter of lumen reduced. In addition, the number of uterine glands decreased. Also, the levels of FSH and estradiol decreased. Regarding to using Carboplatin, this drug by decrease in the number of primordial, secondary and Graafian follicles, increase in number of atretic follicles, and also disruption of the hormonal system, the probability of premature aging may increase and has a negative effect on fertility.