The effect of Dacarbazine on the development of ovarian follicles and sex hormones and Estradiol in NMRI mice

Subject Areas : Developmental biology of plants and animals , development and differentiation in microorganisms

nasim HAYATI ¹ , simin mohamadi gorji ² , kazem parivar ³ , zeinab ghaffari far ⁴

1 - Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran
2 - Department of Biology, , Islamic Azad University, Sari, Iran
3 - Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran
4 - Department of Biology, Science and Research Branch, Islamic Azad University,Tehran,Iran

Received: 2019-04-20 Accepted : 2019-07-28 Published : 2019-11-22

Keywords: Infertility, Oogenesis, NMRI, Gonadotropin, Dacarbazine,

Abstract :

After cancer treatment occurs fertility problems and many birth defects, such as abortion, early delivery, low birth weight. Therefore, preserving fertility should be an integral part of improving the quality of life in survivors of cancer. Dacarbazine is an anti-cancer chemotherapy drug and this study focuses on the effects of dacarbazine on oogenesis and sex hormones and estradiol in NMRI mice. 30 adult female mice NMRI were divided into 5 groups: control, sham, 1, 2 and 3 experimental groups received 10-day solvent and doses of 20, 30 and 40 mg / kg by intraperitoneal injection dacarbazine. For analysis sex hormones and estradiol was drawn blood from heart and ovarian tissue was removed from the body to investigate Oogenesis. The results of this research work show that Dacarbazine reduces the number of Graafian follicle and blood vessel in comparison with the control group and the number of atretic follicle in the experimental groups 1, 2 and 3 has a significant increase compared to the control group. Also, the levels of FSH, LH and estradiol hormones show a significant decrease with dose increase.